FastQCFastQC Report
Fri 10 Feb 2017
ERR1633000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480185
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13350.2780178472880244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.19013505211533055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5000.10412653456480314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7450.023.8389261
GGTATCA2750.023.5454541
CGCCGTC450.003822612820.55555526
GCTTCGC752.0641088E-417.26666622
CGAACTA650.001578123317.07692329
GTCTTAT650.001578123317.0769231
GTATTAA1553.9835868E-1016.7096791
ATACTAG904.437288E-516.4444456
TCATATA803.3774378E-416.18752
GTTCTAG700.002589546815.8571431
GCGAACT700.002589546815.85714328
GCGAACC700.002589546815.85714330
AGCTTCG700.002589546815.85714321
ATTATAC700.002589546815.8571433
AAGTGCG700.002589546815.85714316
AGGTATT855.3560775E-415.2352947
ATCAACG11950.015.1715473
TCAACGC12050.015.0456434
CGAACCG750.00409977914.831
GCACCCT750.00409977914.86
Produced by FastQC (version 0.11.5)