FastQCFastQC Report
Fri 10 Feb 2017
ERR1632997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253788
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6980.2750327044619919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.20450139486500543No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4640.1828297634245906No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4460.17573722949863665No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4420.17416111084842467No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3920.15445962772077482No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3530.13909247088120794No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3330.131211877630148No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC3280.129241729317383No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3260.128453669992277No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA3150.12411934370419404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2970.1170268097782401No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT2710.10678203855186219No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG2700.10638800888930917No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2670.1052059199016502No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA2650.10441786057654419No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT2550.10047756395101425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCACC250.00548837529.612
TAGTAGC250.00548837529.622
CGATGTT358.85028E-426.42857222
GGTATCA1450.025.517241
CCTACGA400.001927065123.1259
TATATAG400.001927065123.1252
ACCAATT400.001927065123.12525
ACGTAGG802.7046553E-823.12527
GGCCTAA400.001927065123.12510
CCCATAG400.001927065123.1251
GTCAACC652.6691887E-622.7692315
GTATCAA4850.022.5051561
AGTACTG502.6940697E-422.25
TCAACCC705.0761137E-621.14285916
TCGATGT450.003817719420.55555721
GAAAGCG450.003817719420.55555734
TTACGAC450.003817719420.55555713
ACGACCT450.003817719420.55555715
AACGGCC555.1279285E-420.18181837
TTAACGG555.1279285E-420.18181835
Produced by FastQC (version 0.11.5)