##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632990.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	218898
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74507761605862	33.0	33.0	33.0	27.0	33.0
2	30.541028241464062	33.0	33.0	33.0	27.0	33.0
3	30.96535829473088	33.0	33.0	33.0	27.0	33.0
4	31.157785818052243	33.0	33.0	33.0	27.0	33.0
5	31.274282999387843	33.0	33.0	33.0	27.0	33.0
6	33.72804228453435	37.0	33.0	37.0	27.0	37.0
7	33.95570539703424	37.0	33.0	37.0	27.0	37.0
8	34.11490739979351	37.0	33.0	37.0	27.0	37.0
9	34.15710970406308	37.0	33.0	37.0	27.0	37.0
10	34.266009739696116	37.0	37.0	37.0	27.0	37.0
11	34.2507743332511	37.0	37.0	37.0	27.0	37.0
12	34.21706456888597	37.0	37.0	37.0	27.0	37.0
13	34.270249157141684	37.0	37.0	37.0	27.0	37.0
14	34.23828449780263	37.0	37.0	37.0	27.0	37.0
15	34.2811446427103	37.0	37.0	37.0	27.0	37.0
16	34.27070142258038	37.0	37.0	37.0	27.0	37.0
17	34.25635684199947	37.0	37.0	37.0	27.0	37.0
18	34.2567405823717	37.0	37.0	37.0	27.0	37.0
19	34.242967044011365	37.0	37.0	37.0	27.0	37.0
20	34.26281190326088	37.0	37.0	37.0	27.0	37.0
21	34.23153249458652	37.0	37.0	37.0	27.0	37.0
22	34.252030626136374	37.0	37.0	37.0	27.0	37.0
23	34.222144560480224	37.0	37.0	37.0	27.0	37.0
24	34.20111193341191	37.0	37.0	37.0	27.0	37.0
25	34.19788211861232	37.0	37.0	37.0	27.0	37.0
26	34.14944860163181	37.0	37.0	37.0	27.0	37.0
27	34.04611736973385	37.0	33.0	37.0	27.0	37.0
28	34.050425312245885	37.0	33.0	37.0	27.0	37.0
29	34.10044404243072	37.0	33.0	37.0	27.0	37.0
30	34.05388354393371	37.0	33.0	37.0	27.0	37.0
31	34.059598534477246	37.0	33.0	37.0	27.0	37.0
32	34.102842419757145	37.0	33.0	37.0	27.0	37.0
33	34.055770267430496	37.0	33.0	37.0	27.0	37.0
34	33.96737293168508	37.0	33.0	37.0	27.0	37.0
35	33.95728147356303	37.0	33.0	37.0	27.0	37.0
36	33.919473910223026	37.0	33.0	37.0	27.0	37.0
37	33.93699805388811	37.0	33.0	37.0	27.0	37.0
38	33.80429241016364	37.0	33.0	37.0	27.0	37.0
39	33.90038282670468	37.0	33.0	37.0	27.0	37.0
40	33.928181162002396	37.0	33.0	37.0	27.0	37.0
41	33.949734579575875	37.0	33.0	37.0	27.0	37.0
42	33.84184414658882	37.0	33.0	37.0	27.0	37.0
43	33.56308417619165	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	21.0
17	15.0
18	20.0
19	23.0
20	68.0
21	207.0
22	482.0
23	881.0
24	1667.0
25	2718.0
26	3819.0
27	5118.0
28	6785.0
29	8442.0
30	10409.0
31	12356.0
32	14971.0
33	18707.0
34	25208.0
35	40824.0
36	66148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40147465943042	20.745278622920264	12.52272748037899	25.330519237270327
2	16.805544134711145	23.700079489077105	35.56176849491544	23.93260788129631
3	19.24503650101874	25.656698553664263	29.73622417747079	25.36204076784621
4	14.096976674067374	17.93346672879606	37.332456212482526	30.637100384654044
5	15.291596999515756	36.38269879121783	34.55353635026359	13.772167859002824
6	31.241034637136934	39.16892799386015	16.394393735895257	13.195643633107657
7	27.80336046925965	31.84908039360798	22.21948121956345	18.128077917568913
8	25.052307467404912	35.839066597227934	20.078758143062064	19.02986779230509
9	25.590914489853724	15.365604071302617	20.054545952909574	38.98893548593409
10	14.698626757667954	28.778700582006234	33.60880410053998	22.913868559785836
11	34.880629334210454	23.16284296795768	23.685003974453856	18.27152372337801
12	23.85174830286252	25.180677758590758	29.846777951374616	21.12079598717211
13	28.504600316128975	22.023042695684747	25.917550640024125	23.554806348162156
