FastQCFastQC Report
Fri 10 Feb 2017
ERR1633015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505380
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52901.0467371087102775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.7665519015394356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19660.38901420713126755No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17050.33736989987732No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16520.32688274169931536No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16150.319561518065614No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13000.25723218172464285No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12460.2465471526376192No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11190.22141754719221182No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10790.21350271083145356No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10400.20578574537971428No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10240.20261981083541097No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9920.1962879417468044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.19549645811072855No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9680.19153903993034946No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9650.19094542720329258No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9350.1850092999327239No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9180.18164549447940165No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8980.17768807629902253No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8960.1772923344809846No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8750.17313704539158653No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8540.16898175630218845No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8480.16779453084807472No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8410.16640943448494203No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8080.15987969448731648No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7950.15730737267007003No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7850.15532866357988048No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7680.15196485812655822No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7640.1511733744904824No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7630.15097550358146344No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7550.1493925363093118No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7540.14919466540029286No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7330.14503937631089478No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7270.14385215085678105No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7260.1436542799477621No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7210.14266492540266731No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7150.14147769994855355No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7060.13969686176738297No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6950.13752028176817443No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6830.13514583085994697No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6810.13475008904190905No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6720.13296925086073844No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6710.1327713799517195No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6690.13237563813368158No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6680.13217776722466262No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6670.1319798963156437No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6580.13019905813447308No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6560.12980331631643516No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6540.12940757449839724No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6460.1278246072262456No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6460.1278246072262456No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6390.1264395108631129No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6320.12505441449998023No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6090.12050338359254423No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6030.1193161581384305No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6000.11872254541137363No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5980.11832680359333571No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5910.11694170723020302No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5900.11674383632118407No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5880.11634809450314615No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA5700.11278641814080492No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5670.11219280541374807No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5650.11179706359571016No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5640.1115991926866912No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5550.1098183545055206No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5540.10962048359650163No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.10487158178004671No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5270.10427796905298982No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5260.10408009814397087No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5230.103486485416914No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5200.10289287268985714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATATA200.001840960437.03
TCGTATG454.002415E-628.77777937
AACTCGA405.933645E-527.7535
CCGCTCT1300.027.03846228
GCTCTCT1300.027.03846230
GCCGCTC1400.026.42857227
GGTATCA8550.026.1812861
CGCTCTC1400.025.10714329
CTCGACT451.321779E-424.66666837
AGAGGCC451.321779E-424.66666837
GTATCAA25300.023.69171
CTCGTAT551.8999244E-523.54545436
ATACCGC551.8999244E-523.5454546
ATCCGAT400.001929698223.12526
TTCGCCG1600.023.12524
TTTATGT652.677909E-622.769234
TAATATA1400.022.4642874
GTTATCG502.6992E-422.211
AATATAG1250.022.1999995
CTCTCTT1600.021.9687531
Produced by FastQC (version 0.11.5)