FastQCFastQC Report
Fri 10 Feb 2017
ERR1632998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350245
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7540.2152778769147311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.1561763908121458No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3780.10792445288298191No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3730.10649688075490013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG200.001840188837.019
GGTATCA1600.030.0625021
AGCGAGG250.005491189729.629
GCATAGG358.8570354E-426.428571
CTATCCT451.3206471E-424.6666664
AGTACCG652.674009E-622.769235
GTATCAA4550.022.3626371
TAGTACC753.7254176E-722.24
ATAGTAC855.154834E-821.7647063
GCTCTAG450.003820577620.5555551
GTATACC450.003820577620.5555551
CCCTTAA555.1332853E-420.1818181
ACCGCAA801.6114453E-518.58
TAGGCTG500.007025354518.55
TAGTACA500.007025354518.54
TACCGCA801.6114453E-518.57
GCGAACT1054.773756E-717.61904728
TTTAGGT752.0620992E-417.26666619
ACGGGTG752.0620992E-417.2666665
AGAGTGT650.0015769717.0769236
Produced by FastQC (version 0.11.5)