FastQCFastQC Report
Fri 10 Feb 2017
ERR1633004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480681
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21240.4418730925499448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.2920856035499635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7820.162685856108313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT358.861856E-426.4285726
GGTATCA4200.025.1071451
TCGTTTG451.3216477E-424.66666612
TGTACTA551.8996743E-523.5454545
GTATCAA11600.022.6465511
CGGGGAA656.8914575E-519.92307717
GTTCGTT500.007029073318.510
TTAAGAC500.007029073318.53
TTAACGG701.21732504E-418.535
TTGTACT1054.7829235E-717.619054
TACCGCA953.5975627E-617.5263167
TAGTACC852.7177823E-517.4117644
AACGGCC752.0641153E-417.26666637
TTTAGAC650.001578126617.0769233
CTCTATA650.001578126617.0769232
ACCGACC1107.7843833E-716.8181828
GTGTATA1005.8634887E-616.6500021
ATAGCCT803.3774454E-416.18754
GCCGGCA803.3774454E-416.187515
TTCGCCG803.3774454E-416.187524
Produced by FastQC (version 0.11.5)