FastQCFastQC Report
Fri 10 Feb 2017
ERR1633016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491397
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13930.28347751410773775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.20919948636235058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5330.10846627065285298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG405.9333157E-527.759
GTATCAA8900.025.5674171
GGTATCA2600.024.9038471
GCGCCTT601.3348035E-624.66666813
TTACACT802.717752E-823.1254
CGCGCGC502.6990517E-422.210
TATCGCG502.6990517E-422.27
TTAACGG909.4505594E-820.55555535
ATAGCAC555.1373354E-420.1818183
TAGCACT851.2423407E-619.5882344
CCGACGA500.0070292918.513
GACGATC500.0070292918.515
ATCGCGC609.226559E-418.58
ATCGAGC701.2173955E-418.536
TAGTACC500.0070292918.54
CGTATTA1258.543793E-917.762
TATACTG1054.783451E-717.619055
TCGTCTT752.0642331E-417.26666614
TCGAGCT752.0642331E-417.26666637
ATAGTAC752.0642331E-417.2666663
Produced by FastQC (version 0.11.5)