##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633010.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	437482
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23344503316708	33.0	33.0	33.0	27.0	33.0
2	30.876657782491623	33.0	33.0	33.0	27.0	33.0
3	31.32530709834919	33.0	33.0	33.0	27.0	33.0
4	31.475205837040154	33.0	33.0	33.0	27.0	33.0
5	31.592673527139404	33.0	33.0	33.0	27.0	33.0
6	34.344334166891436	37.0	33.0	37.0	27.0	37.0
7	34.55021006578556	37.0	37.0	37.0	27.0	37.0
8	34.71714264815467	37.0	37.0	37.0	27.0	37.0
9	34.811827686624824	37.0	37.0	37.0	27.0	37.0
10	34.859795374438264	37.0	37.0	37.0	27.0	37.0
11	34.85972451438002	37.0	37.0	37.0	27.0	37.0
12	34.817725072117256	37.0	37.0	37.0	27.0	37.0
13	34.860606836395554	37.0	37.0	37.0	27.0	37.0
14	34.82855066037003	37.0	37.0	37.0	27.0	37.0
15	34.85682382360874	37.0	37.0	37.0	27.0	37.0
16	34.84195921203615	37.0	37.0	37.0	27.0	37.0
17	34.79478698552169	37.0	37.0	37.0	27.0	37.0
18	34.82120178658779	37.0	37.0	37.0	27.0	37.0
19	34.80370849543524	37.0	37.0	37.0	27.0	37.0
20	34.821908101361885	37.0	37.0	37.0	27.0	37.0
21	34.812572860140534	37.0	37.0	37.0	27.0	37.0
22	34.799955198156724	37.0	37.0	37.0	27.0	37.0
23	34.79076624866852	37.0	37.0	37.0	27.0	37.0
24	34.79241660228306	37.0	37.0	37.0	27.0	37.0
25	34.779462926474686	37.0	37.0	37.0	27.0	37.0
26	34.722180569714865	37.0	37.0	37.0	27.0	37.0
27	34.648826694584	37.0	37.0	37.0	27.0	37.0
28	34.618487160614606	37.0	37.0	37.0	27.0	37.0
29	34.65824879652192	37.0	37.0	37.0	27.0	37.0
30	34.62997106166654	37.0	37.0	37.0	27.0	37.0
31	34.63447181826909	37.0	37.0	37.0	27.0	37.0
32	34.64980273474109	37.0	37.0	37.0	27.0	37.0
33	34.62525772488925	37.0	37.0	37.0	27.0	37.0
34	34.53884959838348	37.0	37.0	37.0	27.0	37.0
35	34.52300208922881	37.0	37.0	37.0	27.0	37.0
36	34.501920078997536	37.0	37.0	37.0	27.0	37.0
37	34.525299326600866	37.0	37.0	37.0	27.0	37.0
38	34.37835613808111	37.0	37.0	37.0	27.0	37.0
39	34.43180290846252	37.0	37.0	37.0	27.0	37.0
40	34.47893399042704	37.0	37.0	37.0	27.0	37.0
41	34.47497954201544	37.0	37.0	37.0	27.0	37.0
42	34.38099167508606	37.0	37.0	37.0	27.0	37.0
43	34.110057099492096	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	10.0
16	27.0
17	25.0
18	26.0
19	22.0
20	71.0
21	187.0
22	477.0
23	1045.0
24	1884.0
25	3499.0
26	5394.0
27	7536.0
28	10469.0
29	13487.0
30	16912.0
31	20980.0
32	26012.0
33	32760.0
34	45266.0
35	77849.0
36	173542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.957570825771114	20.228717981539813	13.745022652360555	25.068688540328516
2	15.920197859569079	23.810579635276422	37.19215876310339	23.077063742051102
3	18.03662779268633	26.966595197059533	30.47668246922159	24.520094541032545
4	13.06636615906483	18.477331638787426	38.52204204973005	29.934260152417703
5	14.709176606123222	36.56836166973727	34.47593272408922	14.246529000050288
6	29.381323117293967	39.74952112315478	17.40803050182636	13.46112525772489
7	26.29136741625941	32.51082330244444	23.1250657169895	18.072743564306645
8	24.78090527153117	35.10178704495271	20.941204438125453	19.176103245390667
9	26.38714278530317	15.12130784809432	19.979564873526225	38.511984493076284
10	15.058905280674406	28.202760342139793	33.69532917925766	23.043005197928142
11	34.31226884763259	23.615143023027233	24.005787666692573	18.066800462647606
12	23.082778262877103	25.690428406197285	30.121925016343532	21.104868314582088
13	28.617863134940407	22.024677586735	26.10301680983446	23.254442468490133
14	22.32571854384866	21.05412336964721	27.84068830260445	28.779469783899682
15	24.08121934159577	28.54791740003017	24.279856085507518	23.09100717286654
16	22.815110107387277	28.194074270484272	25.265268056742908	23.725547565385547
