##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491397
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.725555915074775	33.0	33.0	33.0	27.0	33.0
2	31.250084961853656	33.0	33.0	33.0	27.0	33.0
3	31.688160489380277	33.0	33.0	33.0	27.0	33.0
4	31.8086638705568	33.0	33.0	33.0	27.0	33.0
5	31.925921403671573	33.0	33.0	33.0	33.0	33.0
6	34.83059115135013	37.0	37.0	37.0	27.0	37.0
7	34.997610893025396	37.0	37.0	37.0	33.0	37.0
8	35.182380030810116	37.0	37.0	37.0	33.0	37.0
9	35.28732369143483	37.0	37.0	37.0	33.0	37.0
10	35.30097863845322	37.0	37.0	37.0	33.0	37.0
11	35.34935093213837	37.0	37.0	37.0	33.0	37.0
12	35.30778169178892	37.0	37.0	37.0	33.0	37.0
13	35.33354497483705	37.0	37.0	37.0	33.0	37.0
14	35.31466207567405	37.0	37.0	37.0	33.0	37.0
15	35.33179079237358	37.0	37.0	37.0	33.0	37.0
16	35.30809711903003	37.0	37.0	37.0	33.0	37.0
17	35.28877669175839	37.0	37.0	37.0	33.0	37.0
18	35.29363223625704	37.0	37.0	37.0	33.0	37.0
19	35.30446258320666	37.0	37.0	37.0	33.0	37.0
20	35.29327814374121	37.0	37.0	37.0	33.0	37.0
21	35.307755236600954	37.0	37.0	37.0	33.0	37.0
22	35.30952162915118	37.0	37.0	37.0	33.0	37.0
23	35.304814640708024	37.0	37.0	37.0	33.0	37.0
24	35.30215894683932	37.0	37.0	37.0	33.0	37.0
25	35.289527612093686	37.0	37.0	37.0	33.0	37.0
26	35.26632844726362	37.0	37.0	37.0	33.0	37.0
27	35.20189988949871	37.0	37.0	37.0	33.0	37.0
28	35.175906649816746	37.0	37.0	37.0	33.0	37.0
29	35.197089115318164	37.0	37.0	37.0	33.0	37.0
30	35.18462668677261	37.0	37.0	37.0	33.0	37.0
31	35.17862135910476	37.0	37.0	37.0	33.0	37.0
32	35.19964102344947	37.0	37.0	37.0	33.0	37.0
33	35.1820849537136	37.0	37.0	37.0	33.0	37.0
34	35.132033773099955	37.0	37.0	37.0	33.0	37.0
35	35.13393447660446	37.0	37.0	37.0	33.0	37.0
36	35.11782530214877	37.0	37.0	37.0	33.0	37.0
37	35.15067654055682	37.0	37.0	37.0	33.0	37.0
38	34.99880951654161	37.0	37.0	37.0	27.0	37.0
39	35.06601383402829	37.0	37.0	37.0	27.0	37.0
40	35.11279678142113	37.0	37.0	37.0	33.0	37.0
41	35.13293121447628	37.0	37.0	37.0	33.0	37.0
42	35.05480497438934	37.0	37.0	37.0	33.0	37.0
43	34.8502900913111	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	26.0
17	27.0
18	23.0
19	10.0
20	40.0
21	84.0
22	235.0
23	579.0
24	1210.0
25	2159.0
26	3714.0
27	5609.0
28	8244.0
29	11121.0
30	14537.0
31	18554.0
32	23929.0
33	31879.0
34	45171.0
35	83130.0
36	241101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29186787872128	19.868456665384606	13.675297162986343	25.16437829290777
2	15.818370889525172	22.854229879303293	38.3984843212311	22.928914909940435
3	17.98952781559513	26.501993296662373	30.626560601713077	24.88191828602942
4	13.03589562003838	17.916470796525008	38.399501828460494	30.64813175497612
5	13.803910076781095	37.17116710114225	35.43611377358836	13.58880904848829
6	29.814589832660758	38.924942561716904	17.70055576244869	13.559911843173648
7	26.424459245782938	32.043744670805886	23.523546134795286	18.008249948615884
8	24.18024530064286	36.066154250025946	21.037165469060657	18.716434980270535
9	26.051441095488983	15.907911525711391	19.756937873043587	38.283709505756036
10	14.86700163004658	28.15727405743218	33.967443838688475	23.00828047383277
11	33.39784329167659	23.722774050309628	24.26714041803267	18.612242239981114
12	22.72561696550854	25.3591291766128	30.344100594834728	21.571153263043936
13	27.7075358620423	22.091913463045152	26.803582439453233	23.396968235459312
14	22.391264089931358	21.034519950264247	27.23337749314709	29.340838466657303
15	24.055702415765666	28.2669613367603	24.431162583410153	23.246173664063882