14	22.52190517958136	20.51777540224214	26.68868605469214	30.271633363484362
15	23.758554212464254	29.143253935622987	23.773172893311038	23.325018958601724
16	23.765863552887645	28.41277672706009	24.188891629891547	23.632468090160714
17	22.774534257964895	27.44474595473691	25.799687525696903	23.981032261601293
18	23.589525715173277	26.90933676872333	27.18252336704767	22.318614149055723
19	24.790998547268593	26.0865791373151	26.537017240906724	22.58540507450959
20	24.11762556076346	26.806092335242898	26.81340167566629	22.26288042832735
21	23.49450428966916	26.851318879112647	27.322314502645067	22.331862328573127
22	23.502270463869017	26.423722464344124	26.880099407029757	23.193907664757102
23	23.247357216603167	26.616049484234665	26.990653180933588	23.14594011822858
24	22.865444179480853	26.96141581924001	26.067392118703687	24.105747882575447
25	23.70830249705342	27.196685214117995	26.269312647899934	22.82569964092865
26	23.994737274895154	26.26794214657055	26.66493069831611	23.072389880218182
27	23.507752469186563	26.59686246562326	26.521941726283476	23.373443338906704
28	23.05822803314786	26.046834598762896	27.5123573536533	23.38258001443595
29	23.75535637602902	26.256978135935462	26.31865069575784	23.669014792277682
30	23.402223866823817	26.84081170225402	26.95867481658124	22.798289614340923
31	23.648457272336888	26.40133760929748	27.02217471150947	22.928030406856163
32	23.376641175341938	26.340578717028023	26.807919670348745	23.474860437281293
33	23.485824447916382	26.317280194428456	27.21267439629416	22.984220961361
34	23.889208672532412	26.008917395316537	26.667671700974882	23.434202231176165
35	23.24370254639147	27.195314712788605	26.91755977669965	22.643422964120276
36	24.1738161152683	25.675885572275668	27.101206954837416	23.049091357618618
37	23.728403183217754	26.1624135442078	26.600517135834956	23.508666136739485
38	23.835302286909883	26.362963572074666	26.78507798152564	23.016656159489806
39	23.763579384005336	26.325046368628314	26.70878674085647	23.202587506509882
40	23.178375316357393	27.252418934846368	26.887865581229615	22.68134016756663
41	23.907025189814433	25.990187210481597	26.765890962914234	23.33689663678974
42	23.451561914681722	26.644373178375318	26.706959405750624	23.197105501192336
43	23.641147931913494	25.876435600142532	26.897002256758856	23.585414211185117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	5.5
2	10.0
3	39.0
4	68.0
5	68.0
6	75.5
7	83.0
8	84.0
9	85.0
10	110.0
11	135.0
12	135.0
13	217.0
14	299.0
15	574.5
16	850.0
17	955.0
18	1060.0
19	1060.0
20	1099.5
21	1139.0
22	1270.0
23	1401.0
24	1805.0
25	2209.0
26	2209.0
27	2957.5
28	3706.0
29	4592.0
30	5478.0
31	6601.5
32	7725.0
33	7725.0
34	9153.0
35	10581.0
36	11825.5
37	13070.0
38	13962.5
39	14855.0
40	14855.0
41	15413.5
42	15972.0
43	16896.0
44	17820.0
45	17842.0
46	17864.0
47	17864.0
48	17270.5
49	16677.0
50	16857.0
51	17037.0
52	16544.5
53	16052.0
54	16052.0
55	14901.0
56	13750.0
57	12142.5
58	10535.0
59	10080.5
60	9626.0
61	9626.0
62	8891.5
63	8157.0
64	6591.5
65	5026.0
66	4110.0
67	3194.0
68	3194.0
69	2471.0
70	1748.0
71	1470.0
72	1192.0
73	936.5
74	681.0
75	681.0
76	520.5
77	360.0
78	264.0
79	168.0
80	126.0
81	84.0
82	84.0
83	80.5
84	77.0
85	67.0
86	57.0
87	49.0
88	41.0
89	41.0
90	30.5
91	20.0
