##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632999.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377386
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.134461797734946	33.0	33.0	33.0	27.0	33.0
2	30.828841027489094	33.0	33.0	33.0	27.0	33.0
3	31.263687577175624	33.0	33.0	33.0	27.0	33.0
4	31.427726518736783	33.0	33.0	33.0	27.0	33.0
5	31.53259262399771	33.0	33.0	33.0	27.0	33.0
6	34.25649335163467	37.0	33.0	37.0	27.0	37.0
7	34.38781777808398	37.0	37.0	37.0	27.0	37.0
8	34.60517613265993	37.0	37.0	37.0	27.0	37.0
9	34.66449470833576	37.0	37.0	37.0	27.0	37.0
10	34.73024966479944	37.0	37.0	37.0	27.0	37.0
11	34.74894670178544	37.0	37.0	37.0	27.0	37.0
12	34.67935747484008	37.0	37.0	37.0	27.0	37.0
13	34.73419787697477	37.0	37.0	37.0	27.0	37.0
14	34.68423841901925	37.0	37.0	37.0	27.0	37.0
15	34.74181077199472	37.0	37.0	37.0	27.0	37.0
16	34.74479445448427	37.0	37.0	37.0	27.0	37.0
17	34.72856173784931	37.0	37.0	37.0	27.0	37.0
18	34.69227793293869	37.0	37.0	37.0	27.0	37.0
19	34.71093257301543	37.0	37.0	37.0	27.0	37.0
20	34.63595098917289	37.0	37.0	37.0	27.0	37.0
21	34.71857726571733	37.0	37.0	37.0	27.0	37.0
22	34.71404344623277	37.0	37.0	37.0	27.0	37.0
23	34.684633240236785	37.0	37.0	37.0	27.0	37.0
24	34.67304298516638	37.0	37.0	37.0	27.0	37.0
25	34.693072874987415	37.0	37.0	37.0	27.0	37.0
26	34.631809341099036	37.0	37.0	37.0	27.0	37.0
27	34.55747431012279	37.0	37.0	37.0	27.0	37.0
28	34.52815154775217	37.0	37.0	37.0	27.0	37.0
29	34.57374677386019	37.0	37.0	37.0	27.0	37.0
30	34.54259564477749	37.0	37.0	37.0	27.0	37.0
31	34.53251047998601	37.0	37.0	37.0	27.0	37.0
32	34.55817120931884	37.0	37.0	37.0	27.0	37.0
33	34.51889047288453	37.0	37.0	37.0	27.0	37.0
34	34.45496918274658	37.0	37.0	37.0	27.0	37.0
35	34.43375747907977	37.0	37.0	37.0	27.0	37.0
36	34.416395944735626	37.0	37.0	37.0	27.0	37.0
37	34.44337362806252	37.0	37.0	37.0	27.0	37.0
38	34.309386145749976	37.0	37.0	37.0	27.0	37.0
39	34.37241710079335	37.0	37.0	37.0	27.0	37.0
40	34.401533178231304	37.0	37.0	37.0	27.0	37.0
41	34.43664841833031	37.0	37.0	37.0	27.0	37.0
42	34.27757256496002	37.0	37.0	37.0	27.0	37.0
43	34.072641804412456	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	10.0
16	21.0
17	28.0
18	17.0
19	30.0
20	63.0
21	165.0
22	428.0
23	980.0
24	1856.0
25	3144.0
26	4853.0
27	6994.0
28	9310.0
29	12080.0
30	15135.0
31	18664.0
32	23163.0
33	29502.0
34	40942.0
35	69226.0
36	140772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95726921507422	20.392383395250484	13.519579422660088	25.130767967015206
2	16.425092610748678	23.23032650919748	37.13253803797703	23.212042842076812
3	18.226696273841636	27.060092319269923	29.996343266575863	24.71686814031257
4	13.114953919859243	18.62761204708177	38.748390242351334	29.509043790707658
5	14.453106368545733	36.833904808339476	35.07681789997509	13.636170923139703
6	29.277715654528784	40.10933102976793	17.399956543168003	13.212996772535282
7	26.615984694715756	32.06822722623521	23.60712904029296	17.708659038756075
8	24.279914994196922	35.91018214772143	20.56409087777501	19.245811980306634
9	26.007059085392676	15.437774586232663	20.118393369123392	38.43677295925127
10	14.985982521874156	27.97719046281526	33.89632895761899	23.140498057691595
11	34.385218317584645	23.67151934623966	24.01201952377672	17.931242812398978
12	23.146592613398482	25.736778788826292	29.96030589370035	21.156322704074874
13	28.25065052757654	21.90197834577859	26.077278966363355	23.770092160281518
14	22.30315909970163	20.962621824868968	27.389993269490652	29.344225805938745
