##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633002.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339818
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266783984368104	33.0	33.0	33.0	27.0	33.0
2	30.919280320642226	33.0	33.0	33.0	27.0	33.0
3	31.347927419971867	33.0	33.0	33.0	27.0	33.0
4	31.51525228210395	33.0	33.0	33.0	27.0	33.0
5	31.623939873697097	33.0	33.0	33.0	27.0	33.0
6	34.411570311166564	37.0	33.0	37.0	27.0	37.0
7	34.60847865622186	37.0	37.0	37.0	27.0	37.0
8	34.75882384099724	37.0	37.0	37.0	27.0	37.0
9	34.881095174475746	37.0	37.0	37.0	27.0	37.0
10	34.88896409254365	37.0	37.0	37.0	27.0	37.0
11	34.895894272816626	37.0	37.0	37.0	27.0	37.0
12	34.866157766804584	37.0	37.0	37.0	27.0	37.0
13	34.89771877887575	37.0	37.0	37.0	27.0	37.0
14	34.87597772925507	37.0	37.0	37.0	27.0	37.0
15	34.911423173581156	37.0	37.0	37.0	27.0	37.0
16	34.89325462453431	37.0	37.0	37.0	27.0	37.0
17	34.86917408730556	37.0	37.0	37.0	27.0	37.0
18	34.86412432537417	37.0	37.0	37.0	27.0	37.0
19	34.867458463059634	37.0	37.0	37.0	27.0	37.0
20	34.876018927778986	37.0	37.0	37.0	27.0	37.0
21	34.880338887286726	37.0	37.0	37.0	27.0	37.0
22	34.88516205733657	37.0	37.0	37.0	27.0	37.0
23	34.85952186170244	37.0	37.0	37.0	27.0	37.0
24	34.848980925083424	37.0	37.0	37.0	27.0	37.0
25	34.85273881901488	37.0	37.0	37.0	27.0	37.0
26	34.794395823646774	37.0	37.0	37.0	27.0	37.0
27	34.755483817808354	37.0	37.0	37.0	27.0	37.0
28	34.71094527070373	37.0	37.0	37.0	27.0	37.0
29	34.74656139462889	37.0	37.0	37.0	27.0	37.0
30	34.6940273911329	37.0	37.0	37.0	27.0	37.0
31	34.69910952333308	37.0	37.0	37.0	27.0	37.0
32	34.725782624816816	37.0	37.0	37.0	27.0	37.0
33	34.72339899593312	37.0	37.0	37.0	27.0	37.0
34	34.637797291491324	37.0	37.0	37.0	27.0	37.0
35	34.627794878434926	37.0	37.0	37.0	27.0	37.0
36	34.59843504464154	37.0	37.0	37.0	27.0	37.0
37	34.650592375918876	37.0	37.0	37.0	27.0	37.0
38	34.49521214297065	37.0	37.0	37.0	27.0	37.0
39	34.58483070349423	37.0	37.0	37.0	27.0	37.0
40	34.61918438693654	37.0	37.0	37.0	27.0	37.0
41	34.61744522067695	37.0	37.0	37.0	27.0	37.0
42	34.58074322137144	37.0	37.0	37.0	27.0	37.0
43	34.340246838013286	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	15.0
17	27.0
18	11.0
19	19.0
20	44.0
21	117.0
22	347.0
23	751.0
24	1407.0
25	2485.0
26	3836.0
27	5681.0
28	7671.0
29	10019.0
30	12571.0
31	15774.0
32	19894.0
33	25097.0
34	34800.0
35	60203.0
36	139036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80890358956854	20.26437681347074	13.375395064416834	25.55132453254389
2	16.206027932599216	22.52235019922429	37.33704512415469	23.9345767440218
3	18.140887180785008	26.490650877822837	29.542578674466917	25.82588326692524
4	13.280344184239798	17.87839372840756	38.13659076329094	30.704671324061707
5	14.744951709444468	36.51719449823141	34.88543867599715	13.852415116326974
6	30.649641867117104	38.97939485253871	17.20921198994756	13.161751290396623
7	27.318152658187618	31.510691016956134	23.257743851120306	17.91341247373594
8	24.778263658782056	36.2023200654468	20.186688168372484	18.832728107398665
9	25.921522697443926	15.631897074316251	19.839443466796933	38.60713676144289
10	15.124566679810957	28.196269767934602	33.58327104508884	23.0958925071656
11	34.76037172839579	23.253035448387077	23.74624063469269	18.240352188524444
12	23.255978200095345	25.328558228228054	29.975163175582225	21.44030039609438
