##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633013.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510242
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717933843156775	33.0	33.0	33.0	27.0	33.0
2	31.227252950560715	33.0	33.0	33.0	27.0	33.0
3	31.675077708224723	33.0	33.0	33.0	27.0	33.0
4	31.800567181847047	33.0	33.0	33.0	27.0	33.0
5	31.920373077872853	33.0	33.0	33.0	33.0	33.0
6	34.783549766581345	37.0	37.0	37.0	27.0	37.0
7	34.958059117046425	37.0	37.0	37.0	27.0	37.0
8	35.14338882334265	37.0	37.0	37.0	33.0	37.0
9	35.23897483938994	37.0	37.0	37.0	33.0	37.0
10	35.26226770826392	37.0	37.0	37.0	33.0	37.0
11	35.29553623574696	37.0	37.0	37.0	33.0	37.0
12	35.24801760733142	37.0	37.0	37.0	33.0	37.0
13	35.2896351143183	37.0	37.0	37.0	33.0	37.0
14	35.24988926822959	37.0	37.0	37.0	33.0	37.0
15	35.287187256243115	37.0	37.0	37.0	33.0	37.0
16	35.28072561647218	37.0	37.0	37.0	33.0	37.0
17	35.2293774326692	37.0	37.0	37.0	33.0	37.0
18	35.24008411694843	37.0	37.0	37.0	33.0	37.0
19	35.25624115615727	37.0	37.0	37.0	33.0	37.0
20	35.26021966047483	37.0	37.0	37.0	33.0	37.0
21	35.26418640566633	37.0	37.0	37.0	33.0	37.0
22	35.26850396478534	37.0	37.0	37.0	33.0	37.0
23	35.25350323963923	37.0	37.0	37.0	33.0	37.0
24	35.257630692886906	37.0	37.0	37.0	33.0	37.0
25	35.2633495478616	37.0	37.0	37.0	33.0	37.0
26	35.204063953966944	37.0	37.0	37.0	33.0	37.0
27	35.15518322678258	37.0	37.0	37.0	33.0	37.0
28	35.13463415398967	37.0	37.0	37.0	33.0	37.0
29	35.17741581445667	37.0	37.0	37.0	33.0	37.0
30	35.138563661948645	37.0	37.0	37.0	33.0	37.0
31	35.12388827262358	37.0	37.0	37.0	33.0	37.0
32	35.16371839244907	37.0	37.0	37.0	33.0	37.0
33	35.14113695070182	37.0	37.0	37.0	33.0	37.0
34	35.07945641479925	37.0	37.0	37.0	33.0	37.0
35	35.06752874126395	37.0	37.0	37.0	33.0	37.0
36	35.04701886555791	37.0	37.0	37.0	27.0	37.0
37	35.093475252919205	37.0	37.0	37.0	33.0	37.0
38	34.93801764652851	37.0	37.0	37.0	27.0	37.0
39	35.01141027198859	37.0	37.0	37.0	27.0	37.0
40	35.03751161213699	37.0	37.0	37.0	27.0	37.0
41	35.05914644423626	37.0	37.0	37.0	27.0	37.0
42	34.988642644078695	37.0	37.0	37.0	27.0	37.0
43	34.764750843717295	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	27.0
17	25.0
18	22.0
19	15.0
20	36.0
21	117.0
22	274.0
23	656.0
24	1335.0
25	2476.0
26	4136.0
27	6110.0
28	8686.0
29	11976.0
30	15593.0
31	19967.0
32	25267.0
33	33282.0
34	47314.0
35	86093.0
36	246823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19809815734495	20.10614571125074	13.786595380231342	24.909160751172973
2	15.728811034763895	23.253279816244056	38.522113036559126	22.495796112432924
3	17.940310676110553	26.834129687481628	30.665840914703217	24.559718721704606
4	12.982271157607567	17.99401068512588	38.70026379639465	30.3234543608719
5	13.86126583072346	37.12748068563545	35.30383621889221	13.707417264748884
6	29.235735200159922	39.264113891055615	17.996558495772593	13.503592413011864
7	26.433927430513364	32.305455058580044	23.448481308869123	17.812136202037465
8	24.14305368824989	36.18263490657374	20.94515935575668	18.729152049419685
9	26.04607225590994	15.903434056780899	20.017560294918884	38.03293339239028
10	14.956432437941212	28.260903649640756	33.9141035038276	22.868560408590433
11	33.318111797931174	24.162260260817416	24.157556610392717	18.36207133085869
12	22.818192151959266	25.702901760341174	30.09081965028359	21.38808643741597
13	27.798965980848305	22.25199023208595	26.629128923138435	23.319914863927313
14	22.3976074098173	21.261871817686508	27.428945480771873	28.91157529172432
15	24.191266105103068	28.498046025219402	24.46760556755422	22.843082302123303
