##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632996.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413927
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58632077636878	33.0	33.0	33.0	27.0	33.0
2	31.123205299485175	33.0	33.0	33.0	27.0	33.0
3	31.56513104967784	33.0	33.0	33.0	27.0	33.0
4	31.714609097739455	33.0	33.0	33.0	27.0	33.0
5	31.82528078622559	33.0	33.0	33.0	33.0	33.0
6	34.688036779432124	37.0	37.0	37.0	27.0	37.0
7	34.84288775557042	37.0	37.0	37.0	27.0	37.0
8	35.00720416885103	37.0	37.0	37.0	33.0	37.0
9	35.10520454089731	37.0	37.0	37.0	33.0	37.0
10	35.13336892737125	37.0	37.0	37.0	33.0	37.0
11	35.15264768908527	37.0	37.0	37.0	33.0	37.0
12	35.11133364095601	37.0	37.0	37.0	33.0	37.0
13	35.13271422255615	37.0	37.0	37.0	33.0	37.0
14	35.11549621068449	37.0	37.0	37.0	33.0	37.0
15	35.133006544632266	37.0	37.0	37.0	33.0	37.0
16	35.1338641837812	37.0	37.0	37.0	33.0	37.0
17	35.08405830013505	37.0	37.0	37.0	33.0	37.0
18	35.10760351462939	37.0	37.0	37.0	33.0	37.0
19	35.09458672664503	37.0	37.0	37.0	33.0	37.0
20	35.10918591925629	37.0	37.0	37.0	33.0	37.0
21	35.10791516378492	37.0	37.0	37.0	33.0	37.0
22	35.117554544641926	37.0	37.0	37.0	33.0	37.0
23	35.11153415940492	37.0	37.0	37.0	33.0	37.0
24	35.10546304058445	37.0	37.0	37.0	33.0	37.0
25	35.08129694366398	37.0	37.0	37.0	33.0	37.0
26	35.060027492770466	37.0	37.0	37.0	33.0	37.0
27	34.98468328956555	37.0	37.0	37.0	27.0	37.0
28	34.94349245156755	37.0	37.0	37.0	27.0	37.0
29	34.99210488805997	37.0	37.0	37.0	27.0	37.0
30	34.945028954380845	37.0	37.0	37.0	27.0	37.0
31	34.9487614965924	37.0	37.0	37.0	27.0	37.0
32	34.98424843027877	37.0	37.0	37.0	27.0	37.0
33	34.97894314698002	37.0	37.0	37.0	27.0	37.0
34	34.88100317205691	37.0	37.0	37.0	27.0	37.0
35	34.902779958785004	37.0	37.0	37.0	27.0	37.0
36	34.83937022711734	37.0	37.0	37.0	27.0	37.0
37	34.888750915016416	37.0	37.0	37.0	27.0	37.0
38	34.747755038931984	37.0	37.0	37.0	27.0	37.0
39	34.8215724028633	37.0	37.0	37.0	27.0	37.0
40	34.84511761735765	37.0	37.0	37.0	27.0	37.0
41	34.87800505886304	37.0	37.0	37.0	27.0	37.0
42	34.787018000758586	37.0	37.0	37.0	27.0	37.0
43	34.57700995586178	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	14.0
17	25.0
18	9.0
19	13.0
20	45.0
21	114.0
22	312.0
23	772.0
24	1408.0
25	2529.0
26	4048.0
27	5988.0
28	8078.0
29	10638.0
30	13662.0
31	17162.0
32	21639.0
33	27815.0
34	38936.0
35	69903.0
36	190807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72505538416194	20.44345983712104	13.353562343118472	25.47792243559855
2	15.7327258188038	22.980139010018675	37.71607070812003	23.571064463057496
3	17.82850599260256	26.499358582551995	30.092021056853017	25.580114367992422
4	12.960981042550982	17.724139763774772	38.7715708325381	30.54330836113614
5	14.229803805985114	36.773633998265396	35.24462042823976	13.751941767509729
6	30.252677404469868	39.2830136714928	17.44051487339555	13.02379405064178
7	26.84652124649999	31.87373619019779	23.632911117177667	17.64683144612456
8	24.541525438060336	36.032440502793975	20.698577285366742	18.727456773778954
9	25.90505089061598	15.275398802204254	20.170464840418724	38.64908546676104
10	14.66973645111336	28.273101295639087	34.21907727691115	22.838084976336408
11	34.28768840882571	23.359674531982694	24.241955707165758	18.11068135202584
12	22.81585883501197	25.367033317469023	30.64429235106673	21.172815496452273
13	28.114619244456147	22.07611487049649	26.0524198711367	23.756846013910664
14	22.139169467079945	20.649051644372072	27.428024748325186	29.783754140222797
