##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633011.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346107
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307104450357837	33.0	33.0	33.0	27.0	33.0
2	30.8958616843924	33.0	33.0	33.0	27.0	33.0
3	31.322339623295687	33.0	33.0	33.0	27.0	33.0
4	31.480877300950283	33.0	33.0	33.0	27.0	33.0
5	31.610392161961474	33.0	33.0	33.0	27.0	33.0
6	34.35634355849491	37.0	33.0	37.0	27.0	37.0
7	34.593952159303335	37.0	37.0	37.0	27.0	37.0
8	34.76913786776922	37.0	37.0	37.0	27.0	37.0
9	34.872293828209195	37.0	37.0	37.0	27.0	37.0
10	34.84531951101827	37.0	37.0	37.0	27.0	37.0
11	34.856850627118206	37.0	37.0	37.0	27.0	37.0
12	34.80251482922911	37.0	37.0	37.0	27.0	37.0
13	34.857093326630206	37.0	37.0	37.0	27.0	37.0
14	34.82239596425383	37.0	37.0	37.0	27.0	37.0
15	34.86217268070279	37.0	37.0	37.0	27.0	37.0
16	34.86610787993308	37.0	37.0	37.0	27.0	37.0
17	34.83654187866758	37.0	37.0	37.0	27.0	37.0
18	34.83488632128215	37.0	37.0	37.0	27.0	37.0
19	34.843614835874455	37.0	37.0	37.0	27.0	37.0
20	34.822046361385354	37.0	37.0	37.0	27.0	37.0
21	34.82303160583288	37.0	37.0	37.0	27.0	37.0
22	34.80848408151236	37.0	37.0	37.0	27.0	37.0
23	34.82538925823517	37.0	37.0	37.0	27.0	37.0
24	34.80675340284941	37.0	37.0	37.0	27.0	37.0
25	34.77950749334743	37.0	37.0	37.0	27.0	37.0
26	34.72819388223873	37.0	37.0	37.0	27.0	37.0
27	34.64589563343128	37.0	37.0	37.0	27.0	37.0
28	34.60773691372899	37.0	37.0	37.0	27.0	37.0
29	34.6235528319277	37.0	37.0	37.0	27.0	37.0
30	34.56609372246155	37.0	37.0	37.0	27.0	37.0
31	34.518264582918	37.0	37.0	37.0	27.0	37.0
32	34.53842019953367	37.0	37.0	37.0	27.0	37.0
33	34.50172345546319	37.0	37.0	37.0	27.0	37.0
34	34.393022966885965	37.0	37.0	37.0	27.0	37.0
35	34.36647915240085	37.0	37.0	37.0	27.0	37.0
36	34.322423412412924	37.0	37.0	37.0	27.0	37.0
37	34.29869086727515	37.0	37.0	37.0	27.0	37.0
38	34.11271658764486	37.0	37.0	37.0	27.0	37.0
39	34.14510830465723	37.0	37.0	37.0	27.0	37.0
40	34.1066808819238	37.0	37.0	37.0	27.0	37.0
41	34.07109939989656	37.0	37.0	37.0	27.0	37.0
42	33.91055945126796	37.0	37.0	37.0	27.0	37.0
43	33.616618560156255	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	5.0
16	22.0
17	28.0
18	18.0
19	30.0
20	53.0
21	156.0
22	370.0
23	782.0
24	1608.0
25	2801.0
26	4357.0
27	6208.0
28	8715.0
29	11044.0
30	14066.0
31	17529.0
32	21393.0
33	26769.0
34	35973.0
35	60768.0
36	133411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.395519304723685	18.745359094152963	14.444088099922855	22.415033501200497
2	16.7933615904908	24.142245028271603	37.80016006610673	21.26423331513087
3	18.58471513144779	29.003169540055534	30.28023125796358	22.1318840705331
4	11.861938649030503	18.747092662095827	40.09453723848405	29.29643145038962
5	14.66222873273294	36.2974455876362	32.870470692589286	16.169854987041578
6	25.969136712057256	42.38111335511851	16.354191044965862	15.295558887858379
7	25.720369712256613	33.49426622402898	21.446546877121815	19.338817186592586
8	25.288711294484074	32.869026052636904	19.50697327705016	22.33528937582886
9	28.3371905220062	13.196497037043459	19.033420300658467	39.43289214029188
10	17.34665869225413	26.355722363315394	30.81590375230781	25.481715192122667
11	35.14780111352847	24.57997093384416	21.370269887635903	18.90195806499146
12	24.71461137740641	26.99916499810752	27.269601597193933	21.016622027292136
13	30.645436237926422	22.78775060891574	24.707099249654007	21.85971390350383
14	23.90965799593767	21.37171452758829	29.088114369255752	25.630513107218288
15	26.604200435125552	26.20808016018168	26.635404658097062	20.552314746595705
16	21.112835048120957	27.92344563964323	25.877835467066546	25.08588384516927
17	22.54245074500082	25.182096865998087	25.14944800307419	27.126004385926894
18	25.138179811445593	22.91314535678273	28.54550760314007	23.40316722863161
19	27.97169661405288	23.338447358764775	28.63103028832124	20.058825738861103
20	26.735373742802082	22.428324188762435	28.999124548188853	21.837177520246627
21	23.312443839621853	25.648426642627857	29.033506979055613	22.00562253869468
22	23.356071966183865	25.09224026095976	28.939605382150607	22.612082390705766
23	22.721297171106045	25.339562620808014	30.29409980150647	21.64504040657947
24	23.087080007049842	25.47044700049407	28.61427246487358	22.82820052758251
25	23.09777034269749	25.53025509452279	30.66219406137408	20.709780501405632
26	23.09777034269749	25.643225938799276	29.268116507322876	21.990887211180357
27	23.495046329603273	25.14511408321704	29.531040978656893	21.8287986085228
28	22.011112170513744	25.430863865798724	29.70642026887639	22.851603694811143
29	23.841759918175594	25.32424943731274	28.00203405305296	22.83195659145871
30	23.959353610299704	24.73917025659695	29.723178092324048	21.5782980407793
31	23.251191105640743	24.917149898730738	29.223332668798957	22.608326326829562
32	20.986284588292058	24.91050455494977	30.0970509120012	24.00615994475697
33	21.24834227565464	25.1280673317789	30.576093520211955	23.047496872354504
