##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633006.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493000
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67688640973631	33.0	33.0	33.0	27.0	33.0
2	31.193387423935093	33.0	33.0	33.0	27.0	33.0
3	31.63727789046653	33.0	33.0	33.0	27.0	33.0
4	31.7739046653144	33.0	33.0	33.0	27.0	33.0
5	31.885077079107504	33.0	33.0	33.0	33.0	33.0
6	34.76661054766734	37.0	37.0	37.0	27.0	37.0
7	34.938720081135905	37.0	37.0	37.0	27.0	37.0
8	35.122223123732255	37.0	37.0	37.0	33.0	37.0
9	35.231912778904665	37.0	37.0	37.0	33.0	37.0
10	35.25160040567951	37.0	37.0	37.0	33.0	37.0
11	35.28904462474645	37.0	37.0	37.0	33.0	37.0
12	35.22534888438134	37.0	37.0	37.0	33.0	37.0
13	35.264490872210956	37.0	37.0	37.0	33.0	37.0
14	35.24313184584179	37.0	37.0	37.0	33.0	37.0
15	35.264659229208924	37.0	37.0	37.0	33.0	37.0
16	35.25326572008114	37.0	37.0	37.0	33.0	37.0
17	35.20602434077079	37.0	37.0	37.0	33.0	37.0
18	35.22813995943205	37.0	37.0	37.0	33.0	37.0
19	35.23838336713996	37.0	37.0	37.0	33.0	37.0
20	35.237409736308315	37.0	37.0	37.0	33.0	37.0
21	35.23917444219067	37.0	37.0	37.0	33.0	37.0
22	35.238040567951316	37.0	37.0	37.0	33.0	37.0
23	35.23624543610548	37.0	37.0	37.0	33.0	37.0
24	35.2240770791075	37.0	37.0	37.0	33.0	37.0
25	35.22916430020284	37.0	37.0	37.0	33.0	37.0
26	35.18601014198783	37.0	37.0	37.0	33.0	37.0
27	35.130245436105476	37.0	37.0	37.0	33.0	37.0
28	35.09449695740365	37.0	37.0	37.0	33.0	37.0
29	35.13202231237322	37.0	37.0	37.0	33.0	37.0
30	35.08944827586207	37.0	37.0	37.0	33.0	37.0
31	35.08249492900609	37.0	37.0	37.0	33.0	37.0
32	35.105724137931034	37.0	37.0	37.0	33.0	37.0
33	35.08966734279919	37.0	37.0	37.0	33.0	37.0
34	35.0429107505071	37.0	37.0	37.0	27.0	37.0
35	35.0348093306288	37.0	37.0	37.0	27.0	37.0
36	35.02563286004057	37.0	37.0	37.0	27.0	37.0
37	35.026101419878295	37.0	37.0	37.0	27.0	37.0
38	34.88071602434077	37.0	37.0	37.0	27.0	37.0
39	34.956703853955375	37.0	37.0	37.0	27.0	37.0
40	34.99132860040568	37.0	37.0	37.0	27.0	37.0
41	35.00630425963489	37.0	37.0	37.0	27.0	37.0
42	34.920638945233264	37.0	37.0	37.0	27.0	37.0
43	34.69067342799189	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	10.0
16	31.0
17	34.0
18	26.0
19	28.0
20	40.0
21	105.0
22	269.0
23	610.0
24	1256.0
25	2466.0
26	3898.0
27	6063.0
28	8732.0
29	11709.0
30	15345.0
31	19623.0
32	25234.0
33	32653.0
34	46947.0
35	83800.0
36	234120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91156186612576	20.254158215010143	13.491480730223124	25.342799188640974
2	15.931237322515212	22.76977687626775	37.69553752535497	23.603448275862068
3	17.43711967545639	26.71075050709939	30.389046653144014	25.463083164300205
4	12.730425963488845	17.81764705882353	39.11967545638945	30.332251521298176
5	14.037728194726165	36.816430020283974	35.35679513184584	13.789046653144016
6	29.824543610547664	39.774645030425965	17.19188640973631	13.20892494929006
7	26.756997971602438	32.130628803245436	23.576876267748478	17.53549695740365
8	24.619066937119673	35.744624746450306	20.50243407707911	19.133874239350913
9	26.055780933062877	15.34705882352941	20.0868154158215	38.51034482758621
10	15.095943204868153	27.807302231237323	33.8342799188641	23.262474645030427
11	34.40588235294118	23.238336713995945	24.066937119675455	18.288843813387423
12	23.270993914807303	25.563894523326574	30.069776876267753	21.095334685598377
13	28.006693711967547	22.097768762677486	25.9131845841785	23.982352941176472
14	22.137728194726165	20.784584178498985	27.506288032454364	29.571399594320486
15	24.146450304259634	28.37444219066937	24.36551724137931	23.113590263691684