16	23.250447194427316	28.575062525819245	24.67088728665417	23.503602993099264
17	22.551419727837168	27.65096246008828	26.20182866399266	23.5957891480819
18	22.910803281257312	27.457432585058516	27.362600911279472	22.269163222404696
19	24.97471494534969	26.24130794449295	27.30541700498782	21.478560105169546
20	24.816797823348537	25.867882791307235	27.220556902056792	22.094762483287443
21	23.13567237895225	27.014613438828484	26.64566531745208	23.204048864767184
22	22.73497803201892	26.829020120187952	26.740089988339367	23.695911859453762
23	22.792772442648204	27.199596253131382	27.171513053600243	22.83611825062017
24	23.5243601405788	27.19267720397153	26.261861590526603	23.021101064923066
25	23.460664188019056	26.70671575121541	27.102119060555925	22.730501000209607
26	23.392694705095877	26.93972490674546	26.719536342305712	22.948044045852946
27	23.213002928385805	26.713838301821134	26.507894838592826	23.56526393120023
28	22.65805448547712	26.94644045445943	27.251896124721963	23.143608935341486
29	23.705272925964138	26.98286721327155	26.365850829370142	22.94600903139417
30	23.02293257793597	27.24233155676571	26.96902911495186	22.765706750346464
31	23.490985903454845	26.68127807048069	26.92731131854692	22.900424707517548
32	22.404491683913413	26.739072481109975	27.1224692051437	23.733966629832906
33	22.778730842882638	26.528041481734725	27.45783958795027	23.23538808743236
34	23.043893226861375	26.469229563876052	27.094182504166692	23.392694705095877
35	23.003599940577576	27.13935982515156	26.98449522483857	22.87254500943229
36	23.447029591145245	26.907775179742654	26.700000203501446	22.94519502561066
37	23.666200648355606	26.2710191555911	26.746195031715704	23.316585164337592
38	23.820658245776833	26.555514176928224	26.57871334175829	23.045114235536644
39	23.069737910487852	26.437076335427363	27.038423107996184	23.4547626460886
40	22.810273566993693	26.99853682460414	27.19674723288909	22.994442375513078
41	22.857689403883214	26.094990404906827	27.54860123281176	23.4987189583982
42	23.11939226328203	26.520918931129007	26.98368121905506	23.376007586533902
43	23.17250614065613	25.94399233206552	27.256576657977156	23.626924869301195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	12.5
2	18.0
3	82.0
4	146.0
5	146.0
6	186.0
7	226.0
8	211.5
9	197.0
10	276.5
11	356.0
12	356.0
13	513.5
14	671.0
15	1350.5
16	2030.0
17	2343.5
18	2657.0
19	2657.0
20	2608.5
21	2560.0
22	3075.0
23	3590.0
24	4748.0
25	5906.0
26	5906.0
27	7785.0
28	9664.0
29	12171.0
30	14678.0
31	17361.5
32	20045.0
33	20045.0
34	22339.5
35	24634.0
36	26972.0
37	29310.0
38	32173.5
39	35037.0
40	35037.0
41	36799.5
42	38562.0
43	37621.0
44	36680.0
45	37087.0
46	37494.0
47	37494.0
48	37028.0
49	36562.0
50	37473.0
51	38384.0
52	38672.0
53	38960.0
54	38960.0
55	34676.0
56	30392.0
57	27178.0
58	23964.0
59	20761.0
60	17558.0
61	17558.0
62	15801.5
63	14045.0
64	11577.0
65	9109.0
66	7791.5
67	6474.0
68	6474.0
69	5301.5
70	4129.0
71	3601.5
72	3074.0
73	2515.5
74	1957.0
75	1957.0
76	1466.0
77	975.0
78	799.0
79	623.0
80	466.0
81	309.0
82	309.0
83	239.5
84	170.0
85	154.0
86	138.0
87	109.0
88	80.0
89	80.0
90	51.0
91	22.0
92	12.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	491397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.571389911367845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2758754112511	53.46992469302221
2	6.551575183697257	7.936760299707359
3	1.7240847380809197	3.1329062673161365
4	0.832236693395192	2.016389330167507
5	0.47071057212288575	1.4255796799729175
6	0.3162557720571066	1.1493631008595007
