##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632994.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366760
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65600937943069	33.0	33.0	33.0	27.0	33.0
2	31.17497818737049	33.0	33.0	33.0	27.0	33.0
3	31.634976551423275	33.0	33.0	33.0	27.0	33.0
4	31.76243319882212	33.0	33.0	33.0	27.0	33.0
5	31.881137528629075	33.0	33.0	33.0	33.0	33.0
6	34.7673982986149	37.0	37.0	37.0	27.0	37.0
7	34.91133166103174	37.0	37.0	37.0	27.0	37.0
8	35.08406042098375	37.0	37.0	37.0	33.0	37.0
9	35.18521649034791	37.0	37.0	37.0	33.0	37.0
10	35.22887992147454	37.0	37.0	37.0	33.0	37.0
11	35.248006870978294	37.0	37.0	37.0	33.0	37.0
12	35.20832969789508	37.0	37.0	37.0	33.0	37.0
13	35.22575798887556	37.0	37.0	37.0	33.0	37.0
14	35.20739720798342	37.0	37.0	37.0	33.0	37.0
15	35.221436361653396	37.0	37.0	37.0	33.0	37.0
16	35.2272685134693	37.0	37.0	37.0	33.0	37.0
17	35.19942469189661	37.0	37.0	37.0	33.0	37.0
18	35.19495037626786	37.0	37.0	37.0	33.0	37.0
19	35.20194132402661	37.0	37.0	37.0	33.0	37.0
20	35.20697458828662	37.0	37.0	37.0	33.0	37.0
21	35.2040135238303	37.0	37.0	37.0	33.0	37.0
22	35.22040298833024	37.0	37.0	37.0	33.0	37.0
23	35.20665557858	37.0	37.0	37.0	33.0	37.0
24	35.198511288035775	37.0	37.0	37.0	33.0	37.0
25	35.19370160322827	37.0	37.0	37.0	33.0	37.0
26	35.155916675755265	37.0	37.0	37.0	33.0	37.0
27	35.088611080815795	37.0	37.0	37.0	33.0	37.0
28	35.063223906641944	37.0	37.0	37.0	27.0	37.0
29	35.10483967717308	37.0	37.0	37.0	33.0	37.0
30	35.07735031082997	37.0	37.0	37.0	33.0	37.0
31	35.059692987239615	37.0	37.0	37.0	27.0	37.0
32	35.09351619587741	37.0	37.0	37.0	33.0	37.0
33	35.07487457738031	37.0	37.0	37.0	33.0	37.0
34	34.99668993347148	37.0	37.0	37.0	27.0	37.0
35	34.997892354673354	37.0	37.0	37.0	27.0	37.0
36	34.986339840767805	37.0	37.0	37.0	27.0	37.0
37	35.00680281382921	37.0	37.0	37.0	27.0	37.0
38	34.8682462645872	37.0	37.0	37.0	27.0	37.0
39	34.943941542152906	37.0	37.0	37.0	27.0	37.0
40	34.96942959973825	37.0	37.0	37.0	27.0	37.0
41	34.994328716326756	37.0	37.0	37.0	27.0	37.0
42	34.903811757007304	37.0	37.0	37.0	27.0	37.0
43	34.72474915476061	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	16.0
17	13.0
18	15.0
19	14.0
20	26.0
21	86.0
22	232.0
23	533.0
24	1031.0
25	2055.0
26	3251.0
27	4707.0
28	6763.0
29	8825.0
30	11496.0
31	14788.0
32	18394.0
33	23990.0
34	33897.0
35	61365.0
36	175250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.455447704220745	20.16059548478569	13.084033155196858	25.299923655796707
2	16.262678590904134	22.965699640091614	37.09646635401897	23.675155414985277
3	18.459210382811648	25.393990620569312	30.282473552186715	25.86432544443233
4	13.146744465045263	17.197622423383137	38.575635292834555	31.07999781873705
5	14.284545751990402	36.79108954084415	35.21130984840222	13.713054858763224
6	31.023830297742393	39.11986039917112	16.656669211473442	13.199640091613043
7	27.388482931617407	31.64439960737267	22.98151379648817	17.985603664521758
8	25.1387828552732	35.60502781110263	20.506325662558623	18.749863671065548
9	25.826698658523284	14.901843167193803	20.084251281491984	39.187206892790925
10	14.43341694841313	28.57290871414549	34.06123895735631	22.93243538008507
11	34.432326316937505	23.180008725051803	24.1280401352383	18.259624822772384
12	23.170738357508995	25.112880357727125	30.66555785799978	21.050823426764094
