##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633000.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480185
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734671012214044	33.0	33.0	33.0	27.0	33.0
2	31.232918562637316	33.0	33.0	33.0	27.0	33.0
3	31.692193633703678	33.0	33.0	33.0	27.0	33.0
4	31.828703520518133	33.0	33.0	33.0	27.0	33.0
5	31.939816945552234	33.0	33.0	33.0	33.0	33.0
6	34.84394972770911	37.0	37.0	37.0	27.0	37.0
7	35.02145214865104	37.0	37.0	37.0	33.0	37.0
8	35.19872757374762	37.0	37.0	37.0	33.0	37.0
9	35.29315576288305	37.0	37.0	37.0	33.0	37.0
10	35.319314430896426	37.0	37.0	37.0	33.0	37.0
11	35.353126399200306	37.0	37.0	37.0	33.0	37.0
12	35.29668981746619	37.0	37.0	37.0	33.0	37.0
13	35.34627279069525	37.0	37.0	37.0	33.0	37.0
14	35.302735404063014	37.0	37.0	37.0	33.0	37.0
15	35.34460260108084	37.0	37.0	37.0	33.0	37.0
16	35.33312160937972	37.0	37.0	37.0	33.0	37.0
17	35.29092745504337	37.0	37.0	37.0	33.0	37.0
18	35.30137967658298	37.0	37.0	37.0	33.0	37.0
19	35.321807220133906	37.0	37.0	37.0	33.0	37.0
20	35.32621593760738	37.0	37.0	37.0	33.0	37.0
21	35.32242781427991	37.0	37.0	37.0	33.0	37.0
22	35.326755313056424	37.0	37.0	37.0	33.0	37.0
23	35.31840853004571	37.0	37.0	37.0	33.0	37.0
24	35.31387277820007	37.0	37.0	37.0	33.0	37.0
25	35.30394118933328	37.0	37.0	37.0	33.0	37.0
26	35.27789081291586	37.0	37.0	37.0	33.0	37.0
27	35.23471786915459	37.0	37.0	37.0	33.0	37.0
28	35.194670803960975	37.0	37.0	37.0	33.0	37.0
29	35.218965607005636	37.0	37.0	37.0	33.0	37.0
30	35.18180492935014	37.0	37.0	37.0	33.0	37.0
31	35.183200224913314	37.0	37.0	37.0	33.0	37.0
32	35.212678446848614	37.0	37.0	37.0	33.0	37.0
33	35.191765673646614	37.0	37.0	37.0	33.0	37.0
34	35.12145943750846	37.0	37.0	37.0	33.0	37.0
35	35.13582056915564	37.0	37.0	37.0	33.0	37.0
36	35.10543436383894	37.0	37.0	37.0	33.0	37.0
37	35.13047887793246	37.0	37.0	37.0	33.0	37.0
38	34.988050438893346	37.0	37.0	37.0	27.0	37.0
39	35.07161614794298	37.0	37.0	37.0	27.0	37.0
40	35.10700459198017	37.0	37.0	37.0	33.0	37.0
41	35.13335277028645	37.0	37.0	37.0	33.0	37.0
42	35.04242531524309	37.0	37.0	37.0	27.0	37.0
43	34.82402615658548	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	25.0
17	24.0
18	15.0
19	28.0
20	29.0
21	66.0
22	242.0
23	558.0
24	1092.0
25	2072.0
26	3538.0
27	5518.0
28	7821.0
29	10929.0
30	14081.0
31	18375.0
32	23518.0
33	30861.0
34	44236.0
35	81369.0
36	235776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57730874558764	19.605152180930265	13.238855857638201	25.578683215843895
2	16.060684944344366	22.516530087362163	37.79647427553964	23.626310692753837
3	18.30357049887023	25.964576152941056	29.67627060403803	26.05558274415069
4	13.098285035975717	17.432656163770215	38.21339691993711	31.25566188031696
5	14.701833668273686	36.206878598873345	34.814498578672804	14.276789154180157
6	30.826035798702584	38.515780376313295	17.166508741422575	13.491675083561544
7	27.22513198038256	31.648635421764524	23.0956818726116	18.030550725241312
8	24.984745462686256	35.72935431135916	20.255943021960285	19.029957203994293
9	26.21093953372138	15.406978560346532	19.636598394368836	38.74548351156325
10	15.104386850901216	27.779293397336442	33.753449191457456	23.362870560304884
11	34.62373876735008	22.99946895467372	23.96638795464248	18.410404323333715
12	23.287066443141704	24.864583441798473	30.352676572570985	21.495673542488834
13	28.632922727698702	21.50212938763185	25.783395982798297	24.081551901871155
14	22.477170257296667	20.31342086904006	27.1895207055614	30.019888168101875
15	24.18838572633464	28.101252642210817	23.712943969511752	23.997417661942794