34	21.987420075294633	25.010184711664312	30.05111136151537	22.951283851525684
35	22.09143415186633	26.333763836039143	29.13318713577015	22.441614876324373
36	22.88858647759219	25.685409425408906	29.145033183379702	22.2809709136192
37	22.800463440496724	25.68396478545652	28.9647421173221	22.550829656724655
38	22.3072633607526	25.800980621599678	28.29182882750132	23.5999271901464
39	21.917210573608738	25.237282112179184	29.136654271655875	23.708853042556203
40	21.961994412132665	26.82898641171661	29.60934046407614	21.599678712074592
41	22.889164333573145	25.01394077554051	28.683037326607092	23.413857564279255
42	21.485552155836203	26.78015758132601	28.899155463483833	22.835134799353956
43	22.64068626176298	25.73221575986617	28.58682430577827	23.04027367259258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	37.0
2	55.0
3	255.5
4	456.0
5	456.0
6	618.5
7	781.0
8	835.5
9	890.0
10	1166.5
11	1443.0
12	1443.0
13	2292.0
14	3141.0
15	5463.5
16	7786.0
17	8140.5
18	8495.0
19	8495.0
20	6444.5
21	4394.0
22	3117.0
23	1840.0
24	1793.0
25	1746.0
26	1746.0
27	1920.5
28	2095.0
29	2686.0
30	3277.0
31	4677.5
32	6078.0
33	6078.0
34	8508.5
35	10939.0
36	12133.5
37	13328.0
38	15151.5
39	16975.0
40	16975.0
41	19688.0
42	22401.0
43	24516.5
44	26632.0
45	28773.0
46	30914.0
47	30914.0
48	32428.0
49	33942.0
50	34572.0
51	35202.0
52	34006.0
53	32810.0
54	32810.0
55	29535.0
56	26260.0
57	22379.0
58	18498.0
59	16479.0
60	14460.0
61	14460.0
62	11635.5
63	8811.0
64	6426.5
65	4042.0
66	3846.5
67	3651.0
68	3651.0
69	2955.5
70	2260.0
71	1718.5
72	1177.0
73	1123.5
74	1070.0
75	1070.0
76	621.5
77	173.0
78	104.0
79	35.0
80	26.5
81	18.0
82	18.0
83	12.0
84	6.0
85	5.5
86	5.0
87	3.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	346107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.121092610088787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1374270251843	20.378091168338113
2	9.139429837644352	4.957426460603224
3	3.9417053735032175	3.2071006942939615
4	2.199897728725444	2.38654520133947
5	1.5404610729961223	2.088949371148229
6	1.1068734819107684	1.8011770926332031
7	0.8128435675629607	1.5431643971373015
8	0.6455874206332296	1.4007228978321733
9	0.5134870243320407	1.2533696226889373
>10	3.9875143818979844	22.08941165593299
>50	0.5518387522904504	10.556273060065239
>100	0.40162781778667916	21.079897257206586
>500	0.013849235096092385	2.3501402745393767
>1k	0.006391954659734946	3.2403274131988082
>5k	0.0010653257766224911	1.6674034330423828
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5771	1.6674034330423828	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4534	1.3099995088224161	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2282	0.6593336742683621	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1181	0.3412239567532584	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1100	0.3178207895246268	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1068	0.3085750938293649	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1050	0.3033743900007801	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	974	0.2814158627245332	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	808	0.2334538163053622	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	779	0.22507490458153115	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	683	0.19733781749574553	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	603	0.17422357825759086	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.173645722276637	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	564	0.16295538662899045	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	539	0.1557321868670671	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	529	0.1528429069622978	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	519	0.14995362705752846	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	518	0.14966469906705152	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	516	0.14908684308609765	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	501	0.14475292322894365	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	500	0.14446399523846673	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	495	0.14301935528608203	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	491	0.1418636433241743	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	461	0.13319580360986633	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	454	0.13117330767652777	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	450	0.13001759571462004	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	448	0.12943973973366618	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	439	0.1268393878193738	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	436	0.12597260384794298	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	436	0.12597260384794298	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	432	0.12481689188603524	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	429	