15	23.806371654905334	28.72656289635612	23.872083724908016	23.59498172383053
16	23.217620498300427	28.18492149581931	25.081475719148543	23.515982286731717
17	22.521604050955844	27.580225498699047	26.58222343553332	23.31594701481179
18	23.233082161830467	27.352890727108885	27.241760020486705	22.172267090573943
19	24.503837633205855	26.50757259130233	26.954511302717627	22.034078472774183
20	23.941178033807894	26.442826875270274	27.081586849855167	22.53440824106666
21	23.27294426311886	26.83975676870559	26.967073904335788	22.92022506383976
22	23.020725876785036	26.72621017715684	27.20262268467628	23.050441261381838
23	22.875048015712913	26.931318807422567	27.328490289350537	22.865142887513983
24	23.28695639569296	27.016599545330454	26.374215743355713	23.322228315620873
25	23.3843165582337	26.6984275005013	27.24924926375907	22.668006677505936
26	23.242020936058776	26.986400983748343	26.89894594940654	22.872632130786346
27	23.25627465712553	26.76462274748929	26.647452328550685	23.33165026683449
28	22.96564370045926	26.830817994477286	27.18667784416093	23.016860460902528
29	23.52274676452611	26.770904048298373	26.844830127051388	22.861519060124127
30	23.50873463195201	26.633681784469243	26.858359082640177	22.999224500938574
31	23.340589041062795	26.524725374280973	26.940015993158212	23.19466959149802
32	22.675737509270956	26.71268122156805	27.20383062713957	23.40775064202142
33	22.908870404684883	26.633198607483926	27.401208425640274	23.056722562190917
34	23.326818496981353	26.26936633754261	27.231371715302455	23.17244345017358
35	23.078223938037386	26.953544948747	27.03423550529441	22.933995607921204
36	23.6288041128025	26.716305048957906	26.678858832596084	22.976032005643507
37	23.168819622783726	26.411178782732218	26.952578594776373	23.467422999707676
38	23.297344700877208	26.7982035479686	26.804484848777683	23.099966902376508
39	23.023624938696923	26.610006112188866	27.21591005177241	23.150458897341803
40	22.98593713384244	26.913441258965953	27.266160458245054	22.834461148946552
41	23.020967465277696	26.300048076110038	27.259395980450655	23.419588478161607
42	22.840259272770318	26.794821309071402	27.220500233132896	23.144419185025377
43	23.29806946635518	26.091798795439775	27.167109176255718	23.443022561949327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	11.0
2	18.0
3	69.5
4	121.0
5	121.0
6	153.5
7	186.0
8	172.5
9	159.0
10	214.0
11	269.0
12	269.0
13	424.0
14	579.0
15	1065.0
16	1551.0
17	1793.5
18	2036.0
19	2036.0
20	2070.5
21	2105.0
22	2592.0
23	3079.0
24	4145.0
25	5211.0
26	5211.0
27	6873.0
28	8535.0
29	10527.5
30	12520.0
31	14713.0
32	16906.0
33	16906.0
34	19357.5
35	21809.0
36	23681.5
37	25554.0
38	27469.5
39	29385.0
40	29385.0
41	30624.0
42	31863.0
43	32355.0
44	32847.0
45	32388.0
46	31929.0
47	31929.0
48	31249.5
49	30570.0
50	30389.0
51	30208.0
52	28730.0
53	27252.0
54	27252.0
55	26002.5
56	24753.0
57	22247.0
58	19741.0
59	18230.0
60	16719.0
61	16719.0
62	14713.5
63	12708.0
64	10772.0
65	8836.0
66	7497.0
67	6158.0
68	6158.0
69	5091.0
70	4024.0
71	3325.5
72	2627.0
73	2138.5
74	1650.0
75	1650.0
76	1235.0
77	820.0
78	670.0
79	520.0
80	408.0
81	296.0
82	296.0
83	235.0
84	174.0
85	144.5
86	115.0
87	84.0
88	53.0
89	53.0
90	43.0
91	33.0
92	18.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413927.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.58348974936715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.37557629789883	66.6594237865812
2	6.503148730072988	9.700550532960174
3	1.6172926107975087	3.6186998057742974
4	0.7413389584143653	2.211665864228173