16	23.567166821121027	27.829065880858415	24.732759249039432	23.87100804898112
17	22.969897018857317	27.05769651280236	26.196153565813173	23.77625290252715
18	23.850599248206418	26.693045388756413	26.846736153774064	22.609619209263094
19	24.975165821506295	26.073076001957578	26.550600289471767	22.40115788706436
20	24.595728729552153	26.074533773441487	26.472088882409906	22.857648614596457
21	23.732103251871674	26.386913377135894	26.635775794745776	23.245207576246653
22	23.489696679404812	26.186573924633215	26.66513947749305	23.658589918468923
23	23.37474098524527	26.11493486885263	26.966273415454463	23.54405073044764
24	23.783541759946687	26.360256984287307	26.17282922207066	23.683372033695345
25	23.825608879910867	26.060789070878933	26.82861813675979	23.28498391245041
26	23.668794318856275	26.41107073315493	26.531024500973583	23.389110447015213
27	23.70357258140092	26.377333735955933	26.34942782469257	23.569665857950582
28	23.556337661526285	26.154086445848996	26.737819798619284	23.551756094005434
29	24.058852317336026	26.206566219269657	26.314857815217053	23.419723648177264
30	23.837479304851257	26.33714089361392	26.49603798535981	23.329341816175017
31	23.84039484781907	26.0457948499016	26.393160969209784	23.720649333069545
32	23.39452502681258	26.037048220998155	26.715744973291542	23.852681778897715
33	23.257286254256172	26.19261326363797	26.947322386163663	23.60277809594219
34	23.637556358486833	25.763820194300113	26.863396399304435	23.73522704790862
35	23.52780699105553	26.29465726751148	26.67805116777909	23.499484573653902
36	24.014077907473162	26.278413528119373	26.364630298739026	23.34287826566844
37	23.707112883576123	25.911055114174747	26.43293730541354	23.948894696835595
38	23.68649582973229	26.310067994627072	26.426481460270523	23.576954715370118
39	23.38536189177088	26.077865822547558	26.622655851390608	23.91411643429095
40	23.39119297770651	26.245301290127763	26.77905390630694	23.584451825858785
41	23.5684163395358	25.81213490633818	26.78301071462041	23.83643803950561
42	23.366410862480087	26.3848308464446	26.7069983443881	23.541759946687215
43	23.69253516873705	25.615127502941576	26.852567239709696	23.83977008861168
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	66.5
4	117.0
5	117.0
6	168.5
7	220.0
8	205.0
9	190.0
10	266.0
11	342.0
12	342.0
13	518.5
14	695.0
15	1196.0
16	1697.0
17	2009.5
18	2322.0
19	2322.0
20	2200.0
21	2078.0
22	2523.5
23	2969.0
24	3993.5
25	5018.0
26	5018.0
27	6785.5
28	8553.0
29	10669.5
30	12786.0
31	15304.0
32	17822.0
33	17822.0
34	20647.0
35	23472.0
36	26015.0
37	28558.0
38	30513.0
39	32468.0
40	32468.0
41	33987.0
42	35506.0
43	35968.0
44	36430.0
45	36799.5
46	37169.0
47	37169.0
48	36648.5
49	36128.0
50	35702.5
51	35277.0
52	33914.0
53	32551.0
54	32551.0
55	31067.5
56	29584.0
57	26966.5
58	24349.0
59	22763.5
60	21178.0
61	21178.0
62	18792.5
63	16407.0
64	14201.0
65	11995.0
66	10413.0
67	8831.0
68	8831.0
69	7361.5
70	5892.0
71	4937.0
72	3982.0
73	3229.5
74	2477.0
75	2477.0
76	1906.0
77	1335.0
78	1038.0
79	741.0
80	604.5
81	468.0
82	468.0
83	361.5
84	255.0
85	212.5
86	170.0
87	128.0
88	86.0
89	86.0
90	59.0
91	32.0
92	20.0
93	8.0
94	6.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	480185.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.72993211289837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.05003098607543	69.99582795312038
2	6.3688927574560035	9.901072033427704
3	1.5578721406860052	3.632798872082965
4	0.6282174496063864	1.9532519884017028
5	0.34091208694027153	1.3249536687166905
