FastQCFastQC Report
Fri 10 Feb 2017
ERR1633018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268332
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5380.20049789067274867No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG4320.16099458879298778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.14795104571948184No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT3690.1375162112606771No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG3550.13229879403127467No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT3360.12521801350565717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC601.2605597E-930.83333236
TTAACGG706.544724E-926.4285735
AACGGCC751.3636964E-824.66666637
AGCCTTA751.3636964E-824.6666667
GAGGCAC751.3636964E-824.6666667
GCCTTAT551.8956205E-523.5454541
TTTAACG802.7061105E-823.12500234
GCACCGC802.7061105E-823.12500210
GGCCAAC400.001927351523.12500211
TATTAAG400.001927351523.1250022
ACTGTCC502.694628E-422.28
ACCGCCT855.1435563E-821.76470612
GTATTAG957.0831447E-921.4210531
GTATCAA4250.021.3294121
GTACATG705.077909E-621.1428571
TAGAAGT705.077909E-621.1428574
TAAAGCC1151.3278623E-1020.9130444
GCCTGCC1151.3278623E-1020.91304415
GTATTAA450.00381828320.5555551
CTCTAAT450.00381828320.5555551
Produced by FastQC (version 0.11.5)