##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633007.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466151
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64606747598954	33.0	33.0	33.0	27.0	33.0
2	31.181522725468785	33.0	33.0	33.0	27.0	33.0
3	31.622961229301236	33.0	33.0	33.0	27.0	33.0
4	31.756943565497018	33.0	33.0	33.0	27.0	33.0
5	31.866948692591027	33.0	33.0	33.0	33.0	33.0
6	34.73563501955375	37.0	37.0	37.0	27.0	37.0
7	34.875074814813225	37.0	37.0	37.0	27.0	37.0
8	35.079843226765576	37.0	37.0	37.0	33.0	37.0
9	35.14220928411609	37.0	37.0	37.0	33.0	37.0
10	35.19291173890006	37.0	37.0	37.0	33.0	37.0
11	35.23730936971067	37.0	37.0	37.0	33.0	37.0
12	35.1499599915049	37.0	37.0	37.0	33.0	37.0
13	35.20685357319839	37.0	37.0	37.0	33.0	37.0
14	35.149183419106684	37.0	37.0	37.0	33.0	37.0
15	35.215932176483584	37.0	37.0	37.0	33.0	37.0
16	35.19609954714245	37.0	37.0	37.0	33.0	37.0
17	35.17236260353405	37.0	37.0	37.0	33.0	37.0
18	35.125502251416385	37.0	37.0	37.0	33.0	37.0
19	35.16277343607543	37.0	37.0	37.0	33.0	37.0
20	35.11520515884338	37.0	37.0	37.0	33.0	37.0
21	35.17056061233377	37.0	37.0	37.0	33.0	37.0
22	35.17684827448616	37.0	37.0	37.0	33.0	37.0
23	35.1573674624746	37.0	37.0	37.0	33.0	37.0
24	35.158399316959525	37.0	37.0	37.0	33.0	37.0
25	35.14775469751218	37.0	37.0	37.0	33.0	37.0
26	35.11825567251813	37.0	37.0	37.0	33.0	37.0
27	35.05102638415449	37.0	37.0	37.0	27.0	37.0
28	35.03704379053139	37.0	37.0	37.0	27.0	37.0
29	35.07182865637959	37.0	37.0	37.0	33.0	37.0
30	35.04308260627994	37.0	37.0	37.0	27.0	37.0
31	35.0226407322949	37.0	37.0	37.0	27.0	37.0
32	35.05002885331148	37.0	37.0	37.0	27.0	37.0
33	35.01652254312444	37.0	37.0	37.0	27.0	37.0
34	34.943930185712354	37.0	37.0	37.0	27.0	37.0
35	34.955499398263655	37.0	37.0	37.0	27.0	37.0
36	34.92677694566782	37.0	37.0	37.0	27.0	37.0
37	34.94993038736375	37.0	37.0	37.0	27.0	37.0
38	34.811310069054876	37.0	37.0	37.0	27.0	37.0
39	34.861394698284464	37.0	37.0	37.0	27.0	37.0
40	34.891947030039624	37.0	37.0	37.0	27.0	37.0
41	34.89753105753286	37.0	37.0	37.0	27.0	37.0
42	34.77471892155117	37.0	37.0	37.0	27.0	37.0
43	34.573146898751695	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	21.0
17	27.0
18	22.0
19	18.0
20	33.0
21	114.0
22	298.0
23	643.0
24	1334.0
25	2486.0
26	4061.0
27	6218.0
28	8627.0
29	11697.0
30	15051.0
31	18965.0
32	24384.0
33	31997.0
34	44263.0
35	80285.0
36	215592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22033418355855	20.211691061480078	13.838863372598151	24.729111382363225
2	15.67689439687998	23.124052077545688	38.324705942924076	22.874347582650255
3	17.840570973783173	26.89514770964773	30.550401050303442	24.713880266265654
4	12.578756668976363	18.146694955068206	39.08797792989825	30.186570446057182
5	14.234657868373123	36.73380514039442	34.73938702266004	14.292149968572415
6	29.270129207059515	39.87806526211464	17.331722982467053	13.520082548358795
7	26.133591904769055	32.62762495414576	23.286231285570555	17.952551855514628
8	24.522525962617266	35.65454112508608	20.71667764308132	19.106255269215342
9	26.37986403547348	15.198937683282884	20.08726785955624	38.3339304216874
10	15.067864275738977	28.1460299345062	33.6105682493441	23.175537540410726
11	34.16553863447681	23.6095170878106	24.05937131959387	18.165572958118723
12	23.18497654193598	25.505683780577538	30.434129713333235	20.875209964153246
13	28.63170946753305	22.301786331038656	25.940306896263227	23.126197305165064
14	22.28505355560752	21.077504928660456	27.89353664370558	28.743904872026448
15	24.02612029149353	28.2485718147124	24.63343423053903	23.09187366325504
16	22.623570473945136	28.384364723018933	25.347580505029487	23.64448429800644
