##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632995.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102744
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83592229229931	33.0	33.0	33.0	14.0	33.0
2	29.882445690259285	33.0	33.0	33.0	14.0	33.0
3	30.34585961224013	33.0	33.0	33.0	14.0	33.0
4	30.50562563264035	33.0	33.0	33.0	27.0	33.0
5	30.671951646811493	33.0	33.0	33.0	27.0	33.0
6	32.21148680214903	37.0	33.0	37.0	14.0	37.0
7	32.49511406992136	37.0	33.0	37.0	14.0	37.0
8	32.68841002880947	37.0	33.0	37.0	14.0	37.0
9	32.403400685198164	37.0	33.0	37.0	14.0	37.0
10	32.597562874717745	37.0	33.0	37.0	14.0	37.0
11	32.539496223623765	37.0	33.0	37.0	14.0	37.0
12	32.50269602117885	37.0	33.0	37.0	14.0	37.0
13	32.72447052869267	37.0	33.0	37.0	14.0	37.0
14	32.59203457136183	37.0	33.0	37.0	14.0	37.0
15	32.8325644319863	37.0	33.0	37.0	14.0	37.0
16	32.787637234291054	37.0	33.0	37.0	14.0	37.0
17	32.90669041501207	37.0	33.0	37.0	14.0	37.0
18	32.87803667367437	37.0	33.0	37.0	14.0	37.0
19	32.873462197305926	37.0	33.0	37.0	14.0	37.0
20	32.7491045705832	37.0	33.0	37.0	14.0	37.0
21	32.858356692361596	37.0	33.0	37.0	14.0	37.0
22	32.857393132445694	37.0	33.0	37.0	14.0	37.0
23	32.777125671572065	37.0	33.0	37.0	14.0	37.0
24	32.83002413766254	37.0	33.0	37.0	14.0	37.0
25	32.80314178930156	37.0	33.0	37.0	14.0	37.0
26	32.70195826520283	37.0	33.0	37.0	14.0	37.0
27	32.59625866230631	37.0	33.0	37.0	14.0	37.0
28	32.56744919411352	37.0	33.0	37.0	14.0	37.0
29	32.62878610916452	37.0	33.0	37.0	14.0	37.0
30	32.54611461496535	37.0	33.0	37.0	14.0	37.0
31	32.468552908199015	37.0	33.0	37.0	14.0	37.0
32	32.43969477536401	37.0	33.0	37.0	14.0	37.0
33	32.36434244335435	37.0	33.0	37.0	14.0	37.0
34	32.25506112279063	37.0	33.0	37.0	14.0	37.0
35	32.177888733162035	37.0	27.0	37.0	14.0	37.0
36	32.142373277271666	37.0	27.0	37.0	14.0	37.0
37	32.08586389472865	37.0	27.0	37.0	14.0	37.0
38	31.882066106049987	37.0	27.0	37.0	14.0	37.0
39	31.926691582963482	37.0	27.0	37.0	14.0	37.0
40	31.8468815697267	37.0	27.0	37.0	14.0	37.0
41	31.79103402631784	37.0	27.0	37.0	14.0	37.0
42	31.528167094915517	37.0	27.0	37.0	14.0	37.0
43	31.245406057774662	37.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	8.0
16	12.0
17	14.0
18	22.0
19	54.0
20	121.0
21	357.0
22	651.0
23	1131.0
24	1744.0
25	2520.0
26	3498.0
27	4382.0
28	5216.0
29	6299.0
30	7236.0
31	8396.0
32	9735.0
33	11019.0
34	13082.0
35	15062.0
36	12185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.745191933348906	20.726271120454722	13.490812115549328	21.037724830647043
2	17.74118196683018	27.196721949700226	34.24920189986763	20.812894183601962
3	20.579303900957722	29.01580627579226	29.926808378104802	20.478081445145214
4	13.302966596589583	21.31316670559838	36.865413065483146	28.518453632328892
5	16.3620260063848	36.95787588569649	31.646616834073036	15.033481273845675
6	26.186443977263878	41.798061200654054	15.872459705676246	16.143035116405823
7	25.338705909834154	34.69788990111345	20.865451997196914	19.097952191855487
8	25.858444288717592	33.613641672506425	18.971424122089857	21.556489916686132
9	28.067819045394376	13.12874717745075	18.787471774507512	40.015962002647356
10	17.84727088686444	27.593825430195434	30.580861169508683	23.97804251343144
11	35.42493965584365	23.865140543486724	21.52437125282255	19.185548547847077
12	23.833995172467493	28.30043603519427	27.593825430195434	20.271743362142804
13	31.705987697578447	22.514210075527526	24.77614264579927	21.003659581094762
14	23.250019465856887	22.081094759791327	29.76913493731994	24.899750837031846
15	26.842443354356462	26.34022424667134	26.858016039866072	19.959316359106126
16	20.67565989254847	27.83714864128319	26.985517402476056	24.501674063692285
17	21.52437125282255	25.890562952581174	26.56018842949467	26.024877365101613
18	24.088997897687456	22.67577668768979	29.95308728490228	23.28213812972047
19	27.09355290819902	23.60624464688936	30.203223545900492	19.096978899011134
20	25.699797555088377	22.757533286615278	30.885501829790545	20.657167328505803
21	22.967764540995095	25.41559604453788	30.745347660204004	20.871291754263023
22	22.782838900568404	25.42240909444834	30.63147239741493	21.163279607568324
23	22.21054270809001	25.59565522074282	31.75562563264035	20.438176438526824
24	22.292299307015494	25.633613641672508	30.5467959199564	21.527291131355604
25	22.400334812738457	25.868177217161097	32.33765475356225	19.39383321653819
26	22.50253056139531	25.922681616444756	31.089893327104257	20.484894495055674
27	23.114731760492095	25.109982091411666	31.148290897765317	20.62699525033092
