##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632993.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320955
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71234908320481	33.0	33.0	33.0	27.0	33.0
2	31.218463647551836	33.0	33.0	33.0	27.0	33.0
3	31.66978236824477	33.0	33.0	33.0	27.0	33.0
4	31.80407533766416	33.0	33.0	33.0	27.0	33.0
5	31.900852144381613	33.0	33.0	33.0	33.0	33.0
6	34.8055490645106	37.0	37.0	37.0	27.0	37.0
7	34.961446308672556	37.0	37.0	37.0	27.0	37.0
8	35.13563895250113	37.0	37.0	37.0	33.0	37.0
9	35.206349799816174	37.0	37.0	37.0	33.0	37.0
10	35.228885669330595	37.0	37.0	37.0	33.0	37.0
11	35.266295898178875	37.0	37.0	37.0	33.0	37.0
12	35.21049368291505	37.0	37.0	37.0	33.0	37.0
13	35.25306039787509	37.0	37.0	37.0	33.0	37.0
14	35.21573429296942	37.0	37.0	37.0	33.0	37.0
15	35.23532894019411	37.0	37.0	37.0	33.0	37.0
16	35.223660637784114	37.0	37.0	37.0	33.0	37.0
17	35.18960601953545	37.0	37.0	37.0	33.0	37.0
18	35.19752613294698	37.0	37.0	37.0	33.0	37.0
19	35.21317941767538	37.0	37.0	37.0	33.0	37.0
20	35.227066099608976	37.0	37.0	37.0	33.0	37.0
21	35.22452680282282	37.0	37.0	37.0	33.0	37.0
22	35.22557056285149	37.0	37.0	37.0	33.0	37.0
23	35.19282453926563	37.0	37.0	37.0	33.0	37.0
24	35.20564565125952	37.0	37.0	37.0	33.0	37.0
25	35.192129737813715	37.0	37.0	37.0	33.0	37.0
26	35.157573491610975	37.0	37.0	37.0	33.0	37.0
27	35.105257746413045	37.0	37.0	37.0	33.0	37.0
28	35.0665763113209	37.0	37.0	37.0	33.0	37.0
29	35.09977099593401	37.0	37.0	37.0	33.0	37.0
30	35.061843560623764	37.0	37.0	37.0	27.0	37.0
31	35.05808602452057	37.0	37.0	37.0	27.0	37.0
32	35.07910766306803	37.0	37.0	37.0	33.0	37.0
33	35.060076957828976	37.0	37.0	37.0	27.0	37.0
34	34.99074325061146	37.0	37.0	37.0	27.0	37.0
35	34.993326167219706	37.0	37.0	37.0	27.0	37.0
36	34.97905936969357	37.0	37.0	37.0	27.0	37.0
37	34.98406007072643	37.0	37.0	37.0	27.0	37.0
38	34.85047124986369	37.0	37.0	37.0	27.0	37.0
39	34.91680142075992	37.0	37.0	37.0	27.0	37.0
40	34.95854559050334	37.0	37.0	37.0	27.0	37.0
41	34.96449658051752	37.0	37.0	37.0	27.0	37.0
42	34.87217522705675	37.0	37.0	37.0	27.0	37.0
43	34.66084965182035	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	17.0
17	20.0
18	18.0
19	15.0
20	32.0
21	83.0
22	203.0
23	449.0
24	957.0
25	1694.0
26	2804.0
27	4240.0
28	5856.0
29	7798.0
30	9872.0
31	12769.0
32	15962.0
33	21158.0
34	29665.0
35	53650.0
36	153689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.332756928541386	21.092676543440668	14.007259584676978	24.567306943340967
2	15.192472464987302	23.733856771198454	38.93193749902634	22.1417332647879
3	17.30834540667695	27.31691358601673	31.128039756352138	24.24670125095418
4	12.314498917293703	18.287921982832483	40.28446355408079	29.113115545793022
5	13.181910236637535	37.40119331370441	36.290134130952936	13.126762318705115
6	28.48623638827873	40.688881618918536	17.962331167920738	12.862550824881994
7	25.43253727158013	32.7718839089592	24.39500864607188	17.400570173388793
8	23.67683943231917	36.54125967814802	21.65225654686794	18.12964434266486
9	25.749404122073187	15.653284728388714	20.958701375582248	37.63860977395585
10	14.080478571762397	28.829898272343474	35.18873362309358	21.900889532800548
11	32.7083235967659	24.377249147076693	24.872645697995047	18.04178155816236
12	21.701484631801964	26.180305650324815	31.860229627206305	20.257980090666916
13	27.545606081849485	22.86457603090776	27.285133429920084	22.304684457322676
14	21.658799520181958	21.632627626925892	28.711190042217755	27.99738281067439