17	22.389740663433095	27.08843271815356	26.513297193398706	24.00852942501464
18	23.6408374110535	26.39766942471431	27.615944189758256	22.345548974473935
19	25.0206478158365	26.145819702199503	27.34736169181231	21.48617079015169
20	24.516733848044947	25.943524737692293	27.288367932279456	22.251373481983304
21	23.398855735587826	26.750130322577874	27.453335936209513	22.397678005624787
22	23.128557055546377	26.45988102567623	27.553089020510523	22.85847289826687
23	22.91339072532291	26.420194314717765	27.813519653502834	22.852895306456492
24	23.107748347638427	26.686846107806268	27.163730207593677	23.041675336961628
25	23.14228651231039	26.487768984728127	27.827678155790718	22.542266347170766
26	23.131345851451567	26.779519940963336	27.38554674343721	22.703587464147883
27	23.23839270965846	26.67912328837651	27.331272484666986	22.751211517298042
28	22.80806004921152	26.68555897123464	27.534425540221946	22.97195543933189
29	23.42416942149647	26.59631750226858	27.0060559775695	22.973457098665452
30	23.37075325377399	26.52080549006652	27.50267617145517	22.605765084704313
31	23.395852416920697	26.368494329090787	27.30681688980609	22.928836364182423
32	22.549774643838585	26.33889018794339	27.659063264907722	23.4522719033103
33	22.506012000403302	26.420623360241642	27.970764838003138	23.10259980135192
34	23.12169232716437	26.192371141539972	27.66228110633679	23.023655424958868
35	22.746492016535413	26.740262275528742	27.60543257442331	22.90781313351253
36	23.324845382719335	26.51651503482777	27.325480370094667	22.83315921235823
37	23.046394837724257	26.311216751653433	27.41515088458461	23.2272375260377
38	23.053045043344326	26.44872584205547	27.206634759981206	23.291594354618997
39	22.823934733594907	26.272817177266596	27.642330489476585	23.260917599661912
40	22.853753397504242	26.542901334546105	27.948668993523558	22.654676274426098
41	23.023011856673055	26.04477948132687	27.644046671572088	23.288161990427994
42	22.49421324849673	26.689420380949517	27.725779843870335	23.090586526683413
43	22.973457098665452	26.151397294009882	27.565531340702904	23.309614266621757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	21.0
2	38.0
3	134.5
4	231.0
5	231.0
6	277.5
7	324.0
8	329.5
9	335.0
10	442.0
11	549.0
12	549.0
13	831.5
14	1114.0
15	2035.5
16	2957.0
17	3365.0
18	3773.0
19	3773.0
20	3434.0
21	3095.0
22	3318.0
23	3541.0
24	4745.0
25	5949.0
26	5949.0
27	7625.5
28	9302.0
29	11440.0
30	13578.0
31	15900.5
32	18223.0
33	18223.0
34	20996.5
35	23770.0
36	25835.5
37	27901.0
38	29782.0
39	31663.0
40	31663.0
41	32975.5
42	34288.0
43	35044.0
44	35800.0
45	36113.0
46	36426.0
47	36426.0
48	36083.0
49	35740.0
50	35503.5
51	35267.0
52	33744.0
53	32221.0
54	32221.0
55	30153.5
56	28086.0
57	25165.5
58	22245.0
59	20520.0
60	18795.0
61	18795.0
62	16249.5
63	13704.0
64	11530.0
65	9356.0
66	8033.0
67	6710.0
68	6710.0
69	5602.0
70	4494.0
71	3626.5
72	2759.0
73	2278.0
74	1797.0
75	1797.0
76	1349.5
77	902.0
78	703.0
79	504.0
80	390.0
81	276.0
82	276.0
83	234.0
84	192.0
85	162.0
86	132.0
87	102.0
88	72.0
89	72.0
90	51.0
91	30.0
92	19.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466151.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.33036358818265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.96368474884632	65.07106027616695
2	6.063211317094043	8.771085581547919
3	1.5099501480729831	3.2764572963044727
4	0.6751802447560227	1.9534413036304505
5	0.3983888601063323	1.4407805550486321
6	0.24746252497271398	1.0739432643435678
7	0.19315071051599897	0.9779462783256612
8	0.13306423558473454	0.7699667632341952
9	0.09846146797496802	0.6409578400250073
>10	0.6330645736873306	8.750626136190162
>50	0.0533718347939472	2.6992636283682594