28	21.595421630460173	26.097874328427938	30.911780736588025	21.394923304523868
29	22.30592540683641	25.85941758156194	29.829479093669704	22.00517791793195
30	22.807171221677176	25.528498014482597	31.394533987386126	20.2697967764541
31	22.600833138674766	25.410729580316126	30.49131822782839	21.49711905318072
32	20.275636533520206	25.614147784785484	31.532741571283967	22.57747411041034
33	20.805107840847153	25.71634353344234	31.38674764463132	22.09180098107919
34	21.149653507747413	26.0608892003426	31.095733084170362	21.693724207739624
35	21.461107217939734	26.230242155259674	30.946819278984663	21.361831347815933
36	21.62170053725765	26.454099509460406	30.680137039632484	21.244062913649458
37	22.521996418282335	26.181577513042125	30.014404734096395	21.28202133457915
38	21.314139998442734	26.90181421786187	29.590049053959355	22.193996729736043
39	21.193451685743206	26.298372654364243	29.974499727478005	22.533675932414546
40	21.50685198162423	26.957291909989877	30.758000467180562	20.777855641205324
41	22.39060188429495	25.994705286926727	29.61535466791248	21.999338160865843
42	20.8975706610605	27.759285213735108	29.64455345324301	21.69859067196138
43	22.035349996106827	26.61177295024527	29.16958654519972	22.18329050844818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	34.5
2	26.0
3	83.0
4	140.0
5	140.0
6	217.5
7	295.0
8	333.5
9	372.0
10	486.5
11	601.0
12	601.0
13	962.5
14	1324.0
15	2318.0
16	3312.0
17	3232.0
18	3152.0
19	3152.0
20	2584.5
21	2017.0
22	1439.0
23	861.0
24	785.0
25	709.0
26	709.0
27	721.0
28	733.0
29	950.0
30	1167.0
31	1469.5
32	1772.0
33	1772.0
34	2439.0
35	3106.0
36	3448.5
37	3791.0
38	4299.5
39	4808.0
40	4808.0
41	5770.0
42	6732.0
43	7284.0
44	7836.0
45	8258.0
46	8680.0
47	8680.0
48	9239.5
49	9799.0
50	9856.0
51	9913.0
52	9461.5
53	9010.0
54	9010.0
55	8068.5
56	7127.0
57	6091.0
58	5055.0
59	4583.0
60	4111.0
61	4111.0
62	3356.5
63	2602.0
64	1889.0
65	1176.0
66	1148.5
67	1121.0
68	1121.0
69	871.5
70	622.0
71	479.5
72	337.0
73	320.0
74	303.0
75	303.0
76	185.5
77	68.0
78	38.0
79	8.0
80	6.5
81	5.0
82	5.0
83	6.0
84	7.0
85	4.0
86	1.0
87	1.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	102744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.42875496379351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68939967425963	33.81122011990968
2	7.9209964902621985	6.721560383088064
3	3.3743949716697634	4.295141322120999
4	2.009496937581722	3.4104181266059332
5	1.3098433234693645	2.778751070622129
6	0.9336361342417362	2.3767811259051626
7	0.6193654944601198	1.8395234758234058
8	0.5436652673594384	1.8453632328895118
9	0.43814373867364026	1.6730903994393833
>10	2.79861445644943	22.32636455656778
>50	0.25462803661138256	7.681227127618158
>100	0.10093363613424174	6.6310441485634195
>500	0.0	0.0
>1k	0.006881838827334664	4.609514910846375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1887	1.8366035972903527	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1783	1.735381141477848	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1066	1.0375301720781749	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	477	0.46426068675543103	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	304	0.29588102468270655	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	288	0.2803083391730904	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	278	0.2705754107295803	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	260	0.25305613953126216	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	258	0.2511095538425602	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	254	0.24721638246515612	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	197	0.19173869033714863	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	187	0.18200576189363857	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	183	0.17811259051623451	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	162	0.15767344078486337	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	157	0.1528069765631083	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	150	0.14599392665265123	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	146	0.1421007552752472	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	143	0.1391808767421942	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	140	0.13626099820914117	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	139	0.13528770536479015	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	134	0.1304212411430351	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	132	0.1284746554543331	