FastQCFastQC Report
Fri 10 Feb 2017
ERR1633011.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633011.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346107
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57711.6674034330423828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45341.3099995088224161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22820.6593336742683621No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11810.3412239567532584No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11000.3178207895246268No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10680.3085750938293649No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.3033743900007801No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9740.2814158627245332No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8080.2334538163053622No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7790.22507490458153115No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6830.19733781749574553No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6030.17422357825759086No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.173645722276637No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5640.16295538662899045No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5390.1557321868670671No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5290.1528429069622978No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5190.14995362705752846No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5180.14966469906705152No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5160.14908684308609765No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5010.14475292322894365No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5000.14446399523846673No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4950.14301935528608203No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4910.1418636433241743No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4610.13319580360986633No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4540.13117330767652777No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4500.13001759571462004No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4480.12943973973366618No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4390.1268393878193738No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4360.12597260384794298No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4360.12597260384794298No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4320.12481689188603524No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4290.12395010791460444No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4280.1236611799241275No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4270.12337225193365056No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4160.12019404403840431No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4140.11961618805745045No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4110.11874940408601964No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4060.11730476413363497No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4010.11586012418125032No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3980.11499334020981951No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3920.11325977226695791No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.11297084427648098No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3880.11210406030505017No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3860.1115262043240963No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3850.11123727633361936No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3820.11037049236218856No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3820.11037049236218856No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3810.11008156437171163No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3790.10950370839075778No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3760.10863692441932697No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3720.10748121245741923No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3720.10748121245741923No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3720.10748121245741923No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3650.1054587165240807No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3640.10516978853360376No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3570.10314729260026524No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3480.10054694068597284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATAG200.001840159337.05
GCTACCA200.001840159337.036
GAATCAA554.984031E-1033.6363641
CCCCTGC250.005491103529.59999810
GAGATGT250.005491103529.5999984
ACGGTGA250.005491103529.59999829
GCATATT250.005491103529.59999821
TCTAAGG453.997624E-628.77777722
GGTATCA8050.028.2670821
CTAAGGA405.9283226E-527.75000223
TAAGGAT405.9283226E-527.75000224
ATAAAAC405.9283226E-527.7500023
GTGACAT405.9283226E-527.75000216
AAGGATG405.9283226E-527.75000225
AGATATA659.352152E-825.6153851
GTATCAA26850.025.4245821
GGCAGCT2950.025.08474518
GACATCT451.3206033E-424.66666618
GCAGCTT3050.024.26229519
CGCTCTC3000.024.05000129
Produced by FastQC (version 0.11.5)