FastQCFastQC Report
Fri 10 Feb 2017
ERR1632989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504447
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46060.9130790747095333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.6676618158101842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17890.3546457804288657No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11060.21924999058374814No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9990.19803864429761697No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9660.19149682721871672No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9480.1879285633574984No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9410.18654090518924685No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9240.1831708782092073No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9190.18217969380331334No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9120.18079203563506177No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9050.1794043774668102No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9010.17861142994209503No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8810.1746466923185191No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.17206961286319475No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8290.16433837449722172No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8170.16195953192307616No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8150.16156305816071856No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7780.15422829355710313No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7360.1459023445475937No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7290.14451468637934214No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7270.14411821261698454No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7260.14391997573580576No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7150.14173937004283899No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7080.14035171187458742No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6820.13519755296393873No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6740.13361165791450838No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6660.132025762865078No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6610.131034578459184No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6560.13004339405329005No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6480.1284574990038597No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6480.1284574990038597No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6470.12825926212268088No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6410.1270698408356081No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6110.12112273440024422No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA5870.11636504925195312No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5820.11537386484605915No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5810.11517562796488036No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC5740.1137879697966288No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5690.11279678539073479No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5580.11061617969776805No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5530.10962499529187407No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5440.1078408633612649No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5420.10744438959890731No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5400.10704791583654973No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5360.10625496831183454No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5190.102884941331795No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5120.10149728316354344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.030.689921
TTTAGCG250.005493455629.626
TAGCGGG250.005493455629.628
ATAGATC250.005493455629.66
CGTTATT250.005493455629.610
GTATCAA21350.025.3021071
GTATAGA701.91847E-723.7857151
AGACTAT400.001929692723.1250026
ATTAGAC400.001929692723.1250023
TTAGACT400.001929692723.1250024
CCACATT652.6778944E-622.76922815
TAAACTG502.69919E-422.25
CCGTTAA1950.021.82051316
CGTTAAA2050.020.756117
AACATGT2050.020.756122
AGTACAG450.003822876820.5555555
CACTGTG555.1375956E-420.1818187
CCGGCGG656.8923226E-519.92307523
GTGCTAG656.8923226E-519.92307527
GGCCGTT2150.019.79069714
Produced by FastQC (version 0.11.5)