FastQCFastQC Report
Fri 10 Feb 2017
ERR1632991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528035
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48440.9173634323482345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.6626454685768935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18570.35168123325158374No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17540.332174950524113No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17410.3297129925099662No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15800.2992225894116867No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13910.2634295075137065No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13360.25301353130000853No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12620.2389993087579422No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11660.22081869573039667No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11570.21911426325906427No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11370.2153266355449923No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11050.20926643120247712No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10940.20718323595973753No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10890.2062363290312195No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10820.20491065933129432No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10530.19941859914588997No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9960.18862386016078478No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9940.18824509738937759No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9820.1859725207609344No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9700.18369994413249122No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9640.18256365581826964No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9620.18218489304686242No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9330.17669283286145804No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9160.17347334930449687No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9070.17176891683316448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.16760252634768527No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8740.16551933110494568No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8680.1643830427907241No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8630.1634361358622061No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8510.1611635592337629No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8430.1596485081481341No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8310.15737593151969093No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8110.15358830380561894No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7840.14847500639162178No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7750.14677057392028936No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7700.14582366699177138No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7620.14430861590614258No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7560.143172327591921No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7470.1414678951205886No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7450.14108913234918138No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7430.14071036957777422No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7370.1395740812635526No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7360.13938469987784902No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7350.1391953184921454No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7340.1390059371064418No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7340.1390059371064418No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7290.1380590301779238No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7210.13654397909229501No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7190.13616521632088782No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7090.13427140246385183No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6990.13237758860681584No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6990.13237758860681584No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT6960.13180944444970505No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6840.12953686782126186No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6820.12915810504985464No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6790.12858996089274385No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6580.12461295179296827No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA6510.12328728209304307No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6500.12309790070733947No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6490.12290851932163586No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6280.1189315102218603No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6210.1176058405219351No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6190.11722707775052789No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6180.1170376963648243No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6150.1164695522077135No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6030.11419697557927032No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG6010.11381821280786313No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5990.11343945003645592No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5940.11249254310793791No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5850.11078811063660553No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC5760.10908367816527315No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5720.10832615262245875No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5690.10775800846534794No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5670.10737924569394074No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT5660.10718986430823714No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5620.10643233876542275No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5470.10359161797986877No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC5450.10321285520846156No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5380.10188718550853636No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5380.10188718550853636No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5370.10169780412283276No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5340.10112965996572199No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5320.10075089719431478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG200.001841035337.025
TATGCGT250.00549368629.631
GGACGTA405.9341426E-527.7527
GGCAGCT1550.027.45161418
GGTATCA8500.026.9882351
CCGGCAG1650.026.9090916
GCCGGCA1650.026.9090915
CGCTCTC1650.026.9090929
CCGGCGG358.8630203E-426.4285729
GAATCAA358.8630203E-426.428571
CCGCTCT1750.025.3714328
GCCGCTC1750.025.3714327
GCTCTCT1700.025.02941130
GGGTCGC451.3218887E-424.66666815
GACGTAT451.3218887E-424.66666828
TGGGTCG451.3218887E-424.66666814
TTGTCTG601.33512E-624.6666689
ATTGTCT451.3218887E-424.6666688
TAGATAC451.3218887E-424.6666685
ACGTATA451.3218887E-424.66666829
Produced by FastQC (version 0.11.5)