15	24.124106352646894	28.680184214570758	23.861775476567757	23.333933956214594
16	23.08458713359796	28.061984281345893	25.19542325364481	23.658005331411342
17	22.52971758358816	27.31659362032508	26.288733551324107	23.864955244762655
18	23.530019661566673	26.833268854700492	27.18277837545643	22.453933108276406
19	25.020536002925386	26.139284446163874	26.997291897420677	21.842887653490063
20	24.521842357692126	26.139284446163874	26.897129199281373	22.441743996862627
21	23.404418817868176	26.89421441176938	27.048433169221965	22.652933601140475
22	23.036625629991573	26.637713110714227	26.908258387963517	23.41740287133068
23	23.13811323154542	26.594256278717282	27.521953649578947	22.745676840158353
24	23.11294006666914	27.018490352053337	26.314436677566206	23.55413290371132
25	23.60898390507332	26.579417360474423	27.35051114773733	22.461087586714928
26	23.397529320112564	26.882820242404325	26.980068153031645	22.739582284451462
27	23.213632726174264	26.92760197781582	26.626318941349176	23.232446354660745
28	23.092801534768114	26.617309598130294	27.371179641004172	22.918709226097416
29	23.85727080495832	26.579947321840237	26.74926997821859	22.813511894982856
30	23.661715060972057	26.593461336668554	26.968409002983684	22.776414599375705
31	23.763732623891716	26.51290720906446	26.88891479811122	22.834445368932606
32	22.710964370697376	26.494358561260885	27.155220384433974	23.639456683607765
33	22.743556994695087	26.551594388769058	27.558785964503187	23.14606265203267
34	23.308230829972494	25.95141314198195	27.159990036726324	23.580365991319233
35	23.290477124217645	26.915147885719133	27.02405494639441	22.770320043668814
36	24.158818822107868	26.511847286332824	26.266740154642726	23.06259373691658
37	23.527899816103407	26.07197935270519	26.85658715479641	23.54353367639499
38	23.313000482264844	26.77603302719232	26.787427196557374	23.123539293985466
39	22.948917023948955	26.52933601140477	27.21881574833195	23.30293121631433
40	23.11188014393751	26.70395828144128	27.522483610944764	22.66167796367645
41	23.125659139448736	26.045481284414368	27.14144138892275	23.687418187214153
42	22.762635603864478	26.78451240904538	27.35051114773733	23.102340839352813
43	23.36732152226103	25.94743843173833	27.32560296354396	23.35963708245669
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	8.5
2	13.0
3	56.5
4	100.0
5	100.0
6	127.0
7	154.0
8	169.5
9	185.0
10	237.5
11	290.0
12	290.0
13	440.5
14	591.0
15	1069.5
16	1548.0
17	1808.0
18	2068.0
19	2068.0
20	1901.5
21	1735.0
22	1994.0
23	2253.0
24	3030.0
25	3807.0
26	3807.0
27	4958.0
28	6109.0
29	7937.5
30	9766.0
31	11882.0
32	13998.0
33	13998.0
34	16377.0
35	18756.0
36	20717.0
37	22678.0
38	24901.5
39	27125.0
40	27125.0
41	28827.5
42	30530.0
43	31303.5
44	32077.0
45	32011.0
46	31945.0
47	31945.0
48	31152.5
49	30360.0
50	29930.0
51	29500.0
52	27957.5
53	26415.0
54	26415.0
55	24809.5
56	23204.0
57	20230.0
58	17256.0
59	15843.0
60	14430.0
61	14430.0
62	12502.0
63	10574.0
64	8721.0
65	6868.0
66	5861.0
67	4854.0
68	4854.0
69	4074.5
70	3295.0
71	2686.0
72	2077.0
73	1663.5
74	1250.0
75	1250.0
76	926.0
77	602.0
78	478.0
79	354.0
80	297.0
81	240.0
82	240.0
83	192.0
84	144.0
85	131.5
86	119.0
87	91.0
88	63.0
89	63.0
90	53.0
91	43.0
92	24.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	377386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.06812420120913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.2093257581016	53.257099368994545
2	6.5761836529740245	8.031904001795578