16	23.081400590308128	28.591531077410327	24.83880198023683	23.488266352044715
17	22.569878606621955	27.711556477122617	26.042936488960137	23.675628427295283
18	23.010845833937623	27.33820422466202	27.47931373740304	22.17163620399732
19	25.046546540661097	26.32437157270472	27.27901662348454	21.35006526314964
20	24.61733843940718	25.901435005350404	27.513415202982117	21.9678113522603
21	22.963613344256256	27.19219507606195	26.763966901979845	23.080224677701953
22	22.688057823542554	27.102433747123918	26.705563242539814	23.503945186793718
23	22.61534722739406	27.278232681747095	27.312922103629255	22.79349798722959
24	23.48709043943854	27.274508958494202	26.43314348877591	22.80525711329134
25	23.519624021542718	26.745152300281045	27.21042172145766	22.524801956718576
26	23.37439881468009	26.9707315352323	26.945841385068263	22.70902826501934
27	23.398701008541046	26.68282893215376	26.70517127167109	23.213298787634105
28	22.63161401844615	27.041286291602805	27.307826482335834	23.01927320761521
29	23.555097385162334	26.974063287616463	26.6842008301943	22.7866384970269
30	23.100411177441295	27.130459664237755	27.009928622104805	22.75920053621615
31	23.383022173792043	26.811983333398665	26.95054503549296	22.85444945731633
32	22.186139126140144	26.932122404662884	27.29136370584938	23.590374763347587
33	22.644353071679713	26.562297890020815	27.660012307885278	23.133336730414193
34	22.936567354314228	26.41570078511765	27.316645826882148	23.331086033685978
35	23.032796202586223	27.186511498465432	26.912719846661	22.867972452287347
36	23.22309805935223	26.938589923996847	27.04050234986536	22.797809666785565
37	23.652306160606145	26.268907694780125	26.744172373109233	23.3346137715045
38	23.64172294715057	26.63206870465387	26.886065827587696	22.840142520607866
39	22.98556371290486	26.487431454094327	27.192979017799395	23.334025815201414
40	22.940487063001477	26.905860356458305	27.265493628513525	22.888158952026686
41	22.774879370965152	26.332406975513578	27.550064479207904	23.342649174313365
42	22.966553125771693	26.691060320396986	27.01424030166078	23.32814625217054
43	23.02868050846461	26.049208022859744	27.33879218096511	23.583319287710538
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	20.0
2	26.0
3	79.0
4	132.0
5	132.0
6	183.0
7	234.0
8	220.5
9	207.0
10	304.5
11	402.0
12	402.0
13	602.5
14	803.0
15	1477.0
16	2151.0
17	2510.0
18	2869.0
19	2869.0
20	2751.0
21	2633.0
22	3191.5
23	3750.0
24	4984.5
25	6219.0
26	6219.0
27	8085.5
28	9952.0
29	13262.5
30	16573.0
31	18925.0
32	21277.0
33	21277.0
34	23887.5
35	26498.0
36	28774.5
37	31051.0
38	33496.5
39	35942.0
40	35942.0
41	38145.5
42	40349.0
43	39318.5
44	38288.0
45	38634.0
46	38980.0
47	38980.0
48	38729.0
49	38478.0
50	39045.5
51	39613.0
52	39478.5
53	39344.0
54	39344.0
55	35141.0
56	30938.0
57	27439.0
58	23940.0
59	21206.5
60	18473.0
61	18473.0
62	16160.0
63	13847.0
64	11481.5
65	9116.0
66	7732.0
67	6348.0
68	6348.0
69	5333.0
70	4318.0
71	3652.0
72	2986.0
73	2501.5
74	2017.0
75	2017.0
76	1492.5
77	968.0
78	805.5
79	643.0
80	506.5
81	370.0
82	370.0
83	293.5
84	217.0
85	181.5
86	146.0
87	114.0
88	82.0
89	82.0
90	61.0
91	40.0
92	23.0
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	510242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.660064858661066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4952586280533	53.681281280616986
2	6.337825816867082	7.689058502281074
3	1.7542518927044974	3.192391007696513
4	0.7992899981355858	1.9393993251113482
5	0.4825021014431519	1.4634304383990928
6	0.3171296588142642	1.1542263403366992
