FastQCFastQC Report
Fri 10 Feb 2017
ERR1632999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377386
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11910.31559199334368526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.22364369637453432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4540.12030123004033005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA401.5962996E-632.37524
CGAGCCC401.5962996E-632.37515
GCGAATC453.9988827E-628.77777733
GGTATCA2300.026.5434761
GTATGCT358.8583125E-426.4285723
GGCGAAT509.069974E-625.90000232
TCTCCGA601.333432E-624.66666611
AGACTAA551.8982706E-523.54545425
ATCTCGT551.8982706E-523.54545437
ATCTCCG551.8982706E-523.54545410
TCACGGT802.7135684E-823.1251
CCGTCCG400.001928797323.12528
CGCCGTC400.001928797323.12526
CACGGTA652.674924E-622.769232
GTATCAA7750.022.438711
ACTCTAT502.6974437E-422.21
CGACTCG502.6974437E-422.234
ACTAAGG603.7177553E-521.58333227
GCCCACG705.086951E-621.14285918
TACCGCA705.086951E-621.1428597
Produced by FastQC (version 0.11.5)