FastQCFastQC Report
Fri 10 Feb 2017
ERR1632994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366760
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8670.23639437234158578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.1785909041334933No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG3830.10442796379103501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3750.10224670083978622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC1450.026.7931023
CTAGACA1500.025.94
GGTATCA1750.025.3714281
GTCTAGA1400.022.4642851
GTATCAA4700.022.436171
TTAACCT753.726418E-722.24
AGACATG1450.021.6896556
TAGACAT1800.021.5833345
CCTACAT603.717387E-521.5833343
TTAGACA450.003820916420.5555574
CTAGAAC1450.020.4137943
GACATGT1450.020.4137947
TAGAACT1650.020.1818184
GCTCGGA555.133922E-420.18181811
GTTCTAG1900.019.4736841
CTCTAGT1900.019.47368427
CAATTAC1355.4569682E-1119.18518618
TTTGAAC2050.018.95121835
ACAACGG1205.138645E-918.523
GACGTTA500.007025972518.520
Produced by FastQC (version 0.11.5)