>100	0.03011483358708969	3.5002435329170183
>500	2.981666691791054E-4	0.2025094687251434
>1k	5.963333383582108E-4	0.8717180751725555
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2351	0.5043430133154279	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1691	0.36275799043657525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	939	0.20143687345945843	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	483	0.10361449401588756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.290455238753108E-4	0.0	0.0
2	4.290455238753108E-4	0.0	4.290455238753108E-4	0.0	0.0
3	4.290455238753108E-4	0.0	0.001072613809688277	0.0	0.0
4	4.290455238753108E-4	0.0	0.0012871365716259323	4.290455238753108E-4	0.0
5	4.290455238753108E-4	0.0	0.0015016593335635878	4.290455238753108E-4	0.0
6	4.290455238753108E-4	0.0	0.004290455238753108	4.290455238753108E-4	0.0
7	4.290455238753108E-4	0.0	0.009439001525256837	4.290455238753108E-4	0.0
8	4.290455238753108E-4	0.0	0.012656842954321668	4.290455238753108E-4	0.0
9	4.290455238753108E-4	0.0	0.01437302504982291	0.0012871365716259323	0.0
10	0.0015016593335635878	0.0	0.018019912002763053	0.0012871365716259323	0.0
11	0.0015016593335635878	0.0	0.02938961838545879	0.0012871365716259323	0.0
12	0.0015016593335635878	0.0	0.031534846004835346	0.0012871365716259323	0.0
13	0.0015016593335635878	0.0	0.03475268743390018	0.0012871365716259323	0.0
14	0.0015016593335635878	0.0	0.03646886952940142	0.0015016593335635878	0.0
15	0.0015016593335635878	0.0	0.04118837029202983	0.0015016593335635878	0.0
16	0.0015016593335635878	0.0	0.044406211721094664	0.0015016593335635878	0.0
17	0.0015016593335635878	0.0	0.045478825530782946	0.001716182095501243	0.0
18	0.0015016593335635878	0.0	0.046765962102408874	0.001716182095501243	0.0
19	0.0015016593335635878	0.0	0.047838575912097156	0.001716182095501243	0.0
20	0.0015016593335635878	0.0	0.04869666695984778	0.001716182095501243	0.0
21	0.0015016593335635878	0.0	0.05084189457922433	0.001716182095501243	2.145227619376554E-4
22	0.0015016593335635878	0.0	0.05127094010309964	0.002145227619376554	2.145227619376554E-4
23	0.0015016593335635878	0.0	0.051485462865037294	0.002145227619376554	2.145227619376554E-4
24	0.0015016593335635878	0.0	0.05255807667472557	0.003217841429064831	2.145227619376554E-4
25	0.0015016593335635878	0.0	0.05255807667472557	0.003217841429064831	2.145227619376554E-4
26	0.0015016593335635878	0.0	0.05255807667472557	0.0038614097148777973	2.145227619376554E-4
27	0.0015016593335635878	0.0	0.05277259943666323	0.005363069048441385	2.145227619376554E-4
28	0.0015016593335635878	0.0	0.05277259943666323	0.0139439795259476	2.145227619376554E-4
29	0.0015016593335635878	0.0	0.05277259943666323	0.031534846004835346	2.145227619376554E-4
30	0.0015016593335635878	0.0	0.05277259943666323	0.059422805056730546	2.145227619376554E-4
31	0.0015016593335635878	0.0	0.05277259943666323	0.133218635163284	2.145227619376554E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGCG	25	0.005493034	29.599998	10
GGTATCA	525	0.0	24.314287	1
GGACCGT	70	1.9178151E-7	23.785715	6
TCTAGAC	40	0.0019294749	23.125	3
GTATCAA	1245	0.0	21.843374	1
ACCGTGG	60	3.720173E-5	21.583332	8
TAGGACC	70	5.091193E-6	21.142859	4
TACCGCA	70	5.091193E-6	21.142859	7
CCGTGTC	55	5.1367923E-4	20.181818	9
GCACCGT	55	5.1367923E-4	20.181818	6
GACCGTG	65	6.890889E-5	19.923075	7
CGAGTCG	85	1.2420605E-6	19.588236	21
CCGTCCG	95	1.6707781E-7	19.473684	28
GCGAGTC	90	2.1466367E-6	18.5	20
CGTGTCT	50	0.007028764	18.499998	10
CGCCGTC	95	3.5970897E-6	17.526316	26
GCCGTCC	95	3.5970897E-6	17.526316	27
ACGGGTA	65	0.0015780304	17.076921	16
TCGCCGT	100	5.8627265E-6	16.65	25
GAGTCGC	100	5.8627265E-6	16.65	22
>>END_MODULE