3	1.9193300486349048	3.516296573794474
4	1.0168563021317645	2.483900278134593
5	0.6305635937818619	1.9253667930915763
6	0.4286218719128304	1.5705080225596486
7	0.3115173996292477	1.3316648275977618
8	0.2479628438085769	1.2114100594389754
9	0.18511402790779075	1.0174109802893152
>10	1.3077372932300986	15.577043546938619
>50	0.11963270932232914	5.009234823348219
>100	0.046281267110033164	4.525562498355145
>500	4.366157274531431E-4	0.2250382813062948
>1k	4.366157274531431E-4	0.3175599443552099
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1191	0.31559199334368526	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.22364369637453432	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	454	0.12030123004033005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	5.29961365816432E-4	2.64980682908216E-4	0.0
4	0.0	0.0	7.94942048724648E-4	2.64980682908216E-4	0.0
5	0.0	0.0	7.94942048724648E-4	2.64980682908216E-4	0.0
6	0.0	0.0	0.001854864780357512	2.64980682908216E-4	0.0
7	0.0	0.0	0.004239690926531456	2.64980682908216E-4	0.0
8	0.0	0.0	0.006094555706888968	2.64980682908216E-4	0.0
9	0.0	0.0	0.007419459121430048	0.00132490341454108	0.0
10	2.64980682908216E-4	0.0	0.008479381853062911	0.001589884097449296	0.0
11	2.64980682908216E-4	0.0	0.014043976194135447	0.001589884097449296	0.0
12	2.64980682908216E-4	0.0	0.015368879608676528	0.001589884097449296	0.0
13	2.64980682908216E-4	0.0	0.01589884097449296	0.001589884097449296	0.0
14	2.64980682908216E-4	0.0	0.017488725071942254	0.001589884097449296	0.0
15	2.64980682908216E-4	0.0	0.019608570535207984	0.001589884097449296	0.0
16	2.64980682908216E-4	0.0	0.02199339668138193	0.001854864780357512	0.0
17	2.64980682908216E-4	0.0	0.02199339668138193	0.002119845463265728	0.0
18	2.64980682908216E-4	0.0	0.02252335804719836	0.002119845463265728	0.0
19	2.64980682908216E-4	0.0	0.02305331941301479	0.002119845463265728	0.0
20	2.64980682908216E-4	0.0	0.023318300095923006	0.002119845463265728	0.0
21	2.64980682908216E-4	0.0	0.02384826146173944	0.002119845463265728	0.0
22	2.64980682908216E-4	0.0	0.02384826146173944	0.002384826146173944	0.0
23	2.64980682908216E-4	0.0	0.024113242144647655	0.0029147875119903757	0.0
24	2.64980682908216E-4	0.0	0.024113242144647655	0.0029147875119903757	0.0
25	2.64980682908216E-4	0.0	0.024113242144647655	0.003179768194898592	0.0
26	2.64980682908216E-4	0.0	0.024378222827555873	0.003179768194898592	0.0
27	2.64980682908216E-4	0.0	0.024378222827555873	0.004239690926531456	0.0
28	2.64980682908216E-4	0.0	0.024378222827555873	0.010334246633420424	0.0
29	2.64980682908216E-4	0.0	0.024378222827555873	0.02252335804719836	0.0
30	2.64980682908216E-4	0.0	0.024378222827555873	0.04769652292347888	0.0
31	2.64980682908216E-4	0.0	0.024378222827555873	0.11182184818726715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGACTA	40	1.5962996E-6	32.375	24
CGAGCCC	40	1.5962996E-6	32.375	15
GCGAATC	45	3.9988827E-6	28.777777	33
GGTATCA	230	0.0	26.543476	1
GTATGCT	35	8.8583125E-4	26.428572	3
GGCGAAT	50	9.069974E-6	25.900002	32
TCTCCGA	60	1.333432E-6	24.666666	11
AGACTAA	55	1.8982706E-5	23.545454	25
ATCTCGT	55	1.8982706E-5	23.545454	37
ATCTCCG	55	1.8982706E-5	23.545454	10
TCACGGT	80	2.7135684E-8	23.125	1
CCGTCCG	40	0.0019287973	23.125	28
CGCCGTC	40	0.0019287973	23.125	26
CACGGTA	65	2.674924E-6	22.76923	2
GTATCAA	775	0.0	22.43871	1
ACTCTAT	50	2.6974437E-4	22.2	1
CGACTCG	50	2.6974437E-4	22.2	34
ACTAAGG	60	3.7177553E-5	21.583332	27
GCCCACG	70	5.086951E-6	21.142859	18
TACCGCA	70	5.086951E-6	21.142859	7
>>END_MODULE