FastQCFastQC Report
Fri 10 Feb 2017
ERR1632996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413927
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10680.25801651015758814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.1903717322136512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4150.10025922445262088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA250.005492330529.611
CACGAGA405.931088E-527.75000221
CTCGGTA358.859768E-426.42857227
AGGTTCG358.859768E-426.4285728
GCCGGCA601.3339541E-624.66666815
TTCGCCG451.3212144E-424.66666824
GGTATCA2650.024.4339621
CTAGACC400.00192911123.1250023
GGTTCGT400.00192911123.1250029
TCGCCGG652.6759626E-622.7692313
ATATAGC753.728801E-722.26
GTATCAA6200.021.7822571
TAACGGC603.7188744E-521.58333436
AACGGCC705.0889157E-621.14285937
AAAGCGT806.9379894E-720.812535
ATCTCCG450.003821734320.55555710
GTTCGTT450.003821734320.55555710
AACGATT450.003821734320.55555722
TCGGTAG450.003821734320.55555728
GGCCTAA1001.2838427E-820.3510
Produced by FastQC (version 0.11.5)