##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633018.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268332
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67527167836859	33.0	33.0	33.0	27.0	33.0
2	31.22960362535963	33.0	33.0	33.0	27.0	33.0
3	31.661754841017842	33.0	33.0	33.0	27.0	33.0
4	31.79510084522159	33.0	33.0	33.0	27.0	33.0
5	31.91056601523486	33.0	33.0	33.0	33.0	33.0
6	34.80678785981545	37.0	37.0	37.0	27.0	37.0
7	34.99406705126485	37.0	37.0	37.0	33.0	37.0
8	35.16375981992457	37.0	37.0	37.0	33.0	37.0
9	35.25872799367947	37.0	37.0	37.0	33.0	37.0
10	35.29845489915478	37.0	37.0	37.0	33.0	37.0
11	35.32227613553359	37.0	37.0	37.0	33.0	37.0
12	35.28221755139156	37.0	37.0	37.0	33.0	37.0
13	35.30291206415932	37.0	37.0	37.0	33.0	37.0
14	35.267567789156715	37.0	37.0	37.0	33.0	37.0
15	35.28620887557205	37.0	37.0	37.0	33.0	37.0
16	35.29644619352146	37.0	37.0	37.0	33.0	37.0
17	35.249869564569266	37.0	37.0	37.0	33.0	37.0
18	35.25414039324419	37.0	37.0	37.0	33.0	37.0
19	35.262387639193236	37.0	37.0	37.0	33.0	37.0
20	35.288899572171786	37.0	37.0	37.0	33.0	37.0
21	35.273824963105405	37.0	37.0	37.0	33.0	37.0
22	35.28375296274764	37.0	37.0	37.0	33.0	37.0
23	35.27153675297765	37.0	37.0	37.0	33.0	37.0
24	35.26116154614433	37.0	37.0	37.0	33.0	37.0
25	35.26925599630309	37.0	37.0	37.0	33.0	37.0
26	35.237616087533354	37.0	37.0	37.0	33.0	37.0
27	35.17267787666026	37.0	37.0	37.0	33.0	37.0
28	35.142897604460146	37.0	37.0	37.0	33.0	37.0
29	35.18386550989073	37.0	37.0	37.0	33.0	37.0
30	35.15186410864153	37.0	37.0	37.0	33.0	37.0
31	35.13778080884874	37.0	37.0	37.0	33.0	37.0
32	35.17034867253999	37.0	37.0	37.0	33.0	37.0
33	35.1617287539317	37.0	37.0	37.0	33.0	37.0
34	35.07592832759418	37.0	37.0	37.0	33.0	37.0
35	35.08645633021779	37.0	37.0	37.0	33.0	37.0
36	35.064703427097776	37.0	37.0	37.0	33.0	37.0
37	35.09340294858608	37.0	37.0	37.0	33.0	37.0
38	34.96228552688461	37.0	37.0	37.0	27.0	37.0
39	35.020616251509324	37.0	37.0	37.0	27.0	37.0
40	35.06845251404976	37.0	37.0	37.0	27.0	37.0
41	35.09527376533548	37.0	37.0	37.0	33.0	37.0
42	35.01296900854166	37.0	37.0	37.0	27.0	37.0
43	34.80372076382988	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	8.0
17	11.0
18	8.0
19	8.0
20	15.0
21	46.0
22	141.0
23	333.0
24	704.0
25	1277.0
26	2034.0
27	3209.0
28	4671.0
29	6080.0
30	8108.0
31	10351.0
32	13272.0
33	17571.0
34	24701.0
35	45990.0
36	129782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82952461875587	19.755004993813635	12.943666800828824	25.47180358660167
2	16.352876287584035	22.37153973435893	37.11335211603536	24.162231862021674
3	18.65636599436519	25.200497890672747	29.67778721881848	26.46534889614358
4	13.255221143955994	17.034494581339533	38.103543371644086	31.606740903060388
5	14.68628415544922	36.27372061476082	34.75992427291564	14.280070956874319
6	31.484504270828673	38.621558367991895	16.431510218684316	13.462427142495118
7	28.157282769106928	30.976924108939674	22.443838230252076	18.421954891701326
8	25.39615103677534	35.2861380677668	20.317740709270605	18.99997018618726
9	25.77553180388474	15.1469075622736	19.842583068735745	39.234977565105915
10	14.870011776456032	27.92138097580609	33.52004233561409	23.688564912123784
11	34.847875020497	23.02669826930817	23.822354396792033	18.303072313402797
12	23.817509652221876	24.791303310823903	30.11605026608828	21.27513677086594
