FastQCFastQC Report
Fri 10 Feb 2017
ERR1633002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339818
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6980.20540406923706217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.14066353165518014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC250.00549096329.630
CGCCCCT250.00549096329.634
GGTATCA1600.024.2812521
GTATCAA4350.022.5402281
ACTAATA555.132854E-420.18181821
AACTGTA759.234311E-619.7333347
CGTAGGT500.00702493618.527
ATGCGGA609.218573E-418.56
CTACACT701.2159933E-418.54
TTAGGAC752.0618721E-417.2666663
ATAATAC752.0618721E-417.2666663
TGTTATA650.001576839717.0769232
TAGGACC650.001576839717.0769234
CGAACTA904.4311477E-516.44444524
TCTTAAA803.3738016E-416.18752
GGCTTAT803.3738016E-416.18751
TGCTCGG803.3738016E-416.187510
TATACAC803.3738016E-416.18753
TAATACT1059.304345E-615.8571424
TTATGCG700.002587458615.8571424
Produced by FastQC (version 0.11.5)