##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633008.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285456
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0655547615044	33.0	33.0	33.0	27.0	33.0
2	30.745071044223977	33.0	33.0	33.0	27.0	33.0
3	31.215343170225886	33.0	33.0	33.0	27.0	33.0
4	31.37509808867216	33.0	33.0	33.0	27.0	33.0
5	31.477253937559553	33.0	33.0	33.0	27.0	33.0
6	34.135173196569696	37.0	33.0	37.0	27.0	37.0
7	34.34815873549689	37.0	33.0	37.0	27.0	37.0
8	34.500353819853146	37.0	37.0	37.0	27.0	37.0
9	34.608289894064235	37.0	37.0	37.0	27.0	37.0
10	34.61027618967547	37.0	37.0	37.0	27.0	37.0
11	34.615646544476206	37.0	37.0	37.0	27.0	37.0
12	34.620708620593014	37.0	37.0	37.0	27.0	37.0
13	34.66485552939858	37.0	37.0	37.0	27.0	37.0
14	34.63198181155765	37.0	37.0	37.0	27.0	37.0
15	34.67920800403565	37.0	37.0	37.0	27.0	37.0
16	34.64349672103582	37.0	37.0	37.0	27.0	37.0
17	34.627953169665375	37.0	37.0	37.0	27.0	37.0
18	34.63156143153411	37.0	37.0	37.0	27.0	37.0
19	34.629592651757186	37.0	37.0	37.0	27.0	37.0
20	34.63564612409618	37.0	37.0	37.0	27.0	37.0
21	34.64766899276946	37.0	37.0	37.0	27.0	37.0
22	34.643437167199146	37.0	37.0	37.0	27.0	37.0
23	34.61971722437083	37.0	37.0	37.0	27.0	37.0
24	34.619177736673954	37.0	37.0	37.0	27.0	37.0
25	34.609484473964464	37.0	37.0	37.0	27.0	37.0
26	34.58367314051903	37.0	37.0	37.0	27.0	37.0
27	34.50650187769744	37.0	37.0	37.0	27.0	37.0
28	34.46465654952077	37.0	37.0	37.0	27.0	37.0
29	34.491827111708986	37.0	37.0	37.0	27.0	37.0
30	34.454136539431644	37.0	37.0	37.0	27.0	37.0
31	34.4659737402612	37.0	37.0	37.0	27.0	37.0
32	34.47790552659604	37.0	37.0	37.0	27.0	37.0
33	34.45813014965529	37.0	37.0	37.0	27.0	37.0
34	34.37127963679166	37.0	37.0	37.0	27.0	37.0
35	34.37843660669245	37.0	37.0	37.0	27.0	37.0
36	34.34934280589653	37.0	37.0	37.0	27.0	37.0
37	34.38905820862059	37.0	37.0	37.0	27.0	37.0
38	34.2581273471218	37.0	37.0	37.0	27.0	37.0
39	34.334478868897484	37.0	37.0	37.0	27.0	37.0
40	34.360665041197244	37.0	37.0	37.0	27.0	37.0
41	34.38085028866095	37.0	37.0	37.0	27.0	37.0
42	34.28865043999776	37.0	37.0	37.0	27.0	37.0
43	34.02762597388039	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	13.0
17	24.0
18	16.0
19	22.0
20	60.0
21	149.0
22	420.0
23	839.0
24	1521.0
25	2480.0
26	3878.0
27	5493.0
28	7236.0
29	9336.0
30	11728.0
31	14359.0
32	17773.0
33	22726.0
34	31092.0
35	52918.0
36	103360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.33616389215851	20.87537133568746	13.550950058853203	25.237514713300826
2	15.958676643685893	23.313925788913178	36.97207275376941	23.755324813631525
3	18.01608654223418	27.01186872933132	29.802841768959137	25.169202959475367
4	13.30958186200325	18.68624236309624	38.2657922762177	29.738383498682808
5	14.569320665881957	36.9633148366123	35.22399248921025	13.243372008295498
6	29.937713693178637	39.727313491396224	17.377809539824003	12.957163275601143
7	26.69167927806737	32.10547334790651	23.649529174373633	17.553318199652484
8	24.254526091586794	36.59793453281767	20.582856902639985	18.564682472955553
9	25.644232386077014	15.759696765876352	20.345692506025447	38.250378342021186
10	14.824351213497	28.36444145507539	34.203870298750076	22.607337032677542
11	34.25186368477103	23.580516787175608	24.183762120957343	17.983857407096014
12	22.989532537413822	25.43999775797321	30.45548175550698	21.11498794910599
13	27.745081553724564	22.2601731965697	26.16059918166022	23.834146068045513