7	0.2266872226779534	0.9611532106925968
8	0.17675649095555723	0.8565109066427379
9	0.14808197320953687	0.8072577844307627
>10	1.046288875611145	13.077901400806013
>50	0.14977980036960953	6.2821035563912995
>100	0.08065066159373746	8.277819838596361
>500	3.3886832602410694E-4	0.1094021169374857
>1k	6.777366520482139E-4	0.49692781445713474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1393	0.28347751410773775	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1028	0.20919948636235058	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.10846627065285298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.0350144587777296E-4	0.0	0.0
2	0.0	0.0	4.0700289175554593E-4	0.0	0.0
3	0.0	0.0	6.105043376333189E-4	0.0	0.0
4	0.0	0.0	6.105043376333189E-4	2.0350144587777296E-4	0.0
5	2.0350144587777296E-4	0.0	8.140057835110919E-4	2.0350144587777296E-4	0.0
6	2.0350144587777296E-4	0.0	0.0028490202422888217	2.0350144587777296E-4	0.0
7	2.0350144587777296E-4	0.0	0.005698040484577643	2.0350144587777296E-4	0.0
8	2.0350144587777296E-4	0.0	0.007122550605722053	2.0350144587777296E-4	0.0
9	2.0350144587777296E-4	0.0	0.00895406361862201	2.0350144587777296E-4	0.0
10	8.140057835110919E-4	0.0	0.00956456795625533	2.0350144587777296E-4	0.0
11	8.140057835110919E-4	0.0	0.013227593982055242	2.0350144587777296E-4	0.0
12	8.140057835110919E-4	0.0	0.015262608440832973	2.0350144587777296E-4	0.0
13	8.140057835110919E-4	0.0	0.01648361711609961	4.0700289175554593E-4	0.0
14	8.140057835110919E-4	0.0	0.01709412145373293	4.0700289175554593E-4	0.0
15	8.140057835110919E-4	0.0	0.01851863157487734	4.0700289175554593E-4	0.0
16	8.140057835110919E-4	0.0	0.019536138804266204	4.0700289175554593E-4	0.0
17	8.140057835110919E-4	0.0	0.020350144587777296	4.0700289175554593E-4	0.0
18	8.140057835110919E-4	0.0	0.020960648925410615	4.0700289175554593E-4	0.0
19	8.140057835110919E-4	0.0	0.02116415037128839	4.0700289175554593E-4	0.0
20	8.140057835110919E-4	0.0	0.021571153263043933	6.105043376333189E-4	0.0
21	8.140057835110919E-4	0.0	0.023199164830066118	8.140057835110919E-4	0.0
22	8.140057835110919E-4	0.0	0.023606167721821662	0.0016280115670221837	0.0
23	8.140057835110919E-4	0.0	0.023606167721821662	0.0020350144587777297	0.0
24	8.140057835110919E-4	0.0	0.023809669167699436	0.0026455187964110486	0.0
25	8.140057835110919E-4	0.0	0.023809669167699436	0.0032560231340443674	0.0
26	8.140057835110919E-4	0.0	0.023809669167699436	0.003663026025799913	0.0
27	8.140057835110919E-4	0.0	0.023809669167699436	0.004680533255188778	0.0
28	8.140057835110919E-4	0.0	0.023809669167699436	0.010175072293888648	0.0
29	8.140057835110919E-4	0.0	0.023809669167699436	0.02543768073472162	0.0
30	8.140057835110919E-4	0.0	0.023809669167699436	0.045787825322498914	0.0
31	8.140057835110919E-4	0.0	0.023809669167699436	0.1076522648693419	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCG	40	5.9333157E-5	27.75	9
GTATCAA	890	0.0	25.567417	1
GGTATCA	260	0.0	24.903847	1
GCGCCTT	60	1.3348035E-6	24.666668	13
TTACACT	80	2.717752E-8	23.125	4
CGCGCGC	50	2.6990517E-4	22.2	10
TATCGCG	50	2.6990517E-4	22.2	7
TTAACGG	90	9.4505594E-8	20.555555	35
ATAGCAC	55	5.1373354E-4	20.181818	3
TAGCACT	85	1.2423407E-6	19.588234	4
CCGACGA	50	0.00702929	18.5	13
GACGATC	50	0.00702929	18.5	15
ATCGCGC	60	9.226559E-4	18.5	8
ATCGAGC	70	1.2173955E-4	18.5	36
TAGTACC	50	0.00702929	18.5	4
CGTATTA	125	8.543793E-9	17.76	2
TATACTG	105	4.783451E-7	17.61905	5
TCGTCTT	75	2.0642331E-4	17.266666	14
TCGAGCT	75	2.0642331E-4	17.266666	37
ATAGTAC	75	2.0642331E-4	17.266666	3
>>END_MODULE