13	28.877659019423703	21.584082405378414	25.607829107225378	23.930429467972512
14	22.700609692470522	20.175379753439767	26.556653697658124	30.567356856431584
15	24.076889823055023	28.646974643352262	23.58570725817271	23.690428275420004
16	24.078380513691993	27.869579476171307	23.890553493433508	24.16148651670319
17	22.998002474546457	27.01392305054932	25.92199215896725	24.066082315936974
18	24.27030693320215	26.4057212706647	26.7210023403843	22.60296945574885
19	25.101366963314103	25.916774741737846	26.128452812187884	22.853405482760163
20	24.34521413771	26.679635675208324	26.074415276597644	22.900734910484026
21	23.71614268890777	26.726219757613702	26.799636271484577	22.75800128199395
22	23.394153511321793	26.154167225675657	26.676654293934384	23.77502496906817
23	23.47465080571829	26.063235096820357	26.726592430272945	23.735521667188408
24	23.32483639670259	26.598020362834102	25.691307782895816	24.38583545756749
25	24.146206937674226	27.00796028800143	25.937271737996216	22.90856103632813
26	24.260244771402593	26.11652728709211	26.25963358824143	23.363594353263867
27	23.764590134609364	26.482491838468764	26.036775338014102	23.71614268890777
28	23.460489244667055	25.85640177094048	26.815288523172786	23.867820461219683
29	24.075026459758806	26.052427589702308	25.99354530954191	23.879000640996974
30	23.68670154882757	26.38708763770255	26.45752277029948	23.468688043170403
31	23.760490735357692	26.364727278147964	26.610318560589118	23.26446342590522
32	23.624465214733988	26.316652505105615	26.366963314103426	23.691918966056974
33	23.868565806538168	25.97602969455749	26.92559963030872	23.22980486859562
34	24.184592221576256	25.664475351430315	26.24845340846414	23.902479018529284
35	23.52533428737534	26.760878314923303	26.595038981560158	23.118748416141198
36	24.26769822458745	25.575779258530478	26.81566119583203	23.340861321050042
37	24.148442973629685	25.824351922245576	26.06584380543506	23.96136129868968
38	24.11490243429781	26.15565791631263	26.503734180045612	23.225705469343946
39	23.83987001177646	26.001744108045255	26.385596947065576	23.77278893311271
40	23.36620306187857	26.81677921380976	26.581622765827404	23.235394958484264
41	24.107821653772195	25.559381661523783	26.761996332901035	23.57080035180299
42	23.82682646870295	26.156030588971866	26.39938583545757	23.61775710686761
43	23.907323763099445	25.39615103677534	26.84771104452693	23.848814155598287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	6.5
2	11.0
3	40.5
4	70.0
5	70.0
6	77.5
7	85.0
8	76.5
9	68.0
10	97.0
11	126.0
12	126.0
13	189.0
14	252.0
15	496.0
16	740.0
17	863.0
18	986.0
19	986.0
20	1001.5
21	1017.0
22	1278.0
23	1539.0
24	2103.0
25	2667.0
26	2667.0
27	3503.0
28	4339.0
29	5331.0
30	6323.0
31	7687.0
32	9051.0
33	9051.0
34	10790.5
35	12530.0
36	14131.5
37	15733.0
38	16552.0
39	17371.0
40	17371.0
41	18432.5
42	19494.0
43	20578.0
44	21662.0
45	21536.0
46	21410.0
47	21410.0
48	21022.0
49	20634.0
50	20706.0
51	20778.0
52	20265.5
53	19753.0
54	19753.0
55	18650.0
56	17547.0
57	15521.0
58	13495.0
59	12989.0
60	12483.0
61	12483.0
62	11644.0
63	10805.0
64	8738.0
65	6671.0
66	5480.0
67	4289.0
68	4289.0
69	3467.0
70	2645.0
71	2111.5
72	1578.0
73	1275.0
74	972.0
75	972.0
76	736.0
77	500.0
78	391.0
79	282.0
80	218.5
81	155.0
82	155.0
83	132.5
84	110.0
85	101.0
86	92.0
87	67.0
88	42.0
89	42.0
90	30.5
91	19.0
92	11.5