14	21.799857070791994	20.78604058068494	27.421739252284066	29.992363096239
15	23.667045008687854	29.02829157558433	23.82188498402556	23.482778431702258
16	23.28625077069671	28.476893111372682	24.995796199764587	23.24105991816602
17	22.340045401042545	27.858584160080714	26.596743456084297	23.204626982792444
18	22.916666666666664	27.67606916652654	27.528235524914525	21.87902864189227
19	24.253825458214227	26.89135978924948	26.91378005717168	21.941034695364607
20	23.85376380247744	26.88400313883751	27.023078863292415	22.239154195392636
21	23.01405470545373	27.204542906787736	27.19543467294434	22.585967714814192
22	22.69421557087607	27.081932066588195	27.03604058068494	23.187811781850794
23	22.990583487472676	26.783462249873885	27.536643125385346	22.68931113726809
24	23.145773779496665	27.521229191188834	25.92133288492797	23.411664144386528
25	23.311473572109186	26.84757020346393	27.283364161201728	22.557592063225155
26	23.09287595986772	27.329255647104983	26.983493077742278	22.594375315285017
27	23.077462025671206	27.11626310184407	26.51652093492517	23.289753937559553
28	22.8304887618407	26.804831567737235	27.437853819853146	22.926825850568914
29	23.85376380247744	26.988747828036548	26.5315845524354	22.625903817050613
30	23.391696093268312	26.77785718289333	27.054257048371728	22.77618967546662
31	23.40080432711171	26.73441791379407	26.943206658819573	22.921571100274647
32	22.54428002914635	26.697284345047922	27.198937839807186	23.55949778599854
33	22.480872708928874	26.948811725800127	27.492503222913513	23.07781234235749
34	23.211983633204415	26.59499187265288	27.33065691385012	22.862367580292585
35	23.26418081946079	27.299478728770808	26.74737963118659	22.688960820581805
36	23.570007286587074	27.005913345664478	26.545246903200493	22.87883246454795
37	22.92016983352951	26.762443248696822	26.87454458830783	23.442842329465837
38	23.163990247183456	26.96422565999664	26.877347121798106	22.994436971021802
39	22.902653999215293	26.90572277338714	27.094893783980716	23.096729443416848
40	22.84835491284121	27.202791323356312	27.414732918558375	22.5341208452441
41	22.97341796984474	26.508113334454347	27.119065635334344	23.399403060366573
42	22.625903817050613	27.12922481923659	27.175816938512416	23.06905442520038
43	23.232652317695194	26.191777366739533	27.245529959083008	23.33004035648226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	34.0
4	63.0
5	63.0
6	73.5
7	84.0
8	81.0
9	78.0
10	111.5
11	145.0
12	145.0
13	208.0
14	271.0
15	545.0
16	819.0
17	966.0
18	1113.0
19	1113.0
20	1176.5
21	1240.0
22	1571.5
23	1903.0
24	2546.0
25	3189.0
26	3189.0
27	4399.0
28	5609.0
29	7008.0
30	8407.0
31	10153.0
32	11899.0
33	11899.0
34	13767.5
35	15636.0
36	17226.0
37	18816.0
38	20238.5
39	21661.0
40	21661.0
41	22742.0
42	23823.0
43	23935.5
44	24048.0
45	23462.5
46	22877.0
47	22877.0
48	21994.0
49	21111.0
50	20850.0
51	20589.0
52	19544.5
53	18500.0
54	18500.0
55	17433.0
56	16366.0
57	14437.5
58	12509.0
59	11603.0
60	10697.0
61	10697.0
62	9455.0
63	8213.0
64	6888.0
65	5563.0
66	4650.5
67	3738.0
68	3738.0
69	3150.5
70	2563.0
71	2104.5
72	1646.0
73	1325.0
74	1004.0
75	1004.0
76	757.0
77	510.0
78	416.5
79	323.0
80	250.0
81	177.0
82	177.0
83	136.0
84	95.0
85	98.0
86	101.0
87	70.5
88	40.0
89	40.0
90	31.0
91	22.0
92	12.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	285456.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.49074862852501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57264752560545	66.56251671924268
