FastQCFastQC Report
Fri 10 Feb 2017
ERR1633007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466151
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23510.5043430133154279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.36275799043657525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9390.20143687345945843No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4830.10361449401588756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG250.00549303429.59999810
GGTATCA5250.024.3142871
GGACCGT701.9178151E-723.7857156
TCTAGAC400.001929474923.1253
GTATCAA12450.021.8433741
ACCGTGG603.720173E-521.5833328
TAGGACC705.091193E-621.1428594
TACCGCA705.091193E-621.1428597
CCGTGTC555.1367923E-420.1818189
GCACCGT555.1367923E-420.1818186
GACCGTG656.890889E-519.9230757
CGAGTCG851.2420605E-619.58823621
CCGTCCG951.6707781E-719.47368428
GCGAGTC902.1466367E-618.520
CGTGTCT500.00702876418.49999810
CGCCGTC953.5970897E-617.52631626
GCCGTCC953.5970897E-617.52631627
ACGGGTA650.001578030417.07692116
TCGCCGT1005.8627265E-616.6525
GAGTCGC1005.8627265E-616.6522
Produced by FastQC (version 0.11.5)