13	28.336344749248127	21.730749989700367	25.744663319777057	24.18824194127445
14	22.1383211012954	20.524516064481578	27.106274535192366	30.230888299030656
15	23.98754627477061	28.59677827542979	23.507583471152206	23.908091978647395
16	23.665609237886162	27.989394322843403	24.56314850890771	23.781847930362723
17	23.000253076646914	27.36141110829915	26.035407188553872	23.602928626500066
18	23.557904525363575	27.146001683253978	26.822887545686218	22.473206245696225
19	24.916867264241446	26.289366660977347	26.19696425733775	22.596801817443453
20	24.457797997751737	26.22668604959125	26.553625764379756	22.761890188277253
21	23.663255036519548	26.744610350246305	26.54950591198818	23.042628701245963
22	23.36574283881372	26.425027514728473	26.683989665056	23.52523998140181
23	23.170344125384766	26.465048937960912	26.984738889640923	23.379868047013403
24	23.40488143653367	26.866440270968578	25.75967135348922	23.969006939008526
25	23.80215291714977	26.387654568033476	26.757264182591854	23.052928332224898
26	23.61528818367479	26.78257184728296	26.410019481016313	23.192120488025942
27	23.477861678898705	26.74078477302556	26.079548464177883	23.70180508389785
28	23.432543302591387	26.531260851396926	26.75696990742103	23.27922593859066
29	24.182062162687085	26.565102496041998	26.133400820439178	23.11943452083174
30	23.795384588220752	26.309083097422736	26.438269897415672	23.457262416940832
31	23.731232600980526	26.27906702999841	26.57746205321672	23.41223831580434
32	22.87430330353307	26.346456044117733	26.668981631343836	24.110259021005362
33	23.083238674820052	26.4803512468439	26.984150339299273	23.45225973903678
34	23.65972373446963	26.050120947095213	26.783748947966263	23.5064063704689
35	23.52965410896421	26.72930804136332	26.3911858700834	23.349851979589072
36	24.18677056542031	26.468285964840003	26.13222371975587	23.212719749983815
37	23.527594182768425	26.12751531702264	26.395894272816626	23.94899622739231
38	23.657075257932185	26.485942475089608	26.326739607672344	23.530242659305863
39	23.082650124478395	26.46681458898587	26.699291973938994	23.751243312596742
40	23.245090018774757	26.648382369385963	27.025348863215015	23.081178748624264
41	23.344260751343366	26.030110235478993	26.848783760718973	23.77684525245867
42	23.179172380509566	26.55038873750066	26.725188188971742	23.545250693018026
43	23.78037655450859	25.88061844869901	26.67339575890624	23.665609237886162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.0
2	4.0
3	32.0
4	60.0
5	60.0
6	80.0
7	100.0
8	91.5
9	83.0
10	128.0
11	173.0
12	173.0
13	256.0
14	339.0
15	653.5
16	968.0
17	1145.0
18	1322.0
19	1322.0
20	1391.0
21	1460.0
22	1697.0
23	1934.0
24	2655.0
25	3376.0
26	3376.0
27	4714.5
28	6053.0
29	7602.5
30	9152.0
31	10994.0
32	12836.0
33	12836.0
34	14942.5
35	17049.0
36	18841.0
37	20633.0
38	22288.0
39	23943.0
40	23943.0
41	25242.0
42	26541.0
43	26949.0
44	27357.0
45	27153.0
46	26949.0
47	26949.0
48	26197.5
49	25446.0
50	25185.0
51	24924.0
52	23724.0
53	22524.0
54	22524.0
55	21808.0
56	21092.0
57	19017.0
58	16942.0
59	15690.5
60	14439.0
61	14439.0
62	12700.0
63	10961.0
64	9559.0
65	8157.0
66	6811.5
67	5466.0
68	5466.0
69	4546.5
70	3627.0
71	3033.0
72	2439.0
73	1959.5
74	1480.0
75	1480.0
76	1145.0
77	810.0
78	626.5
79	443.0
80	373.0
81	303.0
82	303.0
83	242.5
84	182.0
85	162.0
86	142.0
87	100.0
88	58.0
89	58.0
90	46.0
91	34.0
92	21.0
