##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633004.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480681
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.725930502765866	33.0	33.0	33.0	27.0	33.0
2	31.23687851194451	33.0	33.0	33.0	27.0	33.0
3	31.681420734333166	33.0	33.0	33.0	27.0	33.0
4	31.800917864446482	33.0	33.0	33.0	27.0	33.0
5	31.92534341902426	33.0	33.0	33.0	33.0	33.0
6	34.821093407062065	37.0	37.0	37.0	27.0	37.0
7	35.00100690478717	37.0	37.0	37.0	33.0	37.0
8	35.17698223978064	37.0	37.0	37.0	33.0	37.0
9	35.26626598513359	37.0	37.0	37.0	33.0	37.0
10	35.290279832154795	37.0	37.0	37.0	33.0	37.0
11	35.32832793474258	37.0	37.0	37.0	33.0	37.0
12	35.27288159923109	37.0	37.0	37.0	33.0	37.0
13	35.31506134005713	37.0	37.0	37.0	33.0	37.0
14	35.271847649480634	37.0	37.0	37.0	33.0	37.0
15	35.292383098146175	37.0	37.0	37.0	33.0	37.0
16	35.300650535386254	37.0	37.0	37.0	33.0	37.0
17	35.25312005259205	37.0	37.0	37.0	33.0	37.0
18	35.26205737276905	37.0	37.0	37.0	33.0	37.0
19	35.26530900950942	37.0	37.0	37.0	33.0	37.0
20	35.27159800366563	37.0	37.0	37.0	33.0	37.0
21	35.27681768158092	37.0	37.0	37.0	33.0	37.0
22	35.27114448043505	37.0	37.0	37.0	33.0	37.0
23	35.271537672593674	37.0	37.0	37.0	33.0	37.0
24	35.273166611536546	37.0	37.0	37.0	33.0	37.0
25	35.256190696116555	37.0	37.0	37.0	33.0	37.0
26	35.21996292759647	37.0	37.0	37.0	33.0	37.0
27	35.182703289707725	37.0	37.0	37.0	33.0	37.0
28	35.14486946644448	37.0	37.0	37.0	33.0	37.0
29	35.17764380119039	37.0	37.0	37.0	33.0	37.0
30	35.14392913387465	37.0	37.0	37.0	33.0	37.0
31	35.12302337725019	37.0	37.0	37.0	33.0	37.0
32	35.14592422001286	37.0	37.0	37.0	33.0	37.0
33	35.13575531381519	37.0	37.0	37.0	33.0	37.0
34	35.07567388767187	37.0	37.0	37.0	33.0	37.0
35	35.062742234454866	37.0	37.0	37.0	27.0	37.0
36	35.058985064939115	37.0	37.0	37.0	27.0	37.0
37	35.08145110790732	37.0	37.0	37.0	33.0	37.0
38	34.915727894383174	37.0	37.0	37.0	27.0	37.0
39	34.99118334196692	37.0	37.0	37.0	27.0	37.0
40	35.03094359876925	37.0	37.0	37.0	27.0	37.0
41	35.032168943644535	37.0	37.0	37.0	27.0	37.0
42	34.95195774328505	37.0	37.0	37.0	27.0	37.0
43	34.72475716743537	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	17.0
16	24.0
17	18.0
18	25.0
19	15.0
20	34.0
21	105.0
22	245.0
23	575.0
24	1254.0
25	2235.0
26	3711.0
27	5741.0
28	8070.0
29	11136.0
30	14785.0
31	18887.0
32	23921.0
33	31435.0
34	44770.0
35	81612.0
36	232066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36818388910733	19.88928208104751	13.74008958123995	25.00244444860521
2	15.89141239200218	22.88503186104714	37.96842396516609	23.255131781784595
3	17.910006844456095	26.663213232892502	30.194661324246226	25.23211859840518
4	12.718414083352577	17.923529326101924	38.86423636465764	30.49382022588786
5	14.380847173073203	36.32325804431629	34.94209257282896	14.353802209781538
6	29.73364871921295	39.472331962361736	17.249693663781176	13.54432565464414
7	26.805095271084152	32.07553450209183	23.15568953214294	17.963680694681088
8	24.785876704092736	35.35712874026641	20.526503023834934	19.33049153180592
9	26.384025996450866	15.150172359631439	19.811059725680856	38.65474191823684
10	15.292262436002254	27.79390073666319	33.44879452277082	23.46504230456373
11	34.481079967795694	23.335642557122082	23.782716604151194	18.40056087093103
12	23.505817787680392	25.374624751134327	29.984958839646254	21.134598621539023
13	28.55823300692143	21.95239670384309	25.77280982605928	23.716560463176204
14	22.46375454823469	20.679203047343247	27.547167456171557	29.309874948250503
15	24.33297758804696	28.053740422442324	24.225421849417806	23.387860140092908