6	0.22848952642512999	1.0656285226520192
7	0.14536342116298642	0.7909362201088314
8	0.09823228157467695	0.6108470862475806
9	0.07542464800427419	0.527647749211034
>10	0.46193221399754936	6.61030185300955
>50	0.032802191125537096	1.7676610018043943
>100	0.011292556175439358	1.3492573091288758
>500	2.6887038512948537E-4	0.19081039703138053
>1k	2.6887038512948537E-4	0.27900534505683794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1335	0.2780178472880244	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	913	0.19013505211533055	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.10412653456480314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	2.082530691296063E-4	0.0	0.0
3	0.0	0.0	2.082530691296063E-4	0.0	0.0
4	0.0	0.0	2.082530691296063E-4	2.082530691296063E-4	0.0
5	2.082530691296063E-4	0.0	4.165061382592126E-4	2.082530691296063E-4	0.0
6	2.082530691296063E-4	0.0	0.0012495184147776378	2.082530691296063E-4	0.0
7	2.082530691296063E-4	0.0	0.00562283286649937	4.165061382592126E-4	0.0
8	2.082530691296063E-4	0.0	0.005831085935628976	4.165061382592126E-4	0.0
9	2.082530691296063E-4	0.0	0.007705363557795433	0.0010412653456480314	0.0
10	0.0010412653456480314	0.0	0.008121869696054645	0.0010412653456480314	0.0
11	0.0010412653456480314	0.0	0.012911690286035591	0.0010412653456480314	0.0
12	0.0010412653456480314	0.0	0.016451992461238896	0.0010412653456480314	0.0
13	0.0010412653456480314	0.0	0.018742776221664568	0.0010412653456480314	0.0
14	0.0010412653456480314	0.0	0.020200547705571812	0.0010412653456480314	0.0
15	0.0010412653456480314	0.0	0.02249133146599748	0.0010412653456480314	0.0
16	0.0010412653456480314	0.0	0.024782115226423148	0.0010412653456480314	0.0
17	0.0010412653456480314	0.0	0.02540687443381197	0.0010412653456480314	0.0
18	0.0010412653456480314	0.0	0.025823380572071182	0.0010412653456480314	0.0
19	0.0010412653456480314	0.0	0.026239886710330392	0.0010412653456480314	0.0
20	0.0010412653456480314	0.0	0.02644813977946	0.0010412653456480314	0.0
21	0.0010412653456480314	0.0	0.02748940512510803	0.001457771483907244	0.0
22	0.0010412653456480314	0.0	0.027905911263367243	0.002082530691296063	0.0
23	0.0010412653456480314	0.0	0.028322417401626457	0.0024990368295552757	0.0
24	0.0010412653456480314	0.0	0.028530670470756064	0.0024990368295552757	0.0
25	0.0010412653456480314	0.0	0.028530670470756064	0.0024990368295552757	0.0
26	0.0010412653456480314	0.0	0.028530670470756064	0.0031237960369440943	0.0
27	0.0010412653456480314	0.0	0.028530670470756064	0.0037485552443329135	0.0
28	0.0010412653456480314	0.0	0.028530670470756064	0.011037412663869133	0.0
29	0.0010412653456480314	0.0	0.028530670470756064	0.02707289898684882	0.0
30	0.0010412653456480314	0.0	0.028530670470756064	0.04435790372460614	0.0
31	0.0010412653456480314	0.0	0.028530670470756064	0.10454304070306236	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	745	0.0	23.838926	1
GGTATCA	275	0.0	23.545454	1
CGCCGTC	45	0.0038226128	20.555555	26
GCTTCGC	75	2.0641088E-4	17.266666	22
CGAACTA	65	0.0015781233	17.076923	29
GTCTTAT	65	0.0015781233	17.076923	1
GTATTAA	155	3.9835868E-10	16.709679	1
ATACTAG	90	4.437288E-5	16.444445	6
TCATATA	80	3.3774378E-4	16.1875	2
GTTCTAG	70	0.0025895468	15.857143	1
GCGAACT	70	0.0025895468	15.857143	28
GCGAACC	70	0.0025895468	15.857143	30
AGCTTCG	70	0.0025895468	15.857143	21
ATTATAC	70	0.0025895468	15.857143	3
AAGTGCG	70	0.0025895468	15.857143	16
AGGTATT	85	5.3560775E-4	15.235294	7
ATCAACG	1195	0.0	15.171547	3
TCAACGC	1205	0.0	15.045643	4
CGAACCG	75	0.004099779	14.8	31
GCACCCT	75	0.004099779	14.8	6
>>END_MODULE