FastQCFastQC Report
Fri 10 Feb 2017
ERR1633009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515067
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25020.48576204649103905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.357429227653878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9480.18405372504936252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG250.005493562729.59999833
GCCGGCA1700.026.11764715
GCCGCTC1800.024.66666627
GGTATCA5350.024.5514011
AGCTTCG2000.024.0521
CCGCTCT1850.024.028
TATGTAC551.9000168E-523.5454543
GTATCAA14900.023.4664421
TTCTGCG400.001929748223.12518
TTCGCCG1850.023.024
CGGCAGC1950.022.76923217
CGCTCTC2050.021.65853729
GCTTCGC2050.021.65853722
TGCTCGC2050.021.65853710
CGCCGGC2050.021.65853714
CCGGCAG2050.020.756116
CGACGCT450.003822984920.55555514
CGAGTCG1001.2856617E-820.34999821
ATAATAC759.249499E-619.7333343
TTAACGG1052.2520908E-819.3809535
Produced by FastQC (version 0.11.5)