16	23.092862002034614	27.992993274125666	25.097517896484362	23.816626827355357
17	22.84092776706381	26.83962960882581	26.060942704205075	24.2584999199053
18	23.91107616069701	26.538182287213353	27.125474067000777	22.425267485088863
19	25.325319702671834	25.94922620199259	26.85211189957581	21.873342195759765
20	24.665214560176082	25.81233708010094	27.048916017067455	22.473532342655524
21	23.657061543934542	26.543383241692514	26.9313744458383	22.86818076853464
22	23.257420201755426	26.36530256032587	27.084906622063283	23.292370615855422
23	23.19854539705127	26.216763300400892	27.500358865859063	23.084332436688783
24	23.53473509458456	26.55024850160502	26.47493868074669	23.440077723063737
25	23.540560163601224	26.305179526546713	27.483091696988232	22.67116861286383
26	23.55200226345539	26.419184448730032	27.006476228517457	23.022337059297122
27	23.558451447009553	26.37840896561337	26.862513808534143	23.200625778842934
28	23.11408189630961	26.4031655089342	27.207233071413267	23.275519523342926
29	24.015511326638663	26.39734043991753	26.524451767388353	23.062696466055453
30	23.85344958506785	26.1218978907009	26.99669843409663	23.02795409013462
31	23.654565085784544	26.206361391442556	26.915147467863303	23.223926054909597
32	22.677617796417998	26.110455790846736	27.214722445863266	23.997203966872
33	22.757296419038823	26.201992589680057	27.7246240230007	23.31608696828042
34	23.346044466080414	25.91885262783426	27.353691949546583	23.381410956538744
35	23.15568953214294	26.543799318050848	27.236358416496596	23.06415273330962
36	23.85969073044285	26.23028578204672	26.781378918659154	23.128644568851275
37	23.204162427888768	26.220299949446723	27.020206748342456	23.555330874322056
38	23.418025676072073	26.443108839334194	26.701908334217496	23.436957150376237
39	23.061032160622116	26.145614243125898	27.09468441648411	23.69866917976787
40	23.14071078324294	26.558778066950843	27.283375044988254	23.017136104817958
41	23.287585737734588	25.80359947657594	27.30646728287575	23.602347502813718
42	22.89668199908047	26.681936669017496	27.309379817384087	23.112001514517942
43	23.40824788165124	25.78154742958428	27.162088786534106	23.648115902230376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	17.0
2	33.0
3	106.5
4	180.0
5	180.0
6	241.0
7	302.0
8	292.0
9	282.0
10	356.0
11	430.0
12	430.0
13	753.0
14	1076.0
15	1845.0
16	2614.0
17	3008.5
18	3403.0
19	3403.0
20	2980.0
21	2557.0
22	2725.5
23	2894.0
24	3933.0
25	4972.0
26	4972.0
27	6612.5
28	8253.0
29	10327.0
30	12401.0
31	15058.5
32	17716.0
33	17716.0
34	20490.0
35	23264.0
36	25709.0
37	28154.0
38	30357.5
39	32561.0
40	32561.0
41	34195.5
42	35830.0
43	37001.5
44	38173.0
45	38186.0
46	38199.0
47	38199.0
48	37435.5
49	36672.0
50	36861.0
51	37050.0
52	35632.5
53	34215.0
54	34215.0
55	32323.5
56	30432.0
57	27258.0
58	24084.0
59	22182.5
60	20281.0
61	20281.0
62	17644.5
63	15008.0
64	12516.0
65	10024.0
66	8681.5
67	7339.0
68	7339.0
69	6078.0
70	4817.0
71	3912.0
72	3007.0
73	2499.0
74	1991.0
75	1991.0
76	1511.5
77	1032.0
78	821.5
79	611.0
80	487.5
81	364.0
82	364.0
83	291.5
84	219.0
85	177.0
86	135.0
87	100.5
88	66.0
89	66.0
90	47.5
91	29.0
92	17.5
93	6.0
94	4.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	480681.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.44264575379044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.06302248416644	60.957088976456774
2	6.4262973396627965	8.796655846541334
3	1.693199402919081	3.4766114077356045
4	0.738929204106838	2.0229707901525855
5	0.4592736783235075	1.5716952834768063
6	0.2959710294518768	1.2154224193295686
