FastQCFastQC Report
Fri 10 Feb 2017
ERR1632992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370001
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116083.1372888181383294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90012.432696127848303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45001.2162129291542456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.6870251702022427No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16040.43351234185853554No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14750.3986475712227805No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14020.3789178948165005No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12800.3459450109594298No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.3183775178986003No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11750.31756670927916414No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11340.30648565814686984No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9260.25026959386596254No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9100.24594528122896964No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8710.23540476917629952No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8680.23459396055686335No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8010.21648590138945567No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.2140534755311472No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7810.21108051059321462No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7250.19594541636373955No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7090.19162110372674668No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7060.19081029510731054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.18756706062956588No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6920.18702652154994176No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6880.18594544339069352No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.18405355661200914No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6630.17918870489539218No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6610.17864816581576806No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6460.17459412271858726No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6450.1743238531787752No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6420.17351304455933902No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6370.17216169686027877No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6120.1654049583649774No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6110.16513468882516533No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5780.15621579401136754No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5720.1545941767724952No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5690.15378336815305904No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5610.15162121183456262No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5600.15135094229475055No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5490.14837797735681796No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5480.1481077078170059No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5470.14783743827719384No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5440.14702662965775767No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5420.14648609057813358No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5280.1427023170207648No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5040.1362158480652755No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4910.13270234404771877No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4870.13162126588847056No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4780.12918884003016207No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4600.1243239883135451No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4470.1208104842959884No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4440.11999967567655223No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4410.11918886705711605No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4350.11756724981824374No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4300.11621590211918346No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4300.11621590211918346No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4290.11594563257937142No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4210.11378347626087497No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.11351320672106291No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4190.11324293718125086No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4090.11054024178313031No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4090.11054024178313031No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4060.10972943316369416No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4040.10918889408407004No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3990.10783754638500978No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3960.1070267377655736No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3930.10621592914613744No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3920.10594565960632539No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3890.10513485098688923No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3840.10378350328782895No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3750.10135107742952046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG200.001840323337.01
TGTCGGG451.07660526E-732.88888523
GACCGTT303.5948036E-430.8333347
CGTCTTC250.00549158629.612
TATGCGC250.00549158629.62
GAGTTGT250.00549158629.632
GGTATCA14550.029.498281
TCGGGTA453.998608E-628.77777525
AAGTTCC453.998608E-628.77777531
GTCGGGT453.998608E-628.77777524
TTAGGAC358.857986E-426.428573
GTCAATT358.857986E-426.4285726
CTTAGGA358.857986E-426.428572
GCCGCTC3100.026.25806427
GTTCCGA509.069348E-625.90000233
GGCAGCT3150.025.8412718
TTCGCCG3150.025.8412724
CCGCTCT3100.025.66129128
GCAGCTT3250.025.61538519
ATTATAG659.35579E-825.6153853
Produced by FastQC (version 0.11.5)