FastQCFastQC Report
Fri 10 Feb 2017
ERR1633003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311223
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81982.6341240846595526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63232.0316621843501284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34391.104995453420859No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG17270.5549075743116672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.49546466681447066No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13490.4334512552092873No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12600.4048543970079332No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12230.39296581550849385No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11290.36276239223964807No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10200.32773927376832684No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10120.32516876966034003No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.30267685871545486No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8040.25833566285268117No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7070.22716830054334028No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6980.22427648342185505No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6800.2184928491788846No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6570.2111026498684223No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6560.21078133685492398No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6500.2088534587739338No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6460.20756820671994036No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6350.2040337635714584No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6210.19953538138248136No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6140.19728619028799285No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5990.19246649508551744No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5870.18861073892353716No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5860.18828942591003878No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5830.1873254868695437No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5830.1873254868695437No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5820.18700417385604534No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5750.18475498276155683No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.18379104372106175No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5720.18379104372106175No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5450.17511559235660604No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5380.17286640126211752No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5350.17190246222162242No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5290.16997458414063227No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5210.1674040800326454No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5150.16547620195165524No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5080.16322701085716673No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5000.16065650674917986No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4970.15969256770868476No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4940.1587286286681897No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4860.1561581245602028No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4790.1539089334657143No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4760.1529449944252192No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4710.15133842935772743No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4690.1506958033307307No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4570.14684004716875038No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4510.14491216908776022No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.14330560402026843No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4450.14298429100677007No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.14137772593927825No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4140.13302358758832092No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4130.13270227457482256No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4100.13173833553432748No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4060.13045308348033402No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3970.1275612663588488No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3830.12306288416987175No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3820.12274157115637341No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3810.12242025814287504No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3760.12081369307538324No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3740.12017106704838652No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3710.11920712800789146No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3710.11920712800789146No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3700.11888581499439309No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3670.117921875953898No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG3660.11760056294039965No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC3600.1156726848594095No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG3550.11406611979191769No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3470.11149561568393081No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3470.11149561568393081No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3470.11149561568393081No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3450.11085298965693409No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3440.11053167664343574No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3390.10892511157594394No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3380.10860379856244558No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3360.10796117253544886No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.10571198144096033No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3280.10539066842746199No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3260.10474804240046526No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3230.10378410335997018No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3230.10378410335997018No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3200.1028201643194751No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3160.10153491226548167No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3160.10153491226548167No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3150.1012135992519833No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3140.10089228623848495No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3130.10057097322498658No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG3130.10057097322498658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC507.2959665E-933.3000039
GAGGATC250.00549026229.636
TCGTTGC250.00549026229.616
GGGCGTC250.00549026229.67
ATGCGGT250.00549026229.64
GCTCGTT250.00549026229.614
GGTATCA14150.027.7173141
TAGTGCA556.2371146E-726.9090910
ACTAGTG1200.024.6666688
GAATCAA751.36551535E-824.6666661
GTATCAA36500.022.5547941
TGACATC502.695971E-422.217
GGCAGCT4200.022.02381118
TTCGCCG3950.022.01265724
GCCGCTC4100.021.20731527
GGACCGT1059.749783E-1021.1428596
GACCGTT705.08222E-621.1428577
CGGCAGC4550.021.14285717
CAGCTTC4300.021.08139420
CCGGCAG4400.021.02272816
Produced by FastQC (version 0.11.5)