FastQCFastQC Report
Fri 10 Feb 2017
ERR1633013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510242
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15050.2949580787155898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.20049309935285609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5460.10700804716193492No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5210.10210841130287196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2850.029.2105261
GTATCAA9500.025.5105271
CCGTCTA551.8999712E-523.54545426
GACCGTG400.001929723223.1250027
GCCGCTC652.6779926E-622.7692327
TTCGCCG652.6779926E-622.7692324
GGACCGT603.7210604E-521.5833326
GTCTAAA450.003822936220.5555551
AATATAC656.8925205E-519.9230773
ACCCGTC656.8925205E-519.92307724
TGGGGCG759.2492155E-619.73333437
AGCTTCG851.2425317E-619.58823621
GTACCCT1002.8671457E-718.51
CGGTTGG801.6138003E-518.533
ACTTATA500.007029650718.531
CCGCTCT801.6138003E-518.528
TATACAC1205.153197E-918.53
CTCTAGC701.21751116E-418.52
TAACCGT953.5984413E-617.5263167
GTACATG1703.6379788E-1217.4117641
Produced by FastQC (version 0.11.5)