FastQCFastQC Report
Fri 10 Feb 2017
ERR1632995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102744
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18871.8366035972903527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17831.735381141477848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10661.0375301720781749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.46426068675543103No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3040.29588102468270655No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG2880.2803083391730904No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.2705754107295803No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2600.25305613953126216No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2580.2511095538425602No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG2540.24721638246515612No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1970.19173869033714863No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1870.18200576189363857No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1830.17811259051623451No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1620.15767344078486337No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC1570.1528069765631083No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1500.14599392665265123No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1460.1421007552752472No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1430.1391808767421942No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1400.13626099820914117No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC1390.13528770536479015No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT1340.1304212411430351No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1320.1284746554543331No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA1310.12750136260998207No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1280.12458148407692907No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1240.12068831269952504No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1230.11971501985517402No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.11971501985517402No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1220.11874172701082301No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1200.116795141322121No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1200.116795141322121No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1160.11290196994471696No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1150.11192867710036596No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1130.10998209141166394No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1120.10900879856731292No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT1110.10803550572296193No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG1110.10803550572296193No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC1100.10706221287861092No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA1090.1060889200342599No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1060.10316904150120688No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1060.10316904150120688No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA1050.10219574865685586No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1050.10219574865685586No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.10219574865685586No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG1040.10122245581250487No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA1030.10024916296815385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTT200.001834143237.08
TAGATCT200.001834143237.07
TATAGAT251.2258138E-437.05
GGTATCA4000.032.3751
TTATAGA303.5768998E-430.8333344
GGGGCGA303.5768998E-430.83333428
GCGACCT250.00547335529.631
ATAGATC250.00547335529.66
CCTCGGA250.00547335529.635
ACCTCGG250.00547335529.634
TGGGGCG250.00547335529.627
GACATCT250.00547335529.618
GATCTTG250.00547335529.69
GTGACAT250.00547335529.616
ATTATAG358.814286E-426.4285723
TTCATAT358.814286E-426.42857236
CCTGGAT508.986295E-625.90000213
CTGGATT508.986295E-625.90000214
ACGGTAC451.3117857E-424.6666663
GAATCCA551.8808818E-523.54545411
Produced by FastQC (version 0.11.5)