FastQCFastQC Report
Fri 10 Feb 2017
ERR1632990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218898
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5670.2590247512540087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.19689535765516358No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG2740.12517245475061445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2660.12151778453891766No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG2630.12014728320953137No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT2200.10050343082166123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA200.00183868237.05
GTGTCGA250.005486746329.59999813
GGTATCA905.456968E-1226.7222211
TGTCGAA358.846375E-426.4285714
CCCACAC601.3291574E-624.6666683
GCTCTAG551.8935485E-523.5454561
GTATCAA3100.022.677421
GACGTAT502.6924285E-422.19999926
ACGGGAG502.6924285E-422.1999995
ACGTATG502.6924285E-422.19999927
ACCCCAC705.0708477E-621.1428571
TAATAGT450.003816066520.5555554
ACGTAGG450.003816066520.55555527
GTCGCCC555.1248283E-420.1818237
GCACCGA656.869552E-519.92307713
AGGGCAC759.209691E-619.73333210
CCTTAGG902.136063E-618.52
GGTCGCC609.2042814E-418.536
TAAAGCC801.6069467E-518.54
GGCCGTA902.136063E-618.57
Produced by FastQC (version 0.11.5)