##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633012.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395394
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49469895850721	33.0	33.0	33.0	27.0	33.0
2	31.03629797113765	33.0	33.0	33.0	27.0	33.0
3	31.504661173411836	33.0	33.0	33.0	27.0	33.0
4	31.6491170832132	33.0	33.0	33.0	27.0	33.0
5	31.757730263989842	33.0	33.0	33.0	27.0	33.0
6	34.59070193275568	37.0	37.0	37.0	27.0	37.0
7	34.790487463138035	37.0	37.0	37.0	27.0	37.0
8	34.95465029818359	37.0	37.0	37.0	27.0	37.0
9	35.04951263802688	37.0	37.0	37.0	27.0	37.0
10	35.10228531540691	37.0	37.0	37.0	33.0	37.0
11	35.1281860625098	37.0	37.0	37.0	33.0	37.0
12	35.064778423547146	37.0	37.0	37.0	33.0	37.0
13	35.10222967470422	37.0	37.0	37.0	33.0	37.0
14	35.075145298107714	37.0	37.0	37.0	33.0	37.0
15	35.105598466339906	37.0	37.0	37.0	33.0	37.0
16	35.09335498262492	37.0	37.0	37.0	33.0	37.0
17	35.04702651026571	37.0	37.0	37.0	27.0	37.0
18	35.05043070962129	37.0	37.0	37.0	27.0	37.0
19	35.06703187200615	37.0	37.0	37.0	33.0	37.0
20	35.0676717400871	37.0	37.0	37.0	33.0	37.0
21	35.0644344628396	37.0	37.0	37.0	33.0	37.0
22	35.04655103516998	37.0	37.0	37.0	27.0	37.0
23	35.06284111544434	37.0	37.0	37.0	33.0	37.0
24	35.04982877838308	37.0	37.0	37.0	27.0	37.0
25	35.03289883002777	37.0	37.0	37.0	27.0	37.0
26	34.9862567464352	37.0	37.0	37.0	27.0	37.0
27	34.92302614607202	37.0	37.0	37.0	27.0	37.0
28	34.90081538920671	37.0	37.0	37.0	27.0	37.0
29	34.95187837954041	37.0	37.0	37.0	27.0	37.0
30	34.91676403789638	37.0	37.0	37.0	27.0	37.0
31	34.895734381401844	37.0	37.0	37.0	27.0	37.0
32	34.94192122288148	37.0	37.0	37.0	27.0	37.0
33	34.900033890246185	37.0	37.0	37.0	27.0	37.0
34	34.84314885911268	37.0	37.0	37.0	27.0	37.0
35	34.83560954389799	37.0	37.0	37.0	27.0	37.0
36	34.81919553660399	37.0	37.0	37.0	27.0	37.0
37	34.84822480867186	37.0	37.0	37.0	27.0	37.0
38	34.69397360607394	37.0	37.0	37.0	27.0	37.0
39	34.781471140179164	37.0	37.0	37.0	27.0	37.0
40	34.817339160432375	37.0	37.0	37.0	27.0	37.0
41	34.82019959837529	37.0	37.0	37.0	27.0	37.0
42	34.7305421933565	37.0	37.0	37.0	27.0	37.0
43	34.50446390182957	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	15.0
16	20.0
17	13.0
18	10.0
19	11.0
20	38.0
21	98.0
22	294.0
23	649.0
24	1308.0
25	2446.0
26	3885.0
27	5690.0
28	8009.0
29	10595.0
30	13636.0
31	16928.0
32	21278.0
33	27661.0
34	38661.0
35	68238.0
36	175910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91918440846346	20.132323707491768	13.394993348406906	25.55349853563787
2	16.124422727709575	22.95558354451509	37.31139066349009	23.608603064285244
3	17.980798899325737	26.36155328608932	30.096561910398233	25.561085904186708
4	13.174959660490549	17.996985285563262	38.066586746384615	30.761468307561575
5	14.425105085054401	36.68947935477018	34.983838904990975	13.90157665518445
6	30.381341143264695	38.91181960272539	17.459546680020434	13.247292573989489
7	26.939457857225957	31.749596604905488	23.320788883999253	17.990156653869306
8	24.6690642751281	35.96741478120558	20.5382479248547	18.825273018811618
9	26.116987106531713	15.429925593205764	19.759783911743728	38.6933033885188
10	14.836593372686485	28.13522714052312	33.960555800037426	23.06762368675296
11	34.81236437578719	23.151843477645084	23.776030996929645	18.25976114963808
12	23.124528950869262	25.078023439910567	30.572037006125534	21.225410603094634
13	28.45035584758494	21.850862683804003	25.674390607849386	24.02439086076167