16	23.129614604462475	28.248073022312376	24.963488843813387	23.658823529411766
17	22.571196754563893	27.19716024340771	26.362677484787017	23.86896551724138
18	23.20689655172414	27.003042596348887	27.64401622718053	22.146044624746448
19	24.851724137931033	26.204259634888437	26.987423935091275	21.95659229208925
20	24.249898580121705	26.270182555780934	27.19918864097363	22.280730223123733
21	23.24868154158215	27.063691683569978	27.13975659229209	22.547870182555783
22	22.96206896551724	26.639553752535498	27.178296146044623	23.22008113590264
23	22.897160243407708	26.56977687626775	27.67687626774848	22.856186612576064
24	23.30993914807302	27.05679513184584	26.227789046653143	23.40547667342799
25	23.392697768762677	26.721501014198783	27.37200811359026	22.513793103448275
26	23.311156186612575	27.03184584178499	27.082352941176467	22.574645030425962
27	23.21866125760649	26.828397565922923	26.704259634888437	23.24868154158215
28	22.82657200811359	26.51866125760649	27.516227180527387	23.138539553752537
29	24.06450304259635	26.551521298174443	26.659229208924952	22.72474645030426
30	23.72860040567951	26.257809330628803	27.072819472616633	22.940770791075053
31	23.358823529411765	26.534685598377283	27.167545638945235	22.93894523326572
32	22.503245436105477	26.399594320486813	27.427383367139957	23.669776876267747
33	22.48397565922921	26.594320486815416	27.922109533468557	22.999594320486814
34	23.20892494929006	26.19229208924949	27.49817444219067	23.100608519269777
35	22.85070993914807	27.066328600405683	27.233265720081135	22.84969574036511
36	23.884381338742394	26.568356997971605	26.789046653144016	22.75821501014199
37	23.04888438133874	26.40081135902637	26.952738336713995	23.597565922920893
38	23.17505070993915	26.587423935091277	27.041379310344826	23.196146044624747
39	22.66916835699797	26.46632860040568	27.341582150101416	23.522920892494927
40	22.881947261663285	27.046247464503043	27.547870182555783	22.52393509127789
41	23.00973630831643	26.13610547667343	27.347464503042595	23.506693711967547
42	22.606896551724137	27.066937119675455	27.20973630831643	23.116430020283975
43	23.38498985801217	25.779107505070993	27.388843813387425	23.447058823529414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	14.0
2	20.0
3	100.0
4	180.0
5	180.0
6	211.5
7	243.0
8	236.0
9	229.0
10	314.5
11	400.0
12	400.0
13	612.0
14	824.0
15	1506.5
16	2189.0
17	2524.0
18	2859.0
19	2859.0
20	2667.5
21	2476.0
22	2713.5
23	2951.0
24	4151.0
25	5351.0
26	5351.0
27	7110.0
28	8869.0
29	11032.0
30	13195.0
31	16448.0
32	19701.0
33	19701.0
34	22776.0
35	25851.0
36	28189.5
37	30528.0
38	33255.0
39	35982.0
40	35982.0
41	37653.5
42	39325.0
43	40058.0
44	40791.0
45	39945.0
46	39099.0
47	39099.0
48	37736.0
49	36373.0
50	36686.5
51	37000.0
52	35168.5
53	33337.0
54	33337.0
55	31737.0
56	30137.0
57	26474.5
58	22812.0
59	21130.5
60	19449.0
61	19449.0
62	16954.0
63	14459.0
64	12159.0
65	9859.0
66	8297.0
67	6735.0
68	6735.0
69	5663.5
70	4592.0
71	3739.5
72	2887.0
73	2391.0
74	1895.0
75	1895.0
76	1447.0
77	999.0
78	785.0
79	571.0
80	451.5
81	332.0
82	332.0
83	276.5
84	221.0
85	188.0
86	155.0
87	115.5
88	76.0
89	76.0
90	52.0
91	28.0
92	19.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	493000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.82688374119132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03071410653823	58.60614466886334
2	6.2368369946005	8.211030875126564
3	1.6784725207949676	3.3146584646746398
4	0.7866624521214193	2.071341511174286
5	0.47270636412410905	1.555839343745949
6	0.3123881118484409	1.2338121552466554
7	0.23312410100224012	1.0742083165760943
