FastQCFastQC Report
Fri 10 Feb 2017
ERR1633006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493000
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16960.3440162271805274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.23894523326572006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6160.12494929006085193No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5820.118052738336714No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5140.10425963488843812No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5080.10304259634888438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA358.8621816E-426.4285721
GGTATCA4050.023.7530861
GTATCAA10200.022.127451
TTAACGG1107.4578566E-1121.86363635
TTTAACG1151.3460522E-1020.91304434
CGCATCT450.003822755520.55555512
TATTGCG450.003822755520.55555511
GGTATAT450.003822755520.5555551
GCATAGG450.003822755520.5555551
GCCGGCA1202.382876E-1020.04166615
AACGGCC1202.382876E-1020.04166637
TAGGACC1500.019.7333344
CCGGCAG1355.638867E-1119.18518616
TGCTCGC1355.638867E-1119.18518610
TAACGGC1306.9303496E-1018.50000236
GCACCGC1306.9303496E-1018.50000210
TTGCCGA609.2266215E-418.510
CGGAGAG609.2266215E-418.537
GCTTCGC1351.1459633E-917.81481622
TTCGCCG1258.543793E-917.7624
Produced by FastQC (version 0.11.5)