FastQCFastQC Report
Fri 10 Feb 2017
ERR1633001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484321
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13480.27832780325445317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.1901631356063437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4980.10282436648421192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG200.001840884337.013
TTGCCGC358.8619575E-426.4285710
GGTATCA2850.025.3157881
AACGGCC1300.022.76922837
GTATCAA8100.022.611111
TTAACGG1250.022.19999935
TAACGGC1301.8189894E-1221.34615136
TTCGTCC450.003822659120.55555716
GAGACGC555.137191E-420.18181824
TAGGACC851.2422661E-619.5882364
CTAGACC500.00702914818.54
ATCTCGT500.00702914818.537
CTCGTGA500.00702914818.51
GTGGCGG500.00702914818.533
CGAGACG500.00702914818.523
GCACCGC1552.0008883E-1117.90322710
TCGGGAT1156.382288E-817.69565222
GTTCTAT852.71785E-517.4117641
CACCGCC1603.45608E-1117.3437511
GCCCACG752.0641566E-417.26666618
Produced by FastQC (version 0.11.5)