FastQCFastQC Report
Fri 10 Feb 2017
ERR1633012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395394
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9690.24507200412752847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.185637617161616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG706.5647328E-926.42857235
GGTATCA2150.024.0930231
GACCGTT400.001928958923.1257
TAACGGC855.1590177E-821.76470636
GTATCAA5400.021.5833341
GTTTCGG450.003821435920.55555530
TTTCGGG450.003821435920.55555531
CTACACT450.003821435920.5555554
GGACCGT450.003821435920.5555556
CCGCCGT450.003821435920.55555524
AACGGCC951.6691774E-719.47368437
ATTAGAG1153.0322553E-919.3043483
GTTTATA500.0070269218.51
TTTAACG1002.8630893E-718.534
AGGACCG701.21663164E-418.55
GGTTTCG500.0070269218.529
GGATAGT609.222211E-418.56
AGACGTA500.0070269218.524
CCCGTTC500.0070269218.526
CCAACGG609.222211E-418.526
Produced by FastQC (version 0.11.5)