17	22.437494571205217	27.230148897554642	26.51903392596724	23.8133226052729
18	23.762806241171067	26.366341929496528	27.569134272952944	22.301717556379465
19	24.938626046328764	26.224164651345657	27.21460540090792	21.62260390141766
20	24.673700860835417	25.93912435254479	27.163860455973044	22.223314330646744
21	23.458565152394844	26.726356741534506	27.177346725122405	22.637731380948246
22	23.1342089503111	26.635838731650672	27.303751925793517	22.92620039224471
23	23.008260911306067	26.54783511093028	27.62399367288254	22.819910304881116
24	23.180382278585178	26.715384861548593	26.983967340370572	23.120265519495657
25	23.341760346711407	26.54143484760516	27.81577299180309	22.301031813880343
26	23.334902921720207	26.656868168290355	27.10580092438089	22.90242798560855
27	23.329188400894207	26.620752396670035	27.261235890848077	22.788823311587677
28	22.91179979976319	26.463260202705484	27.42947138396551	23.195468613565815
29	23.226098445193173	26.65183938996347	27.10945821770953	23.012603947133826
30	23.47959458903452	26.561092799246598	27.363868684883037	22.59544392683585
31	23.5200533964826	26.307596655405252	27.213005335076645	22.95934461303551
32	22.440466122034735	26.554463955088437	27.66399531866454	23.341074604212288
33	22.71773467251224	26.44748812522572	27.81394434513877	23.020832857123263
34	23.1273515253199	26.194220562217417	27.36112571488655	23.31730219757613
35	22.967344942191907	26.635152989151557	27.514274873023346	22.88322719563319
36	23.405305818296522	26.335483517036128	27.22443437672864	23.034776287938705
37	23.28621520428269	26.209306897198054	27.33232452992352	23.172153368595737
38	22.992260252993265	26.608409031685877	27.213005335076645	23.186325380244217
39	22.686190517552724	26.279252632108292	27.715654586931578	23.31890226340741
40	22.797052221577115	26.435144760241563	27.910862618347725	22.85694039983359
41	23.013975432132064	26.14416135978166	27.458958311427672	23.382904896658605
42	22.72870655249816	26.55697834425188	27.553590776306226	23.160724326943736
43	22.877969836473273	26.060729355722064	27.68159604280862	23.379704764996045
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	18.5
2	33.0
3	121.5
4	210.0
5	210.0
6	270.5
7	331.0
8	337.5
9	344.0
10	445.0
11	546.0
12	546.0
13	842.5
14	1139.0
15	1992.5
16	2846.0
17	3155.5
18	3465.0
19	3465.0
20	3126.0
21	2787.0
22	3044.5
23	3302.0
24	4399.0
25	5496.0
26	5496.0
27	7088.5
28	8681.0
29	10589.5
30	12498.0
31	14568.0
32	16638.0
33	16638.0
34	19048.5
35	21459.0
36	23269.0
37	25079.0
38	26943.5
39	28808.0
40	28808.0
41	30712.0
42	32616.0
43	33413.0
44	34210.0
45	34263.0
46	34316.0
47	34316.0
48	34230.0
49	34144.0
50	33879.5
51	33615.0
52	32137.5
53	30660.0
54	30660.0
55	28528.0
56	26396.0
57	23793.0
58	21190.0
59	19582.5
60	17975.0
61	17975.0
62	15565.0
63	13155.0
64	10897.0
65	8639.0
66	7515.5
67	6392.0
68	6392.0
69	5254.0
70	4116.0
71	3400.5
72	2685.0
73	2208.0
74	1731.0
75	1731.0
76	1288.5
77	846.0
78	671.0
79	496.0
80	370.0
81	244.0
82	244.0
83	207.5
84	171.0
85	146.0
86	121.0
87	88.5
88	56.0
89	56.0
90	45.5
91	35.0
92	21.0
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	437482.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.50981788154391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.96177762665532	61.83716961247219
2	6.232126878008729	8.66388008610123
3	1.7133973237895106	3.5729380780600075
4	0.812320583970648	2.2585702341291656
5	0.47782184864519245	1.6606654839574981
6	0.31371208426198766	1.3083641906574188
7	0.23447007076128717	1.140858034210285
8	0.16282020867877192	0.9054082442157129
9	0.13413299924006644	0.8391204314174059
>10	0.8764422695789935	11.478932407701887