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	268332.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.96051796926692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9422871945288	58.00718814753149
2	6.300435783833912	8.31160015467578
3	1.9294402305753664	3.8180063099847894
4	0.9824107600784284	2.5920129037341737
5	0.5961814692151668	1.9662219256555484
6	0.39153355563886666	1.5495453679373061
7	0.2853225702676432	1.317401716622341
8	0.18720919562939334	0.9878732409859684
9	0.16435736174659282	0.9756987041580696
>10	1.1102601787929072	13.754742954923726
>50	0.08461354568336082	3.812671858008553
>100	0.02538406370500825	2.7068593507379943
>500	5.640903045557389E-4	0.20017736504426678
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	538	0.20049789067274867	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	432	0.16099458879298778	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	397	0.14795104571948184	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	369	0.1375162112606771	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	355	0.13229879403127467	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	336	0.12521801350565717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	3.726726592430273E-4	0.0	0.0
6	0.0	0.0	7.453453184860546E-4	0.0	0.0
7	0.0	0.0	0.001118017977729082	0.0	0.0
8	0.0	0.0	0.001118017977729082	0.0	0.0
9	0.0	0.0	0.0018633632962151364	0.001118017977729082	0.0
10	0.0	0.0	0.0018633632962151364	0.001118017977729082	0.0
11	0.0	0.0	0.002236035955458164	0.0014906906369721092	0.0
12	0.0	0.0	0.0029813812739442184	0.0014906906369721092	0.0
13	0.0	0.0	0.003354053933187246	0.0014906906369721092	0.0
14	0.0	0.0	0.003354053933187246	0.0014906906369721092	0.0
15	0.0	0.0	0.003354053933187246	0.0014906906369721092	0.0
16	0.0	0.0	0.003354053933187246	0.0014906906369721092	0.0
17	0.0	0.0	0.003726726592430273	0.0014906906369721092	0.0
18	0.0	0.0	0.0040993992516733	0.0018633632962151364	0.0
19	0.0	0.0	0.0040993992516733	0.0018633632962151364	0.0
20	0.0	0.0	0.0040993992516733	0.002236035955458164	0.0
21	0.0	0.0	0.0040993992516733	0.002236035955458164	0.0
22	0.0	0.0	0.004844744570159354	0.002236035955458164	0.0
23	0.0	0.0	0.004844744570159354	0.002608708614701191	0.0
24	0.0	0.0	0.004844744570159354	0.002608708614701191	0.0
25	0.0	0.0	0.004844744570159354	0.002608708614701191	0.0
26	0.0	0.0	0.004844744570159354	0.003354053933187246	0.0
27	0.0	0.0	0.004844744570159354	0.004844744570159354	0.0
28	0.0	0.0	0.004844744570159354	0.008944143821832656	0.0
29	0.0	0.0	0.004844744570159354	0.01788828764366531	0.0
30	0.0	0.0	0.004844744570159354	0.03130450337641429	0.0
31	0.0	0.0	0.004844744570159354	0.06521771536752978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	60	1.2605597E-9	30.833332	36
TTAACGG	70	6.544724E-9	26.42857	35
AACGGCC	75	1.3636964E-8	24.666666	37
AGCCTTA	75	1.3636964E-8	24.666666	7
GAGGCAC	75	1.3636964E-8	24.666666	7
GCCTTAT	55	1.8956205E-5	23.545454	1
TTTAACG	80	2.7061105E-8	23.125002	34
GCACCGC	80	2.7061105E-8	23.125002	10
GGCCAAC	40	0.0019273515	23.125002	11
TATTAAG	40	0.0019273515	23.125002	2
ACTGTCC	50	2.694628E-4	22.2	8
ACCGCCT	85	5.1435563E-8	21.764706	12
GTATTAG	95	7.0831447E-9	21.421053	1
GTATCAA	425	0.0	21.329412	1
GTACATG	70	5.077909E-6	21.142857	1
TAGAAGT	70	5.077909E-6	21.142857	4
TAAAGCC	115	1.3278623E-10	20.913044	4
GCCTGCC	115	1.3278623E-10	20.913044	15
GTATTAA	45	0.003818283	20.555555	1
CTCTAAT	45	0.003818283	20.555555	1
>>END_MODULE