7	0.22834424278702783	0.969596360429407
8	0.17563039854759244	0.8523001093639561
9	0.13683235781148212	0.7470233729647221
>10	1.0420912400930864	13.021961906387533
>50	0.14977163036354318	6.332354292052857
>100	0.0794440818817853	8.147608097639186
>500	9.767714985465404E-4	0.310080350842206
>1k	6.51180999031027E-4	0.49928861587840556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1505	0.2949580787155898	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1023	0.20049309935285609	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.10700804716193492	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	521	0.10210841130287196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.879563030875546E-4	0.0	0.0
2	0.0	0.0	5.879563030875546E-4	0.0	0.0
3	0.0	0.0	5.879563030875546E-4	0.0	0.0
4	0.0	0.0	7.839417374500727E-4	1.9598543436251818E-4	0.0
5	0.0	0.0	9.799271718125909E-4	1.9598543436251818E-4	0.0
6	1.9598543436251818E-4	0.0	0.0017638689092626637	1.9598543436251818E-4	0.0
7	1.9598543436251818E-4	0.0	0.004507664990337918	1.9598543436251818E-4	0.0
8	1.9598543436251818E-4	0.0	0.005095621293425473	1.9598543436251818E-4	0.0
9	1.9598543436251818E-4	0.0	0.006271533899600582	1.9598543436251818E-4	0.0
10	5.879563030875546E-4	0.0	0.0068594902026881364	1.9598543436251818E-4	0.0
11	7.839417374500727E-4	0.0	0.01058321345557598	1.9598543436251818E-4	0.0
12	7.839417374500727E-4	0.0	0.01195511149611361	1.9598543436251818E-4	0.0
13	7.839417374500727E-4	0.0	0.012543067799201163	1.9598543436251818E-4	0.0
14	7.839417374500727E-4	0.0	0.013327009536651236	1.9598543436251818E-4	0.0
15	7.839417374500727E-4	0.0	0.015678834749001454	1.9598543436251818E-4	0.0
16	7.839417374500727E-4	0.0	0.017442703658264117	3.9197086872503635E-4	0.0
17	7.839417374500727E-4	0.0	0.017442703658264117	5.879563030875546E-4	0.0
18	9.799271718125909E-4	0.0	0.017638689092626635	5.879563030875546E-4	0.0
19	9.799271718125909E-4	0.0	0.01822664539571419	5.879563030875546E-4	0.0
20	9.799271718125909E-4	0.0	0.01861861626443923	5.879563030875546E-4	0.0
21	9.799271718125909E-4	0.0	0.019794528870614336	9.799271718125909E-4	0.0
22	9.799271718125909E-4	0.0	0.019990514304976854	0.0011759126061751091	0.0
23	9.799271718125909E-4	0.0	0.02018649973933937	0.0015678834749001454	0.0
24	9.799271718125909E-4	0.0	0.02057847060806441	0.0017638689092626637	0.0
25	9.799271718125909E-4	0.0	0.02057847060806441	0.0019598543436251817	0.0
26	9.799271718125909E-4	0.0	0.020774456042426927	0.0023518252123502182	0.0
27	9.799271718125909E-4	0.0	0.020774456042426927	0.0029397815154377726	0.0
28	9.799271718125909E-4	0.0	0.020774456042426927	0.010191242586850946	0.0
29	9.799271718125909E-4	0.0	0.020774456042426927	0.02449817929531477	0.0
30	9.799271718125909E-4	0.0	0.020774456042426927	0.03998102860995371	0.0
31	9.799271718125909E-4	0.0	0.020774456042426927	0.11543542083952321	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	285	0.0	29.210526	1
GTATCAA	950	0.0	25.510527	1
CCGTCTA	55	1.8999712E-5	23.545454	26
GACCGTG	40	0.0019297232	23.125002	7
GCCGCTC	65	2.6779926E-6	22.76923	27
TTCGCCG	65	2.6779926E-6	22.76923	24
GGACCGT	60	3.7210604E-5	21.583332	6
GTCTAAA	45	0.0038229362	20.555555	1
AATATAC	65	6.8925205E-5	19.923077	3
ACCCGTC	65	6.8925205E-5	19.923077	24
TGGGGCG	75	9.2492155E-6	19.733334	37
AGCTTCG	85	1.2425317E-6	19.588236	21
GTACCCT	100	2.8671457E-7	18.5	1
CGGTTGG	80	1.6138003E-5	18.5	33
ACTTATA	50	0.0070296507	18.5	31
CCGCTCT	80	1.6138003E-5	18.5	28
TATACAC	120	5.153197E-9	18.5	3
CTCTAGC	70	1.21751116E-4	18.5	2
TAACCGT	95	3.5984413E-6	17.526316	7
GTACATG	170	3.6379788E-12	17.411764	1
>>END_MODULE