15	22.91006527394806	29.513483198579237	25.104765465563712	22.47168606190899
16	22.11026467884906	29.45926999112025	25.923260270131326	22.507205059899363
17	21.314202925643784	28.466607468336687	27.712607686435792	22.50658191958374
18	22.271658020594785	28.143820784845225	28.542630586842392	21.04189060771759
19	23.767506348241966	26.986337648580015	28.476577713386614	20.769578289791404
20	23.483665934476797	27.13028306148837	28.2793538034927	21.106697200542133
21	22.40719103924226	27.582371360471093	27.81729525946005	22.193142340826597
22	21.766602794784315	27.720396940381047	28.169681107943482	22.343319156891152
23	21.73856148058139	27.63378043650979	28.731130532317618	21.896527550591205
24	21.938277951737785	27.6976523188609	28.184013335202756	22.180056394198562
25	22.168528298359583	27.62162920035519	28.327958748111104	21.881883753174122
26	22.08970104843358	27.861226651711302	28.33917527379228	21.709897026062844
27	22.40750260940007	27.773675437366606	27.581748220155472	22.237073733077846
28	22.05480519075883	27.66867629418454	28.38653393777944	21.88998457727719
29	22.331167920736554	27.5917184652054	28.154102600052965	21.923011014005077
30	22.162296895203376	27.803274602358584	28.302409995170663	21.732018507267373
31	22.215575392188935	27.75093081584646	28.069667087286383	21.963826704678226
32	21.412970665669643	27.52971600380116	28.54979670047203	22.50751663005717
33	21.738873050739198	27.606985402938104	28.721160287267683	21.932981259055005
34	22.24984810954807	27.241513607826644	28.351949650262497	22.15668863236279
35	22.057609322179122	27.91637456964372	28.34197940521257	21.68403670296459
36	22.066333286597807	27.786449813836832	28.318923213534607	21.828293686030754
37	22.29720677353523	27.259584676979635	28.198033992304218	22.245174557180913
38	22.411864591609415	27.365206960477323	28.298359583119133	21.924568864794132
39	22.05573990123226	27.443722640245515	28.154414170210778	22.346123288311446
40	21.7176862800081	27.865900204078454	28.54356529731582	21.872848218597625
41	21.767225935099933	27.07918555560748	28.801545387982742	22.35204312130984
42	22.028321727344956	27.38234331915689	28.26377529560219	22.325559657895965
43	21.927684566372232	26.896293872972848	28.861055288124504	22.314966272530416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	18.5
2	24.0
3	85.0
4	146.0
5	146.0
6	178.5
7	211.0
8	200.0
9	189.0
10	256.0
11	323.0
12	323.0
13	511.0
14	699.0
15	1243.0
16	1787.0
17	2193.0
18	2599.0
19	2599.0
20	2827.5
21	3056.0
22	3721.5
23	4387.0
24	5683.0
25	6979.0
26	6979.0
27	8450.0
28	9921.0
29	11539.0
30	13157.0
31	15104.5
32	17052.0
33	17052.0
34	18264.5
35	19477.0
36	20531.5
37	21586.0
38	22506.0
39	23426.0
40	23426.0
41	23497.5
42	23569.0
43	23404.0
44	23239.0
45	22723.0
46	22207.0
47	22207.0
48	21361.0
49	20515.0
50	20581.0
51	20647.0
52	20657.0
53	20667.0
54	20667.0
55	19007.0
56	17347.0
57	15720.5
58	14094.0
59	12238.0
60	10382.0
61	10382.0
62	9337.5
63	8293.0
64	6843.5
65	5394.0
66	4466.0
67	3538.0
68	3538.0
69	2883.5
70	2229.0
71	1885.5
72	1542.0
73	1317.0
74	1092.0
75	1092.0
76	802.0
77	512.0
78	396.5
79	281.0
80	221.5
81	162.0
82	162.0
83	123.0
84	84.0
85	78.0
86	72.0
87	52.0
88	32.0
89	32.0
90	26.0
91	20.0
92	11.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	320955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.87520648808082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.7726579257079	68.71496712147386
2	4.83631610903348	7.242403346110268
3	1.202247235188436	2.7005552995337516