7	0.19593216483556794	0.9387081024729839
8	0.16933666609525178	0.9271879560548171
9	0.11255724218207318	0.693334390832211
>10	0.721504696245863	9.600486103029388
>50	0.07756139795067121	3.624832676990556
>100	0.045498614572007136	5.274231794498735
>500	3.053598293423295E-4	0.16343514278403898
>1k	6.10719658684659E-4	0.7373391096446158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2124	0.4418730925499448	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1404	0.2920856035499635	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.162685856108313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.24114537499922E-4	0.0	0.0
2	2.0803817916664066E-4	0.0	8.321527166665626E-4	0.0	0.0
3	2.0803817916664066E-4	0.0	0.0014562672541664845	0.0	0.0
4	2.0803817916664066E-4	0.0	0.0014562672541664845	0.0	0.0
5	2.0803817916664066E-4	0.0	0.0020803817916664065	2.0803817916664066E-4	0.0
6	2.0803817916664066E-4	0.0	0.004576839941666094	2.0803817916664066E-4	0.0
7	2.0803817916664066E-4	0.0	0.009153679883332188	2.0803817916664066E-4	0.0
8	2.0803817916664066E-4	0.0	0.011234061674998595	2.0803817916664066E-4	0.0
9	4.160763583332813E-4	0.0	0.013938558004164924	6.24114537499922E-4	0.0
10	0.001248229074999844	0.0	0.01560286343749805	6.24114537499922E-4	0.0
11	0.0014562672541664845	0.0	0.02184400881249727	8.321527166665626E-4	0.0
12	0.0014562672541664845	0.0	0.024132428783330318	8.321527166665626E-4	0.0
13	0.0014562672541664845	0.0	0.025588696037496802	8.321527166665626E-4	0.0
14	0.0014562672541664845	0.0	0.027044963291663286	8.321527166665626E-4	0.0
15	0.0014562672541664845	0.0	0.029957497799996256	0.0010401908958332033	0.0
16	0.0014562672541664845	0.0	0.03266199412916258	0.0010401908958332033	0.0
17	0.0014562672541664845	0.0	0.033702185024995786	0.0010401908958332033	0.0
18	0.0014562672541664845	0.0	0.03432629956249571	0.001248229074999844	0.0
19	0.0014562672541664845	0.0	0.03495041409999563	0.001248229074999844	0.0
20	0.0014562672541664845	0.0	0.03557452863749555	0.0014562672541664845	0.0
21	0.0014562672541664845	0.0	0.03661471953332875	0.002288419970833047	0.0
22	0.0014562672541664845	0.0	0.03723883407082868	0.002496458149999688	0.0
23	0.0014562672541664845	0.0	0.0378629486083286	0.002496458149999688	0.0
24	0.0014562672541664845	0.0	0.038070986787495244	0.0027044963291663286	0.0
25	0.0014562672541664845	0.0	0.038279024966661884	0.002912534508332969	0.0
26	0.0014562672541664845	0.0	0.038279024966661884	0.0033286108666662506	0.0
27	0.0014562672541664845	0.0	0.038279024966661884	0.003536649045832891	0.0
28	0.0014562672541664845	0.0	0.038695101324995165	0.01248229074999844	0.0
29	0.0014562672541664845	0.0	0.038695101324995165	0.027253001470829926	0.0
30	0.0014562672541664845	0.0	0.038695101324995165	0.05408992658332657	0.0
31	0.0014562672541664845	0.0	0.038695101324995165	0.12232644934998471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCGT	35	8.861856E-4	26.428572	6
GGTATCA	420	0.0	25.107145	1
TCGTTTG	45	1.3216477E-4	24.666666	12
TGTACTA	55	1.8996743E-5	23.545454	5
GTATCAA	1160	0.0	22.646551	1
CGGGGAA	65	6.8914575E-5	19.923077	17
GTTCGTT	50	0.0070290733	18.5	10
TTAAGAC	50	0.0070290733	18.5	3
TTAACGG	70	1.21732504E-4	18.5	35
TTGTACT	105	4.7829235E-7	17.61905	4
TACCGCA	95	3.5975627E-6	17.526316	7
TAGTACC	85	2.7177823E-5	17.411764	4
AACGGCC	75	2.0641153E-4	17.266666	37
TTTAGAC	65	0.0015781266	17.076923	3
CTCTATA	65	0.0015781266	17.076923	2
ACCGACC	110	7.7843833E-7	16.818182	8
GTGTATA	100	5.8634887E-6	16.650002	1
ATAGCCT	80	3.3774454E-4	16.1875	4
GCCGGCA	80	3.3774454E-4	16.1875	15
TTCGCCG	80	3.3774454E-4	16.1875	24
>>END_MODULE