0.12395010791460444	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	428	0.1236611799241275	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	427	0.12337225193365056	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	416	0.12019404403840431	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	414	0.11961618805745045	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	411	0.11874940408601964	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	406	0.11730476413363497	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	401	0.11586012418125032	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	398	0.11499334020981951	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	392	0.11325977226695791	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	391	0.11297084427648098	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	388	0.11210406030505017	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	386	0.1115262043240963	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	385	0.11123727633361936	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	382	0.11037049236218856	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	382	0.11037049236218856	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	381	0.11008156437171163	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	379	0.10950370839075778	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	376	0.10863692441932697	No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG	372	0.10748121245741923	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	372	0.10748121245741923	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	372	0.10748121245741923	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	365	0.1054587165240807	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	364	0.10516978853360376	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	357	0.10314729260026524	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	348	0.10054694068597284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0026003519142924008	0.0	0.0
2	5.778559809538669E-4	0.0	0.0026003519142924008	0.0	0.0
3	5.778559809538669E-4	0.0	0.003178207895246268	0.0	0.0
4	5.778559809538669E-4	0.0	0.004333919857154002	2.8892799047693346E-4	0.0
5	5.778559809538669E-4	0.0	0.008378911723831069	2.8892799047693346E-4	0.0
6	5.778559809538669E-4	0.0	0.015602111485754406	2.8892799047693346E-4	0.0
7	5.778559809538669E-4	0.0	0.036404926800093614	5.778559809538669E-4	0.0
8	8.667839714308003E-4	0.0	0.04391705455249388	5.778559809538669E-4	0.0
9	0.0011557119619077338	0.0	0.05229596627632495	0.002022495933338534	0.0
10	0.004911775838107868	0.0	0.059519166038248285	0.0023114239238154677	0.0
11	0.005489631819061735	0.0	0.09563516484786497	0.0023114239238154677	0.0
12	0.005778559809538669	0.0	0.10488086054312684	0.0023114239238154677	0.0
13	0.005778559809538669	0.0	0.1123929882955271	0.0026003519142924008	0.0
14	0.005778559809538669	0.0	0.12250546796221978	0.0026003519142924008	0.0
15	0.005778559809538669	0.0	0.13261794762891244	0.0026003519142924008	0.0
16	0.005778559809538669	0.0	0.14590863519085137	0.0026003519142924008	0.0
17	0.005778559809538669	0.0	0.15053148303848232	0.0026003519142924008	0.0
18	0.005778559809538669	0.0	0.15428754691468247	0.0026003519142924008	0.0
19	0.005778559809538669	0.0	0.15688789882897486	0.0026003519142924008	0.0
20	0.005778559809538669	0.0	0.16179967466708273	0.0028892799047693343	0.0
21	0.005778559809538669	0.0	0.16844501844805218	0.004044991866677068	0.0
22	0.005778559809538669	0.0	0.17220108232425232	0.004911775838107868	0.0
23	0.005778559809538669	0.0	0.173645722276637	0.006934271771446402	0.0
24	0.005778559809538669	0.0	0.17566821820997552	0.00924569569526187	0.0
25	0.005778559809538669	0.0	0.17595714620045247	0.01011247966669267	0.0
26	0.005778559809538669	0.0	0.17624607419092939	0.011268191628600405	0.0
27	0.005778559809538669	0.0	0.17624607419092939	0.014157471533369739	0.0
28	0.005778559809538669	0.0	0.17624607419092939	0.03900527871438601	0.0
29	0.005778559809538669	0.0	0.17624607419092939	0.09823551676215737	0.0
30	0.005778559809538669	0.0	0.17624607419092939	0.18029106605760647	0.0
31	0.005778559809538669	0.0	0.17653500218140633	0.33660110890562744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATATAG	20	0.0018401593	37.0	5
GCTACCA	20	0.0018401593	37.0	36
GAATCAA	55	4.984031E-10	33.636364	1
CCCCTGC	25	0.0054911035	29.599998	10
GAGATGT	25	0.0054911035	29.599998	4
ACGGTGA	25	0.0054911035	29.599998	29
GCATATT	25	0.0054911035	29.599998	21
TCTAAGG	45	3.997624E-6	28.777777	22
GGTATCA	805	0.0	28.267082	1
CTAAGGA	40	5.9283226E-5	27.750002	23
TAAGGAT	40	5.9283226E-5	27.750002	24
ATAAAAC	40	5.9283226E-5	27.750002	3
GTGACAT	40	5.9283226E-5	27.750002	16
AAGGATG	40	5.9283226E-5	27.750002	25
AGATATA	65	9.352152E-8	25.615385	1
GTATCAA	2685	0.0	25.424582	1
GGCAGCT	295	0.0	25.084745	18
GACATCT	45	1.3206033E-4	24.666666	18
GCAGCTT	305	0.0	24.262295	19
CGCTCTC	300	0.0	24.050001	29
>>END_MODULE