4	0.5656061007717316	1.693994943448066
5	0.32621498227567347	1.2212707078698337
6	0.22624897167047958	1.0164263082925862
7	0.14813903815361185	0.7764358749488158
8	0.11814118932620583	0.707667675643769
9	0.08169325098821834	0.5505119132782914
>10	0.6486886418565487	9.567772241685041
>50	0.05187442273130278	2.6938169173966804
>100	0.021335448058827918	2.4585777889111213
>500	4.183421188005474E-4	0.2806581346495559
>1k	4.183421188005474E-4	0.3749417267583911
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.37294947889891106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.2791668613980153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	490	0.1526693773270396	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	428	0.13335202754280195	No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG	334	0.10406443270863516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.115701578102849E-4	0.0	0.0
2	0.0	0.0	6.231403156205698E-4	0.0	0.0
3	0.0	0.0	6.231403156205698E-4	0.0	0.0
4	0.0	0.0	6.231403156205698E-4	0.0	0.0
5	0.0	0.0	6.231403156205698E-4	0.0	0.0
6	0.0	0.0	0.0012462806312411397	0.0	0.0
7	0.0	0.0	0.003738841893723419	0.0	0.0
8	0.0	0.0	0.004361982209343989	0.0	0.0
9	0.0	0.0	0.005296692682774844	0.0	0.0
10	3.115701578102849E-4	0.0	0.005919832998395414	0.0	0.0
11	6.231403156205698E-4	0.0	0.007789253945257123	0.0	0.0
12	6.231403156205698E-4	0.0	0.008100824103067408	3.115701578102849E-4	0.0
13	6.231403156205698E-4	0.0	0.009035534576498264	3.115701578102849E-4	0.0
14	6.231403156205698E-4	0.0	0.010281815207739404	3.115701578102849E-4	0.0
15	6.231403156205698E-4	0.0	0.011528095838980542	3.115701578102849E-4	0.0
16	6.231403156205698E-4	0.0	0.012462806312411397	3.115701578102849E-4	0.0
17	6.231403156205698E-4	0.0	0.013085946628031968	3.115701578102849E-4	0.0
18	6.231403156205698E-4	0.0	0.013397516785842252	3.115701578102849E-4	0.0
19	6.231403156205698E-4	0.0	0.013397516785842252	3.115701578102849E-4	0.0
20	6.231403156205698E-4	0.0	0.014020657101462821	3.115701578102849E-4	0.0
21	6.231403156205698E-4	0.0	0.014332227259273108	6.231403156205698E-4	0.0
22	6.231403156205698E-4	0.0	0.015266937732703961	6.231403156205698E-4	0.0
23	6.231403156205698E-4	0.0	0.015266937732703961	9.347104734308548E-4	0.0
24	6.231403156205698E-4	0.0	0.015578507890514246	0.0012462806312411397	0.0
25	6.231403156205698E-4	0.0	0.01589007804832453	0.0015578507890514246	0.0
26	6.231403156205698E-4	0.0	0.01589007804832453	0.0018694209468617096	0.0
27	6.231403156205698E-4	0.0	0.01589007804832453	0.0018694209468617096	0.0
28	6.231403156205698E-4	0.0	0.01589007804832453	0.008412394260877693	0.0
29	6.231403156205698E-4	0.0	0.01589007804832453	0.019317349784237665	0.0
30	6.231403156205698E-4	0.0	0.01589007804832453	0.0414388309887679	0.0
31	6.231403156205698E-4	0.0	0.01589007804832453	0.09783302955242947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	20	0.0018399599	37.0	3
GTATCAA	635	0.0	25.055119	1
GGTATCA	215	0.0	24.093023	1
GCGTTTA	45	0.0038198913	20.555557	25
CTAATAT	45	0.0038198913	20.555557	3
GGGTGTA	50	0.007024107	18.5	7
TATTGTC	140	9.276846E-11	18.5	5
ACAATGC	50	0.007024107	18.5	8
CAAGACT	50	0.007024107	18.5	4
ATCAACG	860	0.0	17.85465	3
TCAACGC	870	0.0	17.649424	4
TATCAAC	875	0.0	17.548573	2
CTAACAC	95	3.5900266E-6	17.526316	3
CCGGAGT	75	2.0614223E-4	17.266666	16
GGAGTCG	75	2.0614223E-4	17.266666	18
CGGAGTC	75	2.0614223E-4	17.266666	17
CAACGCA	880	0.0	17.238636	5
CGAACTA	65	0.0015765822	17.076923	29
GTCTATA	65	0.0015765822	17.076923	1
ATAGTGG	65	0.0015765822	17.076923	6
>>END_MODULE