14	22.125272512987046	20.618674031472406	27.26596761711103	29.990085838429515
15	24.050693738397648	28.582123148049792	23.750233943863588	23.61694916968897
16	23.44243969306565	28.035832612533323	24.812212628416212	23.709515065984814
17	22.715063961516865	27.195658001891783	26.254571389550673	23.834706647040672
18	23.427770780537895	26.769500801731944	27.314273863538645	22.488454554191513
19	24.835733470917617	26.480927884591065	26.6134539219108	22.069884722580515
20	24.616458519856145	26.231809283904155	26.602072869087547	22.549659327152156
21	23.701674785151013	26.666565501752682	26.505966200802238	23.125793512294067
22	23.430299903387507	26.446531813836327	26.714113011325413	23.409055271450754
23	23.489228465783498	26.441726480422062	27.020642700698545	23.0484023530959
24	23.51629008027436	26.86054922431802	25.953352858161733	23.669807837245887
25	23.483158570944425	26.4526017086754	27.151651264308512	22.912588456071664
26	23.541581308770493	26.722712029014094	26.67415287030152	23.061553791913887
27	23.454832395028756	26.585127745995134	26.623823325594216	23.336216533381894
28	23.301820462627152	26.491297288274477	26.83272887297228	23.374153376126092
29	23.989488965437005	26.581839886290638	26.417952725635697	23.01071842263666
30	23.65513892471813	26.391649847999716	26.825900241278323	23.127310986003835
31	23.987718579442276	26.201206897423834	26.563883114058385	23.247191409075505
32	22.978851474731535	26.449819673540826	26.897221505637415	23.674107346090228
33	23.23656909310713	26.53454528900287	27.05175091174879	23.177134706141217
34	23.67081948638573	25.99811833259989	26.83551090810685	23.49555127290753
35	23.37744123583059	26.674405782586486	26.852456031199264	23.09569695038367
36	23.93890650844474	26.24748984557176	26.530751604728447	23.282852041255055
37	23.509714360865363	26.27632184605735	26.654425712074538	23.559538081002746
38	23.66677288982635	26.378245496896767	26.4761225511768	23.47885906210008
39	23.365807270722367	26.266711179228817	26.90784382160579	23.459637728443024
40	23.398432955482377	26.55376662265993	27.063131964572047	22.984668457285647
41	23.17966382899083	26.14101377360304	26.879011821120198	23.80031057628593
42	23.078751827291256	26.513047744781154	27.026965507822574	23.38123492010501
43	23.50844979944056	25.80995159258866	27.05377421002848	23.627824397942305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	7.5
2	13.0
3	56.0
4	99.0
5	99.0
6	128.5
7	158.0
8	153.0
9	148.0
10	186.5
11	225.0
12	225.0
13	376.0
14	527.0
15	926.0
16	1325.0
17	1547.0
18	1769.0
19	1769.0
20	1723.0
21	1677.0
22	1978.0
23	2279.0
24	3239.5
25	4200.0
26	4200.0
27	5662.0
28	7124.0
29	8952.0
30	10780.0
31	12929.0
32	15078.0
33	15078.0
34	17253.5
35	19429.0
36	21472.0
37	23515.0
38	25367.0
39	27219.0
40	27219.0
41	28889.0
42	30559.0
43	31137.0
44	31715.0
45	31631.5
46	31548.0
47	31548.0
48	31117.5
49	30687.0
50	30457.0
51	30227.0
52	29221.0
53	28215.0
54	28215.0
55	26427.0
56	24639.0
57	21956.0
58	19273.0
59	17814.5
60	16356.0
61	16356.0
62	14235.5
63	12115.0
64	10262.0
65	8409.0
66	7132.0
67	5855.0
68	5855.0
69	4916.5
70	3978.0
71	3246.5
72	2515.0
73	2049.0
74	1583.0
75	1583.0
76	1228.0
77	873.0
78	676.5
79	480.0
80	404.0
81	328.0
82	328.0
83	278.0
84	228.0
85	181.5
86	135.0
87	103.5
88	72.0
89	72.0
90	51.0
91	30.0
92	17.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	395394.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.47525519202848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.5692819799956	64.01987286886944
