FastQCFastQC Report
Fri 10 Feb 2017
ERR1633014.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633014.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439493
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11380.25893472706049925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.17679462471529694No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6000.13652094572609802No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5880.13379052681157605No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5160.11740801332444431No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5000.11376745477174835No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4710.10716894239498696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1150.025.7391335
GGTATCA2950.025.7118641
GTTTAAC1350.024.66666833
AACGGCC1150.024.13043637
GTATCAA8700.023.178161
ATTAGAC400.001929299423.1250023
GCACCGC1400.022.46428510
TTTAACG1350.021.92592634
CACCGCC1450.021.68965511
GAGGCAC1550.021.4838717
TAACGGC1301.8189894E-1221.34615336
AGCGAAC1400.021.14285727
ATAATAC450.00382210520.5555553
CTAAATT450.00382210520.55555514
TATTAGA1950.019.9230772
ACGGGCT759.24443E-619.73333416
ATTAGAG1700.019.5882343
GTATTAG2100.019.3809531
CGAACTA1457.2759576E-1219.1379329
GCGAACT1457.2759576E-1219.1379328
Produced by FastQC (version 0.11.5)