13	28.32833460573672	21.919238739230014	26.41563965536045	23.33678699967281
14	22.463463845566583	20.427254880575855	26.917330134147672	30.191951139709893
15	23.77631148434944	29.07596248227724	23.844203293707057	23.303522739666267
16	23.490020721998036	27.988057585341913	24.68071763551096	23.841204057149092
17	22.464554477042206	27.423928454575197	26.3774675537136	23.734049514668992
18	23.65116152252154	26.75618933362417	27.251608681426546	22.341040462427745
19	24.759243101755917	26.047824190206132	26.87479550659832	22.318137201439633
20	24.07923437670411	26.847529719707712	26.68120841967499	22.392027483913186
21	23.388864652633874	26.939142763660158	27.247791471261863	22.424201112444106
22	23.19718617079289	26.367379212564074	27.241792998145925	23.19364161849711
23	23.22636056276584	26.674664630821244	26.91596684480314	23.18300796160977
24	23.148653070127605	26.762733122477915	26.340931399280183	23.747682408114297
25	23.924910022903262	26.985767259243104	26.58005235031083	22.509270367542808
26	24.022521539971645	26.2970334823863	26.49607372668775	23.184371250954303
27	23.421583596902607	26.756461991493076	26.490893227178535	23.331061184425785
28	23.044497764205474	26.30903042861817	27.36176246046461	23.284709346711747
29	23.4346711746101	26.41836623404952	26.457901625040897	23.68906096629949
30	23.08403315519686	26.870432980695824	27.167902715672376	22.877631148434943
31	23.5958119751336	26.527429381611956	26.995037626785912	22.881721016468536
32	23.394863125749808	26.47235249209292	27.03729959646635	23.095484785690914
33	23.462209619369613	26.31611953320973	27.272603337332313	22.94906751008834
34	23.8371141891155	26.045915585123787	26.73301341476715	23.383956810993563
35	23.044497764205474	26.968044497764204	27.079561566146797	22.90789617188352
36	23.770313011233505	26.027920165775985	27.272603337332313	22.929163485658197
37	23.622259788417495	26.079452502999235	26.881884611189875	23.416403097393392
38	23.652524811866073	26.360562765841422	27.10682735303741	22.8800850692551
39	23.53391863889192	26.506980041443995	27.02039480859418	22.93870651106991
40	23.201276038826478	26.966681208419672	27.189442687315957	22.642600065437886
41	24.077871087359583	25.77652961064456	26.926327843821575	23.219271458174283
42	23.289071872614244	26.565056167520996	26.596957138183008	23.548914821681755
43	23.148653070127605	26.129076235140147	27.278329152579346	23.443941542152906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	10.5
2	17.0
3	71.0
4	125.0
5	125.0
6	134.5
7	144.0
8	140.5
9	137.0
10	183.0
11	229.0
12	229.0
13	338.0
14	447.0
15	862.0
16	1277.0
17	1500.5
18	1724.0
19	1724.0
20	1794.0
21	1864.0
22	2264.5
23	2665.0
24	3584.5
25	4504.0
26	4504.0
27	5761.5
28	7019.0
29	8565.5
30	10112.0
31	11826.0
32	13540.0
33	13540.0
34	15828.0
35	18116.0
36	20101.0
37	22086.0
38	23320.0
39	24554.0
40	24554.0
41	25784.5
42	27015.0
43	28045.5
44	29076.0
45	29638.0
46	30200.0
47	30200.0
48	28985.5
49	27771.0
50	27793.5
51	27816.0
52	26796.5
53	25777.0
54	25777.0
55	24073.0
56	22369.0
57	19951.0
58	17533.0
59	16787.5
60	16042.0
61	16042.0
62	14722.0
63	13402.0
64	10754.5
65	8107.0
66	6737.0
67	5367.0
68	5367.0
69	4275.0
70	3183.0
71	2573.0
72	1963.0
73	1577.5
74	1192.0
75	1192.0
76	882.0
77	572.0
78	457.0
79	342.0
80	263.5
81	185.0
82	185.0
83	151.5
84	118.0
85	102.5
86	87.0
87	64.0
88	41.0
89	41.0
90	36.5
