FastQCFastQC Report
Fri 10 Feb 2017
ERR1633017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268880
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60602.253793513835168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48981.82163046712288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24940.927551324010711No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13580.5050580184468908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.461916096399881No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11670.4340226123177626No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11450.4258405236536745No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10400.3867896459387087No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10160.3778637310324308No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8170.3038530199345433No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7910.2941832787860756No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.2912079738173163No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7450.2770752752157096No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7150.2659178815828622No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6850.2547604879500149No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6520.24248735495388277No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6510.24211544183278785No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6220.2313299613210354No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6030.2242636120202321No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6030.2242636120202321No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5910.21980065456709313No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5850.21756917584052365No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5830.21682534959833383No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5770.21459387087176435No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5760.21422195775066943No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5730.21310621838738472No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5730.21310621838738472No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5720.21273430526628978No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5670.21087473966081524No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5660.2105028265397203No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5630.2093870871764356No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5620.20901517405534065No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5550.20641178220767628No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5550.20641178220767628No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5450.20269265099672718No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4990.18558464742636122No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4930.18335316869979174No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4910.1826093424576019No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4620.17182386194584945No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4560.16959238321927997No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4450.16550133888723592No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4430.1647575126450461No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.1643855995239512No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4140.15397203213329366No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3990.14839333531687No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3920.1457899434692056No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3880.14430229098482594No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3870.14393037786373103No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3860.14355846474263612No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3840.1428146385004463No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3780.1405831597738768No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3770.1402112466527819No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3750.13946742041059207No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3750.13946742041059207No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3710.13797976792621242No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3690.13723594168402262No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3690.13723594168402262No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3660.13612020232073788No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3560.13240107110978877No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC3540.13165724486759894No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3530.13128533174650403No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3490.12979767926212438No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3460.12868193989883964No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3450.12831002677774472No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3400.12645046117227016No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3390.12607854805117524No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3390.12607854805117524No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3330.12384706932460578No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3330.12384706932460578No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3320.12347515620351086No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3300.12273132996132104No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3250.12087176435584647No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3220.11975602499256174No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3220.11975602499256174No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3210.11938411187146683No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3210.11938411187146683No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3180.11826837250818209No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3130.11640880690270752No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3110.1156649806605177No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3100.11529306753942278No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3080.11454924129723296No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3080.11454924129723296No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3060.11380541505504314No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.11343350193394823No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3040.11306158881285332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.11157393632847366No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2970.11045819696518892No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC2920.10859863135971437No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2880.10711097887533472No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2840.10562332639095506No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2750.10227610830110087No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.10116036893781613No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2720.10116036893781613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT355.609927E-737.017
GGGTATT308.288302E-637.018
GATATCA200.001839429437.01
GTTATCG200.001839429437.011
GTATTGC352.3815577E-531.71428520
GGTATTG405.9234746E-527.7519
GGTATCA8800.025.2272721
GTATCAA25450.023.8428291
CGCCGTT652.6701728E-622.7692325
GCCGTTT652.6701728E-622.7692326
AGGACCG903.796231E-922.611115
ATAGATC502.694648E-422.26
CGGGGGG502.694648E-422.216
GAACGTA502.694648E-422.214
TGTCCGG2250.022.214
CCGGCAG1450.021.68965716
CCGCTCT1550.021.48387128
CGCTCTC1550.021.48387129
GCCGTCC1750.021.14285927
GACCGTT705.0779727E-621.1428577
Produced by FastQC (version 0.11.5)