FastQCFastQC Report
Fri 10 Feb 2017
ERR1633008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285456
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5070.17761055994619135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.14222857463146685No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3070.10754722268931113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCA250.0054895129.633
ACCGAGA250.0054895129.628
GGTATCA1500.028.3666671
CATGCTA751.36460585E-824.6666664
ATAATAC502.6952132E-422.23
TTATACC450.003818871720.5555574
GTATCAA3350.019.3283581
CTAGAGG609.21365E-418.518
GTACATG1002.856159E-718.51
TTTAACG801.609744E-518.534
CATCCCG500.007022250418.536
TATACCA609.21365E-418.55
TATAATA1002.856159E-718.52
TACATGC953.5872126E-617.5263162
ACCACTC752.0604154E-417.26666630
AATAGTA650.001576003517.0769255
ATACACT650.001576003517.0769254
CTAATAC1201.033095E-716.9583323
TAATACA904.4271495E-516.4444454
TATGCTG803.371436E-416.18755
Produced by FastQC (version 0.11.5)