5	0.4305184289924267	1.6054783417835152
6	0.26867503476104587	1.2023233020606796
7	0.18781216998412753	0.9805380938372302
8	0.14022821872610838	0.8366967931144575
9	0.09053957761131651	0.6077481892817089
>10	0.5870078138800191	8.173062749647203
>50	0.04095860687077516	2.0789854979245903
>100	0.01625341537546626	1.8748448766320918
>500	3.250683075093252E-4	0.19104846279401116
>1k	3.250683075093252E-4	0.25893370338071564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.25801651015758814	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	788	0.1903717322136512	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.10025922445262088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.8317698531383555E-4	0.0	0.0
2	0.0	0.0	4.8317698531383555E-4	0.0	0.0
3	0.0	0.0	4.8317698531383555E-4	0.0	0.0
4	0.0	0.0	4.8317698531383555E-4	0.0	0.0
5	0.0	0.0	0.0014495309559415066	0.0	0.0
6	0.0	0.0	0.0016911194485984243	2.4158849265691778E-4	0.0
7	0.0	0.0	0.004107004375167602	2.4158849265691778E-4	0.0
8	0.0	0.0	0.005798123823766026	2.4158849265691778E-4	0.0
9	0.0	0.0	0.008214008750335204	9.663539706276711E-4	0.0
10	0.0	0.0	0.00869718573564904	0.001207942463284589	0.0
11	2.4158849265691778E-4	0.0	0.013045778603473559	0.0014495309559415066	0.0
12	2.4158849265691778E-4	0.0	0.014978486544728902	0.0014495309559415066	0.0
13	2.4158849265691778E-4	0.0	0.015461663530042738	0.0014495309559415066	0.0
14	2.4158849265691778E-4	0.0	0.015703252022699656	0.0014495309559415066	0.0
15	2.4158849265691778E-4	0.0	0.01739437147129808	0.0014495309559415066	0.0
16	2.4158849265691778E-4	0.0	0.018602313934582667	0.0014495309559415066	0.0
17	2.4158849265691778E-4	0.0	0.019085490919896503	0.0016911194485984243	0.0
18	2.4158849265691778E-4	0.0	0.01956866790521034	0.0016911194485984243	0.0
19	2.4158849265691778E-4	0.0	0.020051844890524174	0.0016911194485984243	0.0
20	2.4158849265691778E-4	0.0	0.02029343338318109	0.0016911194485984243	0.0
21	2.4158849265691778E-4	0.0	0.021018198861151846	0.0019327079412553422	0.0
22	2.4158849265691778E-4	0.0	0.021501375846465682	0.00217429643391226	0.0
23	2.4158849265691778E-4	0.0	0.021501375846465682	0.00217429643391226	0.0
24	2.4158849265691778E-4	0.0	0.021984552831779518	0.0026574734192260953	0.0
25	2.4158849265691778E-4	0.0	0.021984552831779518	0.0026574734192260953	0.0
26	2.4158849265691778E-4	0.0	0.021984552831779518	0.0026574734192260953	0.0
27	2.4158849265691778E-4	0.0	0.021984552831779518	0.0036238273898537665	0.0
28	4.8317698531383555E-4	0.0	0.021984552831779518	0.00869718573564904	0.0
29	4.8317698531383555E-4	0.0	0.021984552831779518	0.02295090680240719	0.0
30	4.8317698531383555E-4	0.0	0.021984552831779518	0.04421069415621595	0.0
31	4.8317698531383555E-4	0.0	0.021984552831779518	0.10509099430575923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	25	0.0054923305	29.6	11
CACGAGA	40	5.931088E-5	27.750002	21
CTCGGTA	35	8.859768E-4	26.428572	27
AGGTTCG	35	8.859768E-4	26.428572	8
GCCGGCA	60	1.3339541E-6	24.666668	15
TTCGCCG	45	1.3212144E-4	24.666668	24
GGTATCA	265	0.0	24.433962	1
CTAGACC	40	0.001929111	23.125002	3
GGTTCGT	40	0.001929111	23.125002	9
TCGCCGG	65	2.6759626E-6	22.76923	13
ATATAGC	75	3.728801E-7	22.2	6
GTATCAA	620	0.0	21.782257	1
TAACGGC	60	3.7188744E-5	21.583334	36
AACGGCC	70	5.0889157E-6	21.142859	37
AAAGCGT	80	6.9379894E-7	20.8125	35
ATCTCCG	45	0.0038217343	20.555557	10
GTTCGTT	45	0.0038217343	20.555557	10
AACGATT	45	0.0038217343	20.555557	22
TCGGTAG	45	0.0038217343	20.555557	28
GGCCTAA	100	1.2838427E-8	20.35	10
>>END_MODULE