FastQCFastQC Report
Fri 10 Feb 2017
ERR1633005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458224
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16480.3596494291001781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.23852962743112538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6100.13312266489751737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG200.001840781437.011
ATCTCCG250.005492936429.610
AGACGCG250.005492936429.625
GACGCGT250.005492936429.626
CGCGTAG250.005492936429.628
TATCGCC250.005492936429.633
TCTATCG250.005492936429.631
ACGCGTA250.005492936429.627
ATCTCGG551.8994226E-523.54545412
GTACTAA400.00192942523.1251
TATAAGG400.00192942523.1252
GGTATCA3450.023.0579721
GTATCAA10050.022.8258721
CGCGGTA502.6986666E-422.212
GCCGCTC753.73062E-722.227
CGCCGTC753.73062E-722.226
TTCGCCG753.73062E-722.224
ACGGGTA450.0038223520.55555516
GCGGTAC555.136607E-420.18181813
TAGGTGC555.136607E-420.1818186
Produced by FastQC (version 0.11.5)