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	339818.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.39808045877099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.28298346752219	64.63916620680791
2	6.15491831502097	8.912085427761028
3	1.7339768942040898	3.766097961007126
4	0.8072558998692602	2.3377511035820913
5	0.47830366311764755	1.7314133543058174
6	0.30870585436297243	1.3409826769358473
7	0.22061652910000526	1.1180549257021881
8	0.14533749247895156	0.8417724379333729
9	0.11371395563662624	0.7409404898519024
>10	0.68184558779742	9.328370781026125
>50	0.04986308242135147	2.433489320488283
>100	0.02207054467818058	2.603336459334007
>500	4.087137903366774E-4	0.20653885526428015
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.20540406923706217	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.14066353165518014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	8.8282551248021E-4	0.0	0.0
7	0.0	0.0	0.00264847653744063	0.0	0.0
8	0.0	0.0	0.0029427517082673666	0.0	0.0
9	0.0	0.0	0.00441412756240105	2.942751708267367E-4	0.0
10	2.942751708267367E-4	0.0	0.00441412756240105	2.942751708267367E-4	0.0
11	2.942751708267367E-4	0.0	0.00529695307488126	2.942751708267367E-4	0.0
12	2.942751708267367E-4	0.0	0.005591228245707997	2.942751708267367E-4	0.0
13	2.942751708267367E-4	0.0	0.005885503416534733	5.885503416534734E-4	0.0
14	2.942751708267367E-4	0.0	0.006474053758188206	5.885503416534734E-4	0.0
15	2.942751708267367E-4	0.0	0.006768328929014944	5.885503416534734E-4	0.0
16	2.942751708267367E-4	0.0	0.006768328929014944	5.885503416534734E-4	0.0
17	2.942751708267367E-4	0.0	0.006768328929014944	5.885503416534734E-4	0.0
18	2.942751708267367E-4	0.0	0.006768328929014944	5.885503416534734E-4	0.0
19	2.942751708267367E-4	0.0	0.006768328929014944	5.885503416534734E-4	0.0
20	2.942751708267367E-4	0.0	0.00706260409984168	5.885503416534734E-4	0.0
21	2.942751708267367E-4	0.0	0.00794542961232189	0.0014713758541336833	0.0
22	2.942751708267367E-4	0.0	0.008239704783148626	0.00176565102496042	0.0
23	2.942751708267367E-4	0.0	0.008239704783148626	0.00176565102496042	0.0
24	2.942751708267367E-4	0.0	0.008239704783148626	0.0020599261957871566	0.0
25	2.942751708267367E-4	0.0	0.008239704783148626	0.0020599261957871566	0.0
26	2.942751708267367E-4	0.0	0.008239704783148626	0.00264847653744063	0.0
27	2.942751708267367E-4	0.0	0.008239704783148626	0.00264847653744063	0.0
28	2.942751708267367E-4	0.0	0.008239704783148626	0.0088282551248021	0.0
29	2.942751708267367E-4	0.0	0.008533979953975363	0.01589085922464378	0.0
30	2.942751708267367E-4	0.0	0.008533979953975363	0.02825041639936672	0.0
31	2.942751708267367E-4	0.0	0.008533979953975363	0.08239704783148627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGC	25	0.005490963	29.6	30
CGCCCCT	25	0.005490963	29.6	34
GGTATCA	160	0.0	24.281252	1
GTATCAA	435	0.0	22.540228	1
ACTAATA	55	5.132854E-4	20.181818	21
AACTGTA	75	9.234311E-6	19.733334	7
CGTAGGT	50	0.007024936	18.5	27
ATGCGGA	60	9.218573E-4	18.5	6
CTACACT	70	1.2159933E-4	18.5	4
TTAGGAC	75	2.0618721E-4	17.266666	3
ATAATAC	75	2.0618721E-4	17.266666	3
TGTTATA	65	0.0015768397	17.076923	2
TAGGACC	65	0.0015768397	17.076923	4
CGAACTA	90	4.4311477E-5	16.444445	24
TCTTAAA	80	3.3738016E-4	16.1875	2
GGCTTAT	80	3.3738016E-4	16.1875	1
TGCTCGG	80	3.3738016E-4	16.1875	10
TATACAC	80	3.3738016E-4	16.1875	3
TAATACT	105	9.304345E-6	15.857142	4
TTATGCG	70	0.0025874586	15.857142	4
>>END_MODULE