2	5.399052834318734	7.935608693580873
3	1.4932464671067178	3.2921940226371857
4	0.7108280428177347	2.089571400512982
5	0.409933982710172	1.5063177638821683
6	0.2628831590209846	1.1591688094970247
7	0.18145587134505234	0.9334729479732452
8	0.12350820492233781	0.7261368353206308
9	0.09166906724915162	0.606314554039688
>10	0.6760258530830329	9.471952992654238
>50	0.05474015285095477	2.738391556957791
>100	0.023528662190322527	2.7994407501734613
>500	4.8017677939433734E-4	0.17891295352804043
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.17761055994619135	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.14222857463146685	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	307	0.10754722268931113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.503166862844011E-4	0.0	0.0
2	0.0	0.0	3.503166862844011E-4	0.0	0.0
3	0.0	0.0	7.006333725688022E-4	0.0	0.0
4	0.0	0.0	7.006333725688022E-4	0.0	0.0
5	0.0	0.0	7.006333725688022E-4	0.0	0.0
6	0.0	0.0	0.0014012667451376044	0.0	0.0
7	0.0	0.0	0.003503166862844011	0.0	0.0
8	0.0	0.0	0.003503166862844011	0.0	0.0
9	0.0	0.0	0.005955383666834819	7.006333725688022E-4	0.0
10	0.0	0.0	0.005955383666834819	7.006333725688022E-4	0.0
11	0.0	0.0	0.010509500588532034	7.006333725688022E-4	0.0
12	0.0	0.0	0.011210133961100835	7.006333725688022E-4	0.0
13	0.0	0.0	0.011210133961100835	7.006333725688022E-4	0.0
14	0.0	0.0	0.011560450647385236	7.006333725688022E-4	0.0
15	0.0	0.0	0.011910767333669638	7.006333725688022E-4	0.0
16	0.0	0.0	0.011910767333669638	7.006333725688022E-4	0.0
17	0.0	0.0	0.012261084019954039	7.006333725688022E-4	0.0
18	0.0	0.0	0.01261140070623844	0.0010509500588532033	0.0
19	0.0	0.0	0.01261140070623844	0.0010509500588532033	0.0
20	3.503166862844011E-4	0.0	0.013312034078807242	0.0010509500588532033	0.0
21	3.503166862844011E-4	0.0	0.014362984137660444	0.0010509500588532033	0.0
22	3.503166862844011E-4	0.0	0.014362984137660444	0.0010509500588532033	0.0
23	3.503166862844011E-4	0.0	0.014713300823944847	0.0010509500588532033	0.0
24	3.503166862844011E-4	0.0	0.015413934196513648	0.0014012667451376044	0.0
25	3.503166862844011E-4	0.0	0.015413934196513648	0.0014012667451376044	0.0
26	3.503166862844011E-4	0.0	0.015413934196513648	0.0017515834314220055	0.0
27	3.503166862844011E-4	0.0	0.015413934196513648	0.0021019001177064065	0.0
28	3.503166862844011E-4	0.0	0.015413934196513648	0.007006333725688022	0.0
29	3.503166862844011E-4	0.0	0.015413934196513648	0.01611456756908245	0.0
30	3.503166862844011E-4	0.0	0.015413934196513648	0.031178185079311697	0.0
31	3.503166862844011E-4	0.0	0.015413934196513648	0.07706967098256824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCA	25	0.00548951	29.6	33
ACCGAGA	25	0.00548951	29.6	28
GGTATCA	150	0.0	28.366667	1
CATGCTA	75	1.36460585E-8	24.666666	4
ATAATAC	50	2.6952132E-4	22.2	3
TTATACC	45	0.0038188717	20.555557	4
GTATCAA	335	0.0	19.328358	1
CTAGAGG	60	9.21365E-4	18.5	18
GTACATG	100	2.856159E-7	18.5	1
TTTAACG	80	1.609744E-5	18.5	34
CATCCCG	50	0.0070222504	18.5	36
TATACCA	60	9.21365E-4	18.5	5
TATAATA	100	2.856159E-7	18.5	2
TACATGC	95	3.5872126E-6	17.526316	2
ACCACTC	75	2.0604154E-4	17.266666	30
AATAGTA	65	0.0015760035	17.076925	5
ATACACT	65	0.0015760035	17.076925	4
CTAATAC	120	1.033095E-7	16.958332	3
TAATACA	90	4.4271495E-5	16.444445	4
TATGCTG	80	3.371436E-4	16.1875	5
>>END_MODULE