2	5.837007223619397	8.344031617318201
3	1.6409741533437654	3.518671391213055
4	0.7256634210410156	2.07467912809708
5	0.4655884920710242	1.6639028142624097
6	0.33017486377369815	1.415959958773099
7	0.23198245969312278	1.1606703854648288
8	0.15626531689375078	0.893528272211523
9	0.13811126634279558	0.8884384206070977
>10	0.8341270321354051	11.01151625987949
>50	0.04982578268185369	2.4321207140644727
>100	0.020286211513382606	2.143400181065274
>500	7.117968952064073E-4	0.43320798817400447
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	969	0.24507200412752847	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	734	0.185637617161616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.058245699226594E-4	0.0	0.0
2	0.0	0.0	5.058245699226594E-4	0.0	0.0
3	0.0	0.0	5.058245699226594E-4	0.0	0.0
4	0.0	0.0	5.058245699226594E-4	0.0	0.0
5	0.0	0.0	0.0012645614248066486	0.0	0.0
6	0.0	0.0	0.0015174737097679782	0.0	0.0
7	0.0	0.0	0.003540771989458616	0.0	0.0
8	0.0	0.0	0.004046596559381275	2.529122849613297E-4	0.0
9	0.0	0.0	0.004552421129303935	2.529122849613297E-4	0.0
10	2.529122849613297E-4	0.0	0.005311157984187924	2.529122849613297E-4	0.0
11	2.529122849613297E-4	0.0	0.00809319311876255	2.529122849613297E-4	0.0
12	2.529122849613297E-4	0.0	0.00885192997364654	2.529122849613297E-4	0.0
13	2.529122849613297E-4	0.0	0.00910484225860787	5.058245699226594E-4	0.0
14	2.529122849613297E-4	0.0	0.010116491398453189	5.058245699226594E-4	0.0
15	2.529122849613297E-4	0.0	0.011381052823259836	5.058245699226594E-4	0.0
16	2.529122849613297E-4	0.0	0.012392701963105155	5.058245699226594E-4	0.0
17	2.529122849613297E-4	0.0	0.012392701963105155	7.587368548839891E-4	0.0
18	2.529122849613297E-4	0.0	0.012645614248066485	7.587368548839891E-4	0.0
19	2.529122849613297E-4	0.0	0.013404351102950475	7.587368548839891E-4	0.0
20	2.529122849613297E-4	0.0	0.013404351102950475	7.587368548839891E-4	0.0
21	2.529122849613297E-4	0.0	0.013657263387911804	0.0010116491398453188	0.0
22	2.529122849613297E-4	0.0	0.013910175672873134	0.0010116491398453188	0.0
23	2.529122849613297E-4	0.0	0.014163087957834464	0.0012645614248066486	0.0
24	2.529122849613297E-4	0.0	0.014163087957834464	0.001770385994729308	0.0
25	2.529122849613297E-4	0.0	0.014163087957834464	0.0020232982796906376	0.0
26	2.529122849613297E-4	0.0	0.014163087957834464	0.0020232982796906376	0.0
27	2.529122849613297E-4	0.0	0.014163087957834464	0.003287859704497286	0.0
28	2.529122849613297E-4	0.0	0.014163087957834464	0.00859901768868521	0.0
29	2.529122849613297E-4	0.0	0.014163087957834464	0.01922133365706106	0.0
30	2.529122849613297E-4	0.0	0.014163087957834464	0.0374310181742768	0.0
31	2.529122849613297E-4	0.0	0.014163087957834464	0.09332463315073067	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	70	6.5647328E-9	26.428572	35
GGTATCA	215	0.0	24.093023	1
GACCGTT	40	0.0019289589	23.125	7
TAACGGC	85	5.1590177E-8	21.764706	36
GTATCAA	540	0.0	21.583334	1
GTTTCGG	45	0.0038214359	20.555555	30
TTTCGGG	45	0.0038214359	20.555555	31
CTACACT	45	0.0038214359	20.555555	4
GGACCGT	45	0.0038214359	20.555555	6
CCGCCGT	45	0.0038214359	20.555555	24
AACGGCC	95	1.6691774E-7	19.473684	37
ATTAGAG	115	3.0322553E-9	19.304348	3
GTTTATA	50	0.00702692	18.5	1
TTTAACG	100	2.8630893E-7	18.5	34
AGGACCG	70	1.21663164E-4	18.5	5
GGTTTCG	50	0.00702692	18.5	29
GGATAGT	60	9.222211E-4	18.5	6
AGACGTA	50	0.00702692	18.5	24
CCCGTTC	50	0.00702692	18.5	26
CCAACGG	60	9.222211E-4	18.5	26
>>END_MODULE