FastQCFastQC Report
Fri 10 Feb 2017
ERR1632988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414476
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9630.23234155898049585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.14427855895154362No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4790.11556760825717292No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4270.10302164660921259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG303.595542E-430.83333421
GCCTAAG250.00549233829.61
GGTATCA2550.026.843141
CGCCGTC358.859791E-426.4285726
GTCCGGC502.6980636E-422.215
GTATCAA6850.021.6058391
GTCTAGA603.7188893E-521.5833341
TAACCGT1001.2838427E-820.357
ATAAGGC656.888531E-519.9230753
CTAACCG1052.2489985E-819.3809536
TGGCACG500.00702747818.519
GCCTAAA701.21681216E-418.511
CGCGTGT500.00702747818.514
CTGTGCG500.00702747818.59
TGCCGCG500.00702747818.511
TGTCCGG500.00702747818.514
GTACCCT1156.375558E-817.6956521
GTATATA953.5951398E-617.5263161
TAGACAG752.063251E-417.2666665
ATAGCAG752.063251E-417.2666665
Produced by FastQC (version 0.11.5)