92	12.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	218898.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.88518058635103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30564884283498	45.996660488586265
2	8.687651194097429	9.710219117067258
3	2.9723608386069995	4.983327667000497
4	1.465519798213897	3.276033543042254
5	0.915810399963214	2.5590114792401293
6	0.6203997719418984	2.0802691974422394
7	0.4506065610247902	1.76275603253855
8	0.3574325825009351	1.5980147536408456
9	0.2821630741621628	1.4191860918917183
>10	1.7929151042914582	18.90968264326211
>50	0.11583141977737316	4.4289269054456915
>100	0.03267040045002832	2.962207951499842
>500	9.900121348493432E-4	0.31370412934262426
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	567	0.2590247512540087	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	431	0.19689535765516358	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	274	0.12517245475061445	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	266	0.12151778453891766	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	263	0.12014728320953137	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	220	0.10050343082166123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	4.568337764620965E-4	0.0	0.0
4	0.0	0.0	4.568337764620965E-4	0.0	0.0
5	0.0	0.0	4.568337764620965E-4	0.0	0.0
6	0.0	0.0	9.13667552924193E-4	0.0	0.0
7	0.0	0.0	0.0013705013293862896	0.0	0.0
8	0.0	0.0	0.0013705013293862896	0.0	0.0
9	0.0	0.0	0.0022841688823104825	0.0	0.0
10	0.0	0.0	0.0027410026587725792	0.0	0.0
11	0.0	0.0	0.003654670211696772	0.0	0.0
12	0.0	0.0	0.003654670211696772	0.0	0.0
13	0.0	0.0	0.003654670211696772	0.0	0.0
14	0.0	0.0	0.003654670211696772	0.0	0.0
15	0.0	0.0	0.003654670211696772	0.0	0.0
16	0.0	0.0	0.003654670211696772	0.0	0.0
17	0.0	0.0	0.003654670211696772	0.0	0.0
18	0.0	0.0	0.004111503988158869	0.0	0.0
19	0.0	0.0	0.004111503988158869	0.0	0.0
20	0.0	0.0	0.004111503988158869	0.0	4.568337764620965E-4
21	0.0	0.0	0.005025171541083061	0.0	4.568337764620965E-4
22	0.0	0.0	0.0054820053175451584	4.568337764620965E-4	4.568337764620965E-4
23	0.0	0.0	0.0054820053175451584	4.568337764620965E-4	4.568337764620965E-4
24	0.0	0.0	0.005938839094007255	4.568337764620965E-4	4.568337764620965E-4
25	0.0	0.0	0.005938839094007255	4.568337764620965E-4	4.568337764620965E-4
26	0.0	0.0	0.005938839094007255	4.568337764620965E-4	4.568337764620965E-4
27	0.0	0.0	0.005938839094007255	9.13667552924193E-4	4.568337764620965E-4
28	0.0	0.0	0.005938839094007255	0.007309340423393544	4.568337764620965E-4
29	0.0	0.0	0.005938839094007255	0.017816517282021764	4.568337764620965E-4
30	0.0	0.0	0.005938839094007255	0.03015102924649837	4.568337764620965E-4
31	0.0	0.0	0.005938839094007255	0.07903224332794269	4.568337764620965E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTA	20	0.001838682	37.0	5
GTGTCGA	25	0.0054867463	29.599998	13
GGTATCA	90	5.456968E-12	26.722221	1
TGTCGAA	35	8.846375E-4	26.42857	14
CCCACAC	60	1.3291574E-6	24.666668	3
GCTCTAG	55	1.8935485E-5	23.545456	1
GTATCAA	310	0.0	22.67742	1
GACGTAT	50	2.6924285E-4	22.199999	26
ACGGGAG	50	2.6924285E-4	22.199999	5
ACGTATG	50	2.6924285E-4	22.199999	27
ACCCCAC	70	5.0708477E-6	21.142857	1
TAATAGT	45	0.0038160665	20.555555	4
ACGTAGG	45	0.0038160665	20.555555	27
GTCGCCC	55	5.1248283E-4	20.18182	37
GCACCGA	65	6.869552E-5	19.923077	13
AGGGCAC	75	9.209691E-6	19.733332	10
CCTTAGG	90	2.136063E-6	18.5	2
GGTCGCC	60	9.2042814E-4	18.5	36
TAAAGCC	80	1.6069467E-5	18.5	4
GGCCGTA	90	2.136063E-6	18.5	7
>>END_MODULE