FastQCFastQC Report
Fri 10 Feb 2017
ERR1632993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320955
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11970.37294947889891106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.2791668613980153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4900.1526693773270396No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT4280.13335202754280195No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG3340.10406443270863516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG200.001839959937.03
GTATCAA6350.025.0551191
GGTATCA2150.024.0930231
GCGTTTA450.003819891320.55555725
CTAATAT450.003819891320.5555573
GGGTGTA500.00702410718.57
TATTGTC1409.276846E-1118.55
ACAATGC500.00702410718.58
CAAGACT500.00702410718.54
ATCAACG8600.017.854653
TCAACGC8700.017.6494244
TATCAAC8750.017.5485732
CTAACAC953.5900266E-617.5263163
CCGGAGT752.0614223E-417.26666616
GGAGTCG752.0614223E-417.26666618
CGGAGTC752.0614223E-417.26666617
CAACGCA8800.017.2386365
CGAACTA650.001576582217.07692329
GTCTATA650.001576582217.0769231
ATAGTGG650.001576582217.0769236
Produced by FastQC (version 0.11.5)