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	131	0.12750136260998207	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	128	0.12458148407692907	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	124	0.12068831269952504	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	123	0.11971501985517402	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.11971501985517402	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	122	0.11874172701082301	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	120	0.116795141322121	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	120	0.116795141322121	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	116	0.11290196994471696	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	115	0.11192867710036596	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	113	0.10998209141166394	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	112	0.10900879856731292	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	111	0.10803550572296193	No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG	111	0.10803550572296193	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	110	0.10706221287861092	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	109	0.1060889200342599	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	106	0.10316904150120688	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	106	0.10316904150120688	No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA	105	0.10219574865685586	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	105	0.10219574865685586	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.10219574865685586	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	104	0.10122245581250487	No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA	103	0.10024916296815385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0038931713774040333	0.0	0.0
2	0.0	0.0	0.0038931713774040333	0.0	0.0
3	0.0	0.0	0.0038931713774040333	0.0	0.0
4	0.0	0.0	0.0048664642217550414	0.0	0.0
5	0.0	0.0	0.0058397570661060496	0.0	0.0
6	0.0	0.0	0.012652806976563109	0.0	0.0
7	0.0	0.0	0.033091956707934286	0.0	0.0
8	0.0	0.0	0.044771470840146384	0.0	0.0
9	0.0	0.0	0.05645098497235848	9.732928443510083E-4	0.0
10	0.0029198785330530248	0.0	0.06326403488281554	9.732928443510083E-4	0.0
11	0.0029198785330530248	0.0	0.09927587012380285	9.732928443510083E-4	0.0
12	0.0029198785330530248	0.0	0.11874172701082301	9.732928443510083E-4	0.0
13	0.0038931713774040333	0.0	0.1275013626099821	9.732928443510083E-4	0.0
14	0.0038931713774040333	0.0	0.13723429105349216	9.732928443510083E-4	0.0
15	0.0038931713774040333	0.0	0.14502063380830024	9.732928443510083E-4	0.0
16	0.0038931713774040333	0.0	0.1518336837187573	9.732928443510083E-4	0.0
17	0.0038931713774040333	0.0	0.15670014794051235	9.732928443510083E-4	0.0
18	0.0038931713774040333	0.0	0.16059331931791637	9.732928443510083E-4	0.0
19	0.0038931713774040333	0.0	0.1635131978509694	9.732928443510083E-4	0.0
20	0.0038931713774040333	0.0	0.16935295491707544	9.732928443510083E-4	0.0
21	0.0038931713774040333	0.0	0.17713929767188352	9.732928443510083E-4	0.0
22	0.0038931713774040333	0.0	0.17713929767188352	9.732928443510083E-4	0.0
23	0.0038931713774040333	0.0	0.17811259051623451	0.0019465856887020167	0.0
24	0.0038931713774040333	0.0	0.17908588336058553	0.0038931713774040333	0.0
25	0.0038931713774040333	0.0	0.17908588336058553	0.0038931713774040333	0.0
26	0.0038931713774040333	0.0	0.17908588336058553	0.0038931713774040333	0.0
27	0.0038931713774040333	0.0	0.17908588336058553	0.0068130499104570585	0.0
28	0.0038931713774040333	0.0	0.17908588336058553	0.03211866386358327	0.0
29	0.0038931713774040333	0.0	0.17908588336058553	0.08467647745853772	0.0
30	0.0038931713774040333	0.0	0.17908588336058553	0.15280697656310832	0.0
31	0.0038931713774040333	0.0	0.17908588336058553	0.2696021178852293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCTT	20	0.0018341432	37.0	8
TAGATCT	20	0.0018341432	37.0	7
TATAGAT	25	1.2258138E-4	37.0	5
GGTATCA	400	0.0	32.375	1
TTATAGA	30	3.5768998E-4	30.833334	4
GGGGCGA	30	3.5768998E-4	30.833334	28
GCGACCT	25	0.005473355	29.6	31
ATAGATC	25	0.005473355	29.6	6
CCTCGGA	25	0.005473355	29.6	35
ACCTCGG	25	0.005473355	29.6	34
TGGGGCG	25	0.005473355	29.6	27
GACATCT	25	0.005473355	29.6	18
GATCTTG	25	0.005473355	29.6	9
GTGACAT	25	0.005473355	29.6	16
ATTATAG	35	8.814286E-4	26.428572	3
TTCATAT	35	8.814286E-4	26.428572	36
CCTGGAT	50	8.986295E-6	25.900002	13
CTGGATT	50	8.986295E-6	25.900002	14
ACGGTAC	45	1.3117857E-4	24.666666	3
GAATCCA	55	1.8808818E-5	23.545454	11
>>END_MODULE