91	32.0
92	18.5
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	366760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.43354487956977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.70664295169428	56.51249914864395
2	6.5951873934955465	8.49902605815936
3	1.917109926240334	3.705785654144257
4	0.8986124206599752	2.316031349437333
5	0.5574305747297814	1.7958613977047873
6	0.3899848229475416	1.5076862755044895
7	0.27104468300573287	1.2225058822773054
8	0.1999044272628963	1.0304440708534814
9	0.16759413596068223	0.97188158528778
>10	1.170734931696206	14.606069565074876
>50	0.09548757978572153	4.142569746899494
>100	0.02941358484464511	3.2715918482453277
>500	8.525676766563801E-4	0.41804741776755844
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.23639437234158578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.1785909041334933	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	383	0.10442796379103501	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.10224670083978622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7265786890609664E-4	0.0
3	0.0	0.0	0.0	5.453157378121933E-4	0.0
4	0.0	0.0	0.0	5.453157378121933E-4	0.0
5	0.0	0.0	2.7265786890609664E-4	5.453157378121933E-4	0.0
6	0.0	0.0	0.0019086050823426764	5.453157378121933E-4	0.0
7	0.0	0.0	0.0032718944268731597	5.453157378121933E-4	0.0
8	0.0	0.0	0.004089868033591449	5.453157378121933E-4	0.0
9	0.0	0.0	0.004362525902497546	0.001363289344530483	0.0
10	0.0	0.0	0.005180499509215836	0.001363289344530483	0.0
11	0.0	0.0	0.007089104591558512	0.001363289344530483	0.0
12	0.0	0.0	0.007907078198276803	0.001363289344530483	0.0
13	0.0	0.0	0.007907078198276803	0.001363289344530483	0.0
14	0.0	0.0	0.007907078198276803	0.001363289344530483	0.0
15	0.0	0.0	0.008725051804995092	0.001363289344530483	0.0
16	0.0	0.0	0.008725051804995092	0.001363289344530483	0.0
17	0.0	0.0	0.008725051804995092	0.0016359472134365798	0.0
18	0.0	0.0	0.008725051804995092	0.0016359472134365798	0.0
19	0.0	0.0	0.008725051804995092	0.0016359472134365798	0.0
20	0.0	0.0	0.008725051804995092	0.0016359472134365798	0.0
21	0.0	0.0	0.00899770967390119	0.002181262951248773	0.0
22	0.0	0.0	0.00899770967390119	0.002726578689060966	0.0
23	0.0	0.0	0.00899770967390119	0.002726578689060966	0.0
24	0.0	0.0	0.00899770967390119	0.002999236557967063	0.0
25	0.0	0.0	0.00899770967390119	0.002999236557967063	0.0
26	0.0	0.0	0.00899770967390119	0.002999236557967063	0.0
27	0.0	0.0	0.00899770967390119	0.004362525902497546	0.0
28	0.0	0.0	0.00899770967390119	0.008725051804995092	0.0
29	0.0	0.0	0.00899770967390119	0.01881339295452067	0.0
30	0.0	0.0	0.00899770967390119	0.035718180826698656	0.0
31	0.0	0.0	0.00899770967390119	0.08234267640964119	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	145	0.0	26.793102	3
CTAGACA	150	0.0	25.9	4
GGTATCA	175	0.0	25.371428	1
GTCTAGA	140	0.0	22.464285	1
GTATCAA	470	0.0	22.43617	1
TTAACCT	75	3.726418E-7	22.2	4
AGACATG	145	0.0	21.689655	6
TAGACAT	180	0.0	21.583334	5
CCTACAT	60	3.717387E-5	21.583334	3
TTAGACA	45	0.0038209164	20.555557	4
CTAGAAC	145	0.0	20.413794	3
GACATGT	145	0.0	20.413794	7
TAGAACT	165	0.0	20.181818	4
GCTCGGA	55	5.133922E-4	20.181818	11
GTTCTAG	190	0.0	19.473684	1
CTCTAGT	190	0.0	19.473684	27
CAATTAC	135	5.4569682E-11	19.185186	18
TTTGAAC	205	0.0	18.951218	35
ACAACGG	120	5.138645E-9	18.5	23
GACGTTA	50	0.0070259725	18.5	20
>>END_MODULE