>50	0.055858367311860106	2.6547133462994332
>100	0.02412817044906716	2.6969865793494097
>500	3.305228828639337E-4	0.16449803141785307
>1k	6.610457657278674E-4	0.8178952400105544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2015	0.4605903785755757	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1545	0.35315738704678135	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.16366387645663136	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	449	0.1026327940349546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.571616660799759E-4	0.0	0.0
2	0.0	0.0	9.143233321599518E-4	0.0	0.0
3	0.0	0.0	0.0013714849982399276	0.0	0.0
4	0.0	0.0	0.0016000658312799155	0.0	0.0
5	0.0	0.0	0.003428712495599819	2.2858083303998794E-4	0.0
6	0.0	0.0	0.007314586657279614	2.2858083303998794E-4	0.0
7	0.0	0.0	0.014629173314559228	4.571616660799759E-4	0.0
8	0.0	0.0	0.01760072414407907	4.571616660799759E-4	0.0
9	0.0	0.0	0.021943759971838842	0.0011429041651999396	0.0
10	9.143233321599518E-4	0.0	0.024915310801358684	0.0011429041651999396	0.0
11	9.143233321599518E-4	0.0	0.036344352453358084	0.0011429041651999396	0.0
12	9.143233321599518E-4	0.0	0.04068738828111785	0.0011429041651999396	0.0
13	9.143233321599518E-4	0.0	0.04480184327583763	0.0011429041651999396	0.0
14	9.143233321599518E-4	0.0	0.049144879103597404	0.0011429041651999396	0.0
15	9.143233321599518E-4	0.0	0.05394507659743715	0.0011429041651999396	0.0
16	9.143233321599518E-4	0.0	0.059202435757356876	0.0011429041651999396	2.2858083303998794E-4
17	9.143233321599518E-4	0.0	0.06103108242167678	0.0013714849982399276	2.2858083303998794E-4
18	9.143233321599518E-4	0.0	0.062402567419916705	0.0013714849982399276	2.2858083303998794E-4
19	9.143233321599518E-4	0.0	0.06308830991903668	0.0013714849982399276	2.2858083303998794E-4
20	9.143233321599518E-4	0.0	0.06560269908247654	0.0013714849982399276	2.2858083303998794E-4
21	9.143233321599518E-4	0.0	0.0681170882459164	0.0016000658312799155	2.2858083303998794E-4
22	9.143233321599518E-4	0.0	0.06948857324415633	0.0016000658312799155	2.2858083303998794E-4
23	9.143233321599518E-4	0.0	0.07017431574327629	0.0016000658312799155	2.2858083303998794E-4
24	9.143233321599518E-4	0.0	0.07063147740935627	0.0020572274973598916	2.2858083303998794E-4
25	9.143233321599518E-4	0.0	0.07086005824239626	0.002285808330399879	2.2858083303998794E-4
26	9.143233321599518E-4	0.0	0.07086005824239626	0.002285808330399879	4.571616660799759E-4
27	9.143233321599518E-4	0.0	0.07086005824239626	0.003428712495599819	4.571616660799759E-4
28	9.143233321599518E-4	0.0	0.07086005824239626	0.011657622485039385	4.571616660799759E-4
29	9.143233321599518E-4	0.0	0.07086005824239626	0.03611577162031809	4.571616660799759E-4
30	9.143233321599518E-4	0.0	0.07086005824239626	0.06331689075207665	4.571616660799759E-4
31	9.143233321599518E-4	0.0	0.07086005824239626	0.15269199647071194	4.571616660799759E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	290	0.0	29.982758	1
GTGACGC	25	0.0054926695	29.599998	3
GTATCAA	1005	0.0	25.771143	1
TCGTATG	45	1.3213826E-4	24.666666	37
CCGGCAG	80	2.716115E-8	23.125002	16
GGACCGT	40	0.0019292855	23.125002	6
GTATAGA	95	7.1140676E-9	21.421053	1
ACGAGAC	95	7.1140676E-9	21.421053	22
ACCGTAC	80	6.939845E-7	20.8125	27
CGAACTA	45	0.0038220773	20.555555	29
CCTAGAG	45	0.0038220773	20.555555	2
GCCGGCA	90	9.444557E-8	20.555555	15
CATCGAC	45	0.0038220773	20.555555	15
TCCTAGA	65	6.8896465E-5	19.923077	1
GAGACCG	85	1.241704E-6	19.588236	24
TTCGCCG	95	1.670196E-7	19.473684	24
AGCTTCG	100	2.8648174E-7	18.499998	21
CGGCAGC	105	4.780486E-7	17.619047	17
CCGCTCT	105	4.780486E-7	17.619047	28
GCTTCGC	105	4.780486E-7	17.619047	22
>>END_MODULE