##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439493
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.659942251639958	33.0	33.0	33.0	27.0	33.0
2	31.175559110156478	33.0	33.0	33.0	27.0	33.0
3	31.633318391874273	33.0	33.0	33.0	27.0	33.0
4	31.760487652818135	33.0	33.0	33.0	27.0	33.0
5	31.86799107153015	33.0	33.0	33.0	33.0	33.0
6	34.7531746808254	37.0	37.0	37.0	27.0	37.0
7	34.9264334130464	37.0	37.0	37.0	27.0	37.0
8	35.10135087475796	37.0	37.0	37.0	33.0	37.0
9	35.16344515157238	37.0	37.0	37.0	33.0	37.0
10	35.23091152760112	37.0	37.0	37.0	33.0	37.0
11	35.26870962677449	37.0	37.0	37.0	33.0	37.0
12	35.203646019390526	37.0	37.0	37.0	33.0	37.0
13	35.24130077157088	37.0	37.0	37.0	33.0	37.0
14	35.21868152621316	37.0	37.0	37.0	33.0	37.0
15	35.23707772364975	37.0	37.0	37.0	33.0	37.0
16	35.226572436876125	37.0	37.0	37.0	33.0	37.0
17	35.18494037447695	37.0	37.0	37.0	33.0	37.0
18	35.189145219605315	37.0	37.0	37.0	33.0	37.0
19	35.201636886139255	37.0	37.0	37.0	33.0	37.0
20	35.214169508956914	37.0	37.0	37.0	33.0	37.0
21	35.198194282957864	37.0	37.0	37.0	33.0	37.0
22	35.19459240533979	37.0	37.0	37.0	33.0	37.0
23	35.205316125626574	37.0	37.0	37.0	33.0	37.0
24	35.19287451677273	37.0	37.0	37.0	33.0	37.0
25	35.191568466391956	37.0	37.0	37.0	33.0	37.0
26	35.129221625827945	37.0	37.0	37.0	33.0	37.0
27	35.06301579319807	37.0	37.0	37.0	33.0	37.0
28	35.060242142650736	37.0	37.0	37.0	27.0	37.0
29	35.09453392886804	37.0	37.0	37.0	33.0	37.0
30	35.0551772155643	37.0	37.0	37.0	27.0	37.0
31	35.055488938390376	37.0	37.0	37.0	27.0	37.0
32	35.1026774032806	37.0	37.0	37.0	33.0	37.0
33	35.06254934663351	37.0	37.0	37.0	27.0	37.0
34	35.01424823603561	37.0	37.0	37.0	27.0	37.0
35	35.01544734500891	37.0	37.0	37.0	27.0	37.0
36	34.98736498647305	37.0	37.0	37.0	27.0	37.0
37	35.00384989066948	37.0	37.0	37.0	27.0	37.0
38	34.86141531264434	37.0	37.0	37.0	27.0	37.0
39	34.95358060310403	37.0	37.0	37.0	27.0	37.0
40	34.99444814820714	37.0	37.0	37.0	27.0	37.0
41	34.99437306168699	37.0	37.0	37.0	27.0	37.0
42	34.92554147620098	37.0	37.0	37.0	27.0	37.0
43	34.680497755368116	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	27.0
17	18.0
18	19.0
19	14.0
20	35.0
21	90.0
22	266.0
23	579.0
24	1304.0
25	2308.0
26	3803.0
27	5638.0
28	7786.0
29	10706.0
30	13818.0
31	17587.0
32	22323.0
33	29342.0
34	41253.0
35	74693.0
36	207873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.606790096770595	20.552318239425883	13.873030969776536	24.96786069402698
2	15.552921206936174	23.32141808857024	38.56762223744178	22.55803846705181
3	17.54635454944675	27.083025213143326	30.858284432289025	24.512335805120898
4	12.916474210055679	18.183907365987626	38.66682745800274	30.232790965953953
5	13.60636005579156	37.62062194392175	35.45039397669588	13.322624023590821
6	29.207518663550957	39.7264575317468	17.724286848709763	13.341736955992472
7	25.984714204776864	32.531803691981445	23.8615859638265	17.62189613941519
8	23.6638581274332	36.780335522977616	20.94117539983572	18.614630949753465
9	25.75308366686159	16.36590343873509	20.115223678192827	37.7657892162105
10	14.75222586025261	28.611604735456535	34.07904107687722	22.557128327413633
11	33.20303167513476	24.04042840272769	24.471151986493528	18.285387935644025
12	22.79171681915298	25.979253366947823	29.974538843622085	21.254490970277114
13	27.243664859281036	22.579199213639352	26.926708730286947	23.250427196792668
14	22.019690871071894	21.42468708261565	27.521029914014555	29.0345921322979
15	23.835191914319452	28.871904671974296	24.679801498544915	22.613101915161334
16	23.026987915620953	28.99613873258505	24.90528859390252	23.07158475789148