8	0.15251754497716585	0.8031803761363051
9	0.11986615635781442	0.710137398516238
>10	0.8254599998107394	10.396448083878896
>50	0.09391239646024661	4.317171017206144
>100	0.05547959996855707	6.666723961573269
>500	0.001239767597062727	0.45293570800259064
>1k	6.198837985313635E-4	0.5863681192790295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1696	0.3440162271805274	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1178	0.23894523326572006	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	616	0.12494929006085193	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	582	0.118052738336714	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	514	0.10425963488843812	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	508	0.10304259634888438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.0283975659229209E-4	0.0	0.0
2	0.0	0.0	2.0283975659229209E-4	0.0	0.0
3	0.0	0.0	6.085192697768763E-4	0.0	0.0
4	0.0	0.0	6.085192697768763E-4	0.0	0.0
5	0.0	0.0	0.0010141987829614604	0.0	0.0
6	0.0	0.0	0.002434077079107505	0.0	0.0
7	0.0	0.0	0.006085192697768763	0.0	0.0
8	0.0	0.0	0.007505070993914807	0.0	0.0
9	0.0	0.0	0.008924949290060852	6.085192697768763E-4	0.0
10	4.0567951318458417E-4	0.0	0.009330628803245436	6.085192697768763E-4	0.0
11	6.085192697768763E-4	0.0	0.013184584178498986	6.085192697768763E-4	0.0
12	6.085192697768763E-4	0.0	0.01460446247464503	6.085192697768763E-4	0.0
13	6.085192697768763E-4	0.0	0.01561866125760649	6.085192697768763E-4	0.0
14	6.085192697768763E-4	0.0	0.01663286004056795	6.085192697768763E-4	0.0
15	6.085192697768763E-4	0.0	0.018052738336713996	6.085192697768763E-4	0.0
16	6.085192697768763E-4	0.0	0.020081135902636917	6.085192697768763E-4	0.0
17	6.085192697768763E-4	0.0	0.0204868154158215	6.085192697768763E-4	0.0
18	6.085192697768763E-4	0.0	0.021095334685598377	6.085192697768763E-4	0.0
19	6.085192697768763E-4	0.0	0.021906693711967545	6.085192697768763E-4	0.0
20	6.085192697768763E-4	0.0	0.023123732251521298	6.085192697768763E-4	0.0
21	6.085192697768763E-4	0.0	0.02332657200811359	6.085192697768763E-4	0.0
22	6.085192697768763E-4	0.0	0.023529411764705882	0.0010141987829614604	0.0
23	6.085192697768763E-4	0.0	0.023732251521298174	0.0014198782961460446	0.0
24	6.085192697768763E-4	0.0	0.023732251521298174	0.0016227180527383367	0.0
25	6.085192697768763E-4	0.0	0.023732251521298174	0.0016227180527383367	0.0
26	6.085192697768763E-4	0.0	0.023732251521298174	0.002028397565922921	0.0
27	6.085192697768763E-4	0.0	0.023732251521298174	0.0032454361054766734	0.0
28	6.085192697768763E-4	0.0	0.023732251521298174	0.007910750507099391	0.0
29	6.085192697768763E-4	0.0	0.023732251521298174	0.02434077079107505	0.0
30	8.113590263691683E-4	0.0	0.023732251521298174	0.043204868154158214	0.0
31	8.113590263691683E-4	0.0	0.023935091277890466	0.11379310344827587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	35	8.8621816E-4	26.428572	1
GGTATCA	405	0.0	23.753086	1
GTATCAA	1020	0.0	22.12745	1
TTAACGG	110	7.4578566E-11	21.863636	35
TTTAACG	115	1.3460522E-10	20.913044	34
CGCATCT	45	0.0038227555	20.555555	12
TATTGCG	45	0.0038227555	20.555555	11
GGTATAT	45	0.0038227555	20.555555	1
GCATAGG	45	0.0038227555	20.555555	1
GCCGGCA	120	2.382876E-10	20.041666	15
AACGGCC	120	2.382876E-10	20.041666	37
TAGGACC	150	0.0	19.733334	4
CCGGCAG	135	5.638867E-11	19.185186	16
TGCTCGC	135	5.638867E-11	19.185186	10
TAACGGC	130	6.9303496E-10	18.500002	36
GCACCGC	130	6.9303496E-10	18.500002	10
TTGCCGA	60	9.2266215E-4	18.5	10
CGGAGAG	60	9.2266215E-4	18.5	37
GCTTCGC	135	1.1459633E-9	17.814816	22
TTCGCCG	125	8.543793E-9	17.76	24
>>END_MODULE