FastQCFastQC Report
Fri 10 Feb 2017
ERR1633010.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633010.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437482
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20150.4605903785755757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.35315738704678135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7160.16366387645663136No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4490.1026327940349546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2900.029.9827581
GTGACGC250.005492669529.5999983
GTATCAA10050.025.7711431
TCGTATG451.3213826E-424.66666637
CCGGCAG802.716115E-823.12500216
GGACCGT400.001929285523.1250026
GTATAGA957.1140676E-921.4210531
ACGAGAC957.1140676E-921.42105322
ACCGTAC806.939845E-720.812527
CGAACTA450.003822077320.55555529
CCTAGAG450.003822077320.5555552
GCCGGCA909.444557E-820.55555515
CATCGAC450.003822077320.55555515
TCCTAGA656.8896465E-519.9230771
GAGACCG851.241704E-619.58823624
TTCGCCG951.670196E-719.47368424
AGCTTCG1002.8648174E-718.49999821
CGGCAGC1054.780486E-717.61904717
CCGCTCT1054.780486E-717.61904728
GCTTCGC1054.780486E-717.61904722
Produced by FastQC (version 0.11.5)