17	22.436762360265124	27.96426791780529	26.199507159385927	23.399462562543658
18	22.378740958331534	28.05073118343182	27.547423963521606	22.023103894715046
19	24.65295237921878	26.648888605734335	27.58564982832491	21.112509186721972
20	24.27934005774836	26.288928378836523	27.781102315622775	21.65062924779234
21	22.709121646988688	27.703740446377985	26.70986796149199	22.877269945141332
22	22.38056123760788	27.58223680468176	26.825228160630544	23.211973797079814
23	22.314576113840264	27.62296555349004	27.502599586341535	22.559858746328153
24	23.31254422709804	27.88895386274639	26.162874038949425	22.63562787120614
25	23.29616171361091	27.08257014332424	27.325577426716695	22.295690716348155
26	23.183987003205967	27.333541148550715	26.980179434029665	22.502292414213652
27	22.961912931491515	27.189056480990597	26.641835023538484	23.207195563979404
28	22.222879545294237	27.382461154102565	27.67302323358961	22.72163606701358
29	23.501398202019143	27.409765343247788	26.69029995927125	22.398536495461816
30	22.792171888972064	27.58747010760126	27.102593215364067	22.517764788062607
31	23.118912019076525	27.09053386515826	27.213857786130834	22.576696329634373
32	22.08340064574407	27.1801826195184	27.40066394686605	23.33575278787148
33	22.34210783789503	26.891895889126786	27.731272170432746	23.034724102545432
34	22.547571861212806	27.084617957510133	27.414088506529115	22.95372167474795
35	22.88682641134216	27.728769286427767	26.93285217284462	22.45155212938545
36	23.31572971583165	27.490312701226188	26.53807910478665	22.65587847815551
37	23.515050296591756	26.537168965148478	26.738537360094472	23.209243378165294
38	23.441328985899663	27.025913950847908	26.87551337563966	22.65724368761277
39	22.76919086310817	27.00270539007447	27.35083380167602	22.877269945141332
40	22.663387130170445	27.406352319604636	27.31420068123952	22.6160598689854
41	22.52823139390161	26.762200990686996	27.678256536509117	23.03131107890228
42	22.877497480050877	27.031602323586494	27.108054053193108	22.982846143169517
43	22.976930235521383	26.245469211113715	27.389287201388875	23.388313351976027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.0
2	18.0
3	63.5
4	109.0
5	109.0
6	139.5
7	170.0
8	159.0
9	148.0
10	210.0
11	272.0
12	272.0
13	426.5
14	581.0
15	1130.0
16	1679.0
17	1975.5
18	2272.0
19	2272.0
20	2275.0
21	2278.0
22	2884.0
23	3490.0
24	4574.5
25	5659.0
26	5659.0
27	7452.0
28	9245.0
29	12249.5
30	15254.0
31	17065.0
32	18876.0
33	18876.0
34	21060.5
35	23245.0
36	25644.0
37	28043.0
38	30347.0
39	32651.0
40	32651.0
41	34865.0
42	37079.0
43	35546.5
44	34014.0
45	34066.0
46	34118.0
47	34118.0
48	33239.0
49	32360.0
50	33138.0
51	33916.0
52	33495.0
53	33074.0
54	33074.0
55	29322.5
56	25571.0
57	22326.5
58	19082.0
59	16650.0
60	14218.0
61	14218.0
62	12556.0
63	10894.0
64	9044.5
65	7195.0
66	6133.0
67	5071.0
68	5071.0
69	4193.0
70	3315.0
71	2823.0
72	2331.0
73	1910.0
74	1489.0
75	1489.0
76	1138.5
77	788.0
78	592.0
79	396.0
80	314.0
81	232.0
82	232.0
83	188.0
84	144.0
85	126.0
86	108.0
87	84.0
88	60.0
89	60.0
90	46.5
91	33.0
92	19.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	439493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.72208708493265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34649981000419	50.995443556820476
2	6.115174783386629	7.059613027724543
3	1.7445756934080812	3.021016503034741
4	0.834024509592326	1.9256654149462598
5	0.48771511357448866	1.4075967129192226
6	0.34951465240785834	1.2104829122247818
7	0.2567718388615692	1.0374984510608052
8	0.2159367822689046	0.9971457400772682
9	0.15806700334885596	0.8211561599301304
>10	1.2202551686048946	14.23378154922299
>50	0.1832286985867119	7.521362809327467
>100	0.0862486498754247	8.93895983210565
>500	0.0015898368640631282	0.5691953736481821
>1k	3.9745921601578205E-4	0.26108195695752967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.25893472706049925	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	777	0.17679462471529694	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	600	0.13652094572609802	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	588	0.13379052681157605	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	516	0.11740801332444431	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	500	0.11376745477174835	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	471	0.10716894239498696	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	4.5506981908699344E-4	0.0	0.0
4	0.0	0.0	4.5506981908699344E-4	2.2753490954349672E-4	0.0
5	0.0	0.0	4.5506981908699344E-4	2.2753490954349672E-4	0.0
6	0.0	0.0	0.0013652094572609802	2.2753490954349672E-4	0.0
7	0.0	0.0	0.002957953824065457	2.2753490954349672E-4	0.0
8	0.0	0.0	0.0031854887336089537	2.2753490954349672E-4	0.0
9	0.0	0.0	0.003413023643152451	2.2753490954349672E-4	0.0
10	2.2753490954349672E-4	0.0	0.004095628371782941	2.2753490954349672E-4	0.0
11	2.2753490954349672E-4	0.0	0.0063709774672179075	2.2753490954349672E-4	0.0
12	2.2753490954349672E-4	0.0	0.007508652014935392	2.2753490954349672E-4	0.0
13	2.2753490954349672E-4	0.0	0.008191256743565882	2.2753490954349672E-4	0.0
14	2.2753490954349672E-4	0.0	0.008418791653109377	2.2753490954349672E-4	0.0
15	2.2753490954349672E-4	0.0	0.009101396381739868	2.2753490954349672E-4	0.0
16	2.2753490954349672E-4	0.0	0.009328931291283366	2.2753490954349672E-4	0.0
17	2.2753490954349672E-4	0.0	0.009328931291283366	2.2753490954349672E-4	0.0
18	2.2753490954349672E-4	0.0	0.009556466200826862	2.2753490954349672E-4	0.0
19	2.2753490954349672E-4	0.0	0.009556466200826862	2.2753490954349672E-4	0.0
20	2.2753490954349672E-4	0.0	0.009556466200826862	2.2753490954349672E-4	0.0
21	2.2753490954349672E-4	0.0	0.010011536019913855	2.2753490954349672E-4	0.0
22	2.2753490954349672E-4	0.0	0.010011536019913855	2.2753490954349672E-4	0.0
23	2.2753490954349672E-4	0.0	0.010011536019913855	2.2753490954349672E-4	0.0
24	2.2753490954349672E-4	0.0	0.010011536019913855	2.2753490954349672E-4	0.0
25	2.2753490954349672E-4	0.0	0.010011536019913855	4.5506981908699344E-4	0.0
26	2.2753490954349672E-4	0.0	0.010011536019913855	4.5506981908699344E-4	0.0
27	2.2753490954349672E-4	0.0	0.010011536019913855	6.826047286304901E-4	0.0
28	2.2753490954349672E-4	0.0	0.010011536019913855	0.005233302919500424	0.0
29	2.2753490954349672E-4	0.0	0.010011536019913855	0.018885397492110226	0.0
30	2.2753490954349672E-4	0.0	0.010011536019913855	0.03526791097924199	0.0
31	2.2753490954349672E-4	0.0	0.010011536019913855	0.08851107981242022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	115	0.0	25.73913	35
GGTATCA	295	0.0	25.711864	1
GTTTAAC	135	0.0	24.666668	33
AACGGCC	115	0.0	24.130436	37
GTATCAA	870	0.0	23.17816	1
ATTAGAC	40	0.0019292994	23.125002	3
GCACCGC	140	0.0	22.464285	10
TTTAACG	135	0.0	21.925926	34
CACCGCC	145	0.0	21.689655	11
GAGGCAC	155	0.0	21.483871	7
TAACGGC	130	1.8189894E-12	21.346153	36
AGCGAAC	140	0.0	21.142857	27
ATAATAC	45	0.003822105	20.555555	3
CTAAATT	45	0.003822105	20.555555	14
TATTAGA	195	0.0	19.923077	2
ACGGGCT	75	9.24443E-6	19.733334	16
ATTAGAG	170	0.0	19.588234	3
GTATTAG	210	0.0	19.380953	1
CGAACTA	145	7.2759576E-12	19.13793	29
GCGAACT	145	7